Lipid biosynthesis.

Ohlrogge, John B.; Browse, John

doi:10.1105/tpc.7.7.957

Cited by 1,286 publications

(1,102 citation statements)

References 69 publications

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“…Extensive exchange of acyl-CoA derivatives occurs between the chloroplasts and the endoplasmic reticulum (ER) via the cytosol (Ohlrogge and Browse, 1995). De novo fatty acid biosynthesis in higher plants occurs in the chloroplasts (Ohlrogge and Browse, 1995), and the majority of plastid-synthesized fatty acids are exported as palmitoyl-CoA and oleoyl-CoA to the ER for glycerolipid biosynthesis (Browse et al, 1986;Maréchal et al, 1997).…”

mentioning

confidence: 99%

“…De novo fatty acid biosynthesis in higher plants occurs in the chloroplasts (Ohlrogge and Browse, 1995), and the majority of plastid-synthesized fatty acids are exported as palmitoyl-CoA and oleoyl-CoA to the ER for glycerolipid biosynthesis (Browse et al, 1986;Maréchal et al, 1997). However, it is not clear how these acyl-CoAs are transported from the chloroplasts to the ER and whether shuttle proteins are involved in this process.…”

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confidence: 99%

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Overexpression of the Arabidopsis 10-Kilodalton Acyl-Coenzyme A-Binding Protein ACBP6 Enhances Freezing Tolerance

2008

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Small 10-kD acyl-coenzyme A-binding proteins (ACBPs) are highly conserved proteins that are prevalent in eukaryotes. In Arabidopsis (Arabidopsis thaliana), other than the 10-kD ACBP homolog (designated Arabidopsis ACBP6), there are five larger forms of ACBPs ranging from 37.5 to 73.1 kD. In this study, the cytosolic subcellular localization of Arabidopsis ACBP6 was confirmed by analyses of transgenic Arabidopsis expressing autofluorescence-tagged ACBP6 and western-blot analysis of subcellular fractions using ACBP6-specific antibodies. The expression of Arabidopsis ACBP6 was noticeably induced at 48 h after 4°C treatment by northern-blot analysis and western-blot analysis. Furthermore, an acbp6 T-DNA insertional mutant that lacked ACBP6 mRNA and protein displayed increased sensitivity to freezing temperature (28°C), while ACBP6-overexpressing transgenic Arabidopsis plants were conferred enhanced freezing tolerance. Northern-blot analysis indicated that ACBP6-associated freezing tolerance was not dependent on the induction of cold-regulated COLD-RESPONSIVE gene expression. Instead, ACBP6 overexpressors showed increased expression of mRNA encoding phospholipase Dd. Lipid profiling analyses of rosettes from cold-acclimated, freezingtreated (28°C) transgenic Arabidopsis plants overexpressing ACBP6 showed a decline in phosphatidylcholine (236% and 246%) and an elevation of phosphatidic acid (73% and 67%) in comparison with wild-type plants. From our comparison, the gain in freezing tolerance in ACBP6 overexpressors that was accompanied by decreases in phosphatidylcholine and an accumulation of phosphatidic acid is consistent with previous findings on phospholipase Dd-overexpressing transgenic Arabidopsis. In vitro filterbinding assays indicating that histidine-tagged ACBP6 binds phosphatidylcholine, but not phosphatidic acid or lysophosphatidylcholine, further imply a role for ACBP6 in phospholipid metabolism in Arabidopsis, including the possibility of ACBP6 in the cytosolic trafficking of phosphatidylcholine.

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Overexpression of the Arabidopsis 10-Kilodalton Acyl-Coenzyme A-Binding Protein ACBP6 Enhances Freezing Tolerance

2008

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“…Balance between the 2 pathways is dependent on the ratio of the activities of the PA phosphatases and the CDP-DAG synthases. CDP-DAG and DAG are substrates in formation of phospholipids and galactolipids [14]. DAG can also generate TAG ( [83,84]).…”

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

“…The ER glycerol-3-P and lyso-PA acyltransferases are involved in formation of most phospholipids: PC (phosphatidylcholine), PE (phosphatidylethanolamine), PI (phosphatidylinositol), PS (phosphatidylserine), and part of the cellular PG (phosphatidylgycerol) [14]. Furthermore, since about half of the galactolipids derive from ER-synthesized PC (referred to as eukaryotic galactolipids) these acyltransferases also display a role in formation of galactolipids.…”

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

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Role of phosphatidic acid in plant galactolipid synthesis

Dubots¹,

Botté²,

Boudière³

et al. 2012

Biochimie

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Phosphatidic acid (PA) is a precursor metabolite for phosphoglycerolipids and also for galactoglycerolipids, which are essential lipids for formation of plant membranes. PA has in addition a main regulatory role in a number of developmental processes notably in the response of the plant to environmental stresses. We review here the different pools of PA dispatched at different locations in the plant cell and how these pools are modified in different growth conditions, particularly during plastid membrane biogenesis and when the plant is exposed to phosphate deprivation. We analyze how these modifications can affect galactolipid synthesis by tuning the activity of MGD1 enzyme allowing a coupling of phosphoand galactolipid metabolisms. Some mechanisms are considered to explain how physicochemical properties of PA allow this lipid to act as a central internal sensor in plant physiology.

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Natural Deuterium Distribution in Long-Chain Fatty Acids Is Nonstatistical: A Site-Specific Study by Quantitative2H NMR Spectroscopy

et al. 2001

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Quantitative (2)H NMR spectroscopy has been used to study the site-specific natural occurrence of (2)H in common unsaturated fatty acids. A marked nonstatistical isotopic distribution of (2)H is observed in both methyl oleate and methyl linoleate. By chemical modification, the internal isotopic (2)H signatures of these products have been partially accessed. Notably, it can be seen that (1) the sites of desaturation show a strong impoverishment at only one ethylenic position of each desaturation; (2) the level of impoverishment reflects the source of the hydrogen atoms present; and (3) a gradient of (2)H content occurs along the chain. These effects can in part be related to the mechanisms proposed for the enzymes responsible for the various steps of biosynthesis of unsaturated fatty acids in plants.

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Lipid biosynthesis.

Cited by 1,286 publications

References 69 publications

Overexpression of the Arabidopsis 10-Kilodalton Acyl-Coenzyme A-Binding Protein ACBP6 Enhances Freezing Tolerance

Overexpression of the Arabidopsis 10-Kilodalton Acyl-Coenzyme A-Binding Protein ACBP6 Enhances Freezing Tolerance

Role of phosphatidic acid in plant galactolipid synthesis

Natural Deuterium Distribution in Long-Chain Fatty Acids Is Nonstatistical: A Site-Specific Study by Quantitative2H NMR Spectroscopy

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