Lipid Cell Biology: A Focus on Lipids in Cell Division

Storck, Elisabeth M.; Özbalci, Cagakan; Eggert, Ulrike

doi:10.1146/annurev-biochem-062917-012448

Cited by 76 publications

(62 citation statements)

References 159 publications

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“…3f, g , these results further suggest that the dissociated lipid in the cristae would slowly pass the outer membrane and be released into the cytoplasm. Dramatic membrane rearrangements occur during cell division 4 , 21 , 22 , and the high spatiotemporal monitoring of lipid properties by SPOT can provide insights on how the lipid is regulated in cytokinesis.…”

Section: Resultsmentioning

confidence: 99%

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Zhanghao

Liu

et al. 2020

Nat Commun

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Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles’ biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one dye that universally stains the lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, and even within the same organelle. Furthermore, we obtain real-time monitoring of the multi-organelle interactive activities of cell division and successfully reveal their sophisticated lipid dynamics during the plasma membrane separation, tunneling nanotubules formation, and mitochondrial cristae dissociation. This work suggests research frontiers in correlating single-cell super-resolution lipidomics with multiplexed imaging of organelle interactome.

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Section: Resultsmentioning

confidence: 99%

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Zhanghao

Liu

et al. 2020

Nat Commun

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“…Lipids can serve as signaling molecules and key regulators in fundamental cellular processes such as cell death, [ 10,15 ] proliferation, [ 16 ] and senescence. [ 13,14 ] Previous studies have shown that changes in lipid metabolism occur during TIS.…”

Section: Resultsmentioning

confidence: 99%

Untargeted Lipidomics Highlight the Depletion of Deoxyceramides during Therapy‐Induced Senescence

2020

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Therapy-induced senescence is a state of cell cycle arrest that occurs as a response to various chemotherapeutic reagents, especially ones that cause DNA damage. Senescent cells display resistance to cell death and can impair the efficacy of chemotherapeutic strategies. Since lipids can exhibit pro-survival activity, it is envisioned in this article that probing the lipidome could provide insights into novel lipids that are involved in senescence. Therefore, a tissue culture model system is established and the cellular lipidomes of senescent and proliferating cells are comparatively analyzed. Out of thousands of features detected, 17 species are identified that show significant changes in senescent cells. The majority of these species (11 out of 17) are atypical sphingolipids, 1-deoxyceramides/dihydroceramides, which are produced as a result of the utilization of alanine, instead of serine during sphingolipid biosynthesis. These lipids are depleted in senescent cells. Elevating the levels of deoxyceramides by supplementing the growth medium with metabolic precursors or by directly adding deoxyceramide result in decreased senescence, suggesting that these species might play a key role in this process.

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“…Whether through their structural or signaling roles, regulation of lipids has been linked to a plethora of cellular programs and diseases. Recent reviews highlight that regulation of specific lipids is required for fundamental processes such as cellular division [ 22 ] and cell death [ 16 , 23 ]. These key roles require tight control of lipid biosynthesis and metabolism in order to maintain cellular homeostasis [ 16 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Lipid Players of Cellular Senescence

Millner

Atilla‐Gokcumen

2020

Metabolites

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Lipids are emerging as key players of senescence. Here, we review the exciting new findings on the diverse roles of lipids in cellular senescence, most of which are enabled by the advancements in omics approaches. Senescence is a cellular process in which the cell undergoes growth arrest while retaining metabolic activity. At the organismal level, senescence contributes to organismal aging and has been linked to numerous diseases. Current research has documented that senescent cells exhibit global alterations in lipid composition, leading to extensive morphological changes through membrane remodeling. Moreover, senescent cells adopt a secretory phenotype, releasing various components to their environment that can affect the surrounding tissue and induce an inflammatory response. All of these changes are membrane and, thus, lipid related. Our work, and that of others, has revealed that fatty acids, sphingolipids, and glycerolipids are involved in the initiation and maintenance of senescence and its associated inflammatory components. These studies opened up an exciting frontier to investigate the deeper mechanistic understanding of the regulation and function of these lipids in senescence. In this review, we will provide a comprehensive snapshot of the current state of the field and share our enthusiasm for the prospect of potential lipid-related protein targets for small-molecule therapy in pathologies involving senescence and its related inflammatory phenotypes.

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Lipid Cell Biology: A Focus on Lipids in Cell Division

Cited by 76 publications

References 159 publications

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Untargeted Lipidomics Highlight the Depletion of Deoxyceramides during Therapy‐Induced Senescence

Lipid Players of Cellular Senescence

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