Lipid Changes After Incubation of Normal Plasma

Berlin, Ragnar; Oldfelt, Carl Olof; Vikrot, Olle

doi:10.1111/j.0954-6820.1969.tb07359.x

Cited by 16 publications

(2 citation statements)

References 18 publications

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“…Plasma was separated by centrifugation at +4"C. Concentrations of individual phospholipids, i.e. phosphatidylcholine (PC), lysolecithin (LL), sphingomyelin (SP) and phosphatidylethanolamine (PE), and concentrations of triglycerides (TG), free fatty acids (FFA), total cholesterol (TC) and unesteritied cholesterol (UC) in plasma, were determined before and after 6 hours' incubation at 37°C and before and after Blz treatment as previously described (5,10). In 17 cases the same determinations were made before treatment and after i.v.…”

Section: Methodsmentioning

confidence: 99%

Studies on Plasma Lipid and Phospholipid Composition in Pernicious Anemia before and after Specific Treatment

Wallentin

Berlin

Vikrot

1977

Journal of Internal Medicine

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Patients with pernicious anemia (PA) have been compared with a reference group concerning the concentration of lipids in plasma before (34 cases) and after (15 cases) treatment with vitamin B12. The lipid parameters in plasma and in postheparin plasma have been measured before and after 6 hours' incubation at 37°C before and after specific treatment. In a limited number of cases the lecithin: cholesterol acyl transfer (LCAT) rate has been determined. In relapse, the PA cases showed increased free fatty acid (FFA) and triglyceride (TG) concentrations but decreased concentrations of total cholesterol (TC), unesterified cholesterol (UC) and all examined phospholipid fractions. After treatment, FFA remained unchanged and TG decreased, while TC and possibly also UC and the phospholipid fractions were in line with reference levels. After incubations, UC and phosphatidylcholine (PC) decreased and lysolecithin (LL) increased. FFA increased and TG decreased. Incubation of postheparin plasma resulted in an augmented decrease in TG and PC and increase in FFA and LL. In relapse, the changes on incubation were less pronounced than after treatment. The LCAT rate was low but within the normal range before treatment in the six cases examined. After treatment, LCAT rates increased but were still normal in relation to the plasma lipid concentrations. The study showed a decreased net esterification of cholesterol and LL formation on incubation of plasma in PA cases in relapse. This finding might be explained by the low concentration of plasma substrates for the LCAT reaction and phospholipases. After treatment the concentration of lipid substrates was restored to normal, with subsequent normal LL formation and cholesterol esterification. The observations could also explain the frequent lack of stabilization of erythrocytes suspended in plasma of PA after incubation, resulting in virtually the same ESR after as before this procedure. Due to low LL formation following the insufficient substrate availability for LCAT and phospholipase, the previously reported critical level of the LL concentration is not reached and cannot achieve its normal reduction of the ESR.

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Section: Methodsmentioning

confidence: 99%

Studies on Plasma Lipid and Phospholipid Composition in Pernicious Anemia before and after Specific Treatment

Wallentin

Berlin

Vikrot

1977

Journal of Internal Medicine

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“…In a publication in 1969 Berlin et al ( 5 ) showed that the lysolecithin concentration in normal plasma is remarkably constant in the same individual as well as in different age groups (0.19-0.21 mM lysolecithin phosphorus/l corresponding to 5.9-6.4 pg/ml) with the exception of young men, who have higher values (0.27 mM lysolecithin phosphorus/l= 8.4 ,%g/ml). After the incubation of plasma at 37°C during 6 hours the Acia med.…”

Section: Introductionmentioning

confidence: 99%

Lysolecithin as a Factor Influencing Erythrocyte Sedimentation Rate

Berlin

Böttiger

Vikrot

1973

Journal of Internal Medicine

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In an experimental study increasing amounts of lysolecithin have been added to aliquots of plasma from 18 patients with a high erythrocyte sedimentation rate (ESR) and the final concentration of lysolecithin in the samples has been determined by means of thin layer chromatography and subsequent phosphorus determination as well as determination of the ESR. It was found that the ESR remained high in the different plasma samples until a critical level of lysolecithin was reached, whereafter the ESR abruptly dropped to lower values. This change took place within a very narrow concentration range, usually between 4.5 and 5.5 ,ug lysolecithin P/ml, and at a concentration of 7-8 pg/ml the ESR was consistently very low. In additional experiments, using decreasing amounts of red cells to the various plasma samples, the ESR had a normal tendency to increase inversely to the hematocrit, but in this case no threshold level could be observed. These findings clearly demonstrate that the lysolecithin concentration is of considerable importance for the ESR value. A low lysolecithin content of the plasma means a higher ESR value than expected from the amount of protein components, and a high lysolecithin concentration means a low ESR in spite of an unchanged protein pattern. Normally a decrease of the ESR takes place on incubation of a plasma sample due to the enzymatic formation of lysolecithin from lecithin. This phenomenon is explained by an increase of the lysolecithin amount above the demonstrated critical level. In a number of pathological conditions this "heat-stabilization" process does not occur, and in these cases the lysolecithin concentration is characteristically low and does not rise above the critical level even after incubation. A number of possible explanations of this drastical decrease of the ESR at a certain lysolecithin level are discussed in this presentation, but the most likely is that lysolecithin is bound to plasma proteins, e.g. albumin. In this form it is unable to exert its physiological effect on the red cell surface membranes until the saturation limit of the carrier protein is reached and "free" lysolecithin appears.Since Fkhraeus (9) described the sedimentation of red cells in plasma in 1921, an enormous num-ber of papers have dealt with factors affecting the erythrocyte sedimentation rate (ESR) and especially the plasma constituents enhancing this phenomenon. Fihraeus stressed in the first place the importance of fibrinogen and globulin fractions in this context. He maintained that in acute febrile or inflammatory states the increased ESR could be referred to a raised plasma fibrinogen concentration ("acute ESR increase"), whereas a globulin increase was generally found in morbid conditions of a chronic type ("chronic ESR increase").In his work Fihrsus was also able to show that incubation at body temperature of a plasma with a high ESR caused a considerable reduction of the ESR compared with the basal value of a non-incubated plasma sample. In collaboration with Bergenhem (1) he later discussed ...

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Comparative In Vivo and In Vitro Studies of Phenytoin Protein Binding and In Vitro Lipolysis in Plasma of Pregnant and Nonpregnant Rats

Chou

Levy

1984

Journal of Pharmaceutical Sciences

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Lipid Changes After Incubation of Normal Plasma

Cited by 16 publications

References 18 publications

Studies on Plasma Lipid and Phospholipid Composition in Pernicious Anemia before and after Specific Treatment

Studies on Plasma Lipid and Phospholipid Composition in Pernicious Anemia before and after Specific Treatment

Lysolecithin as a Factor Influencing Erythrocyte Sedimentation Rate

Comparative In Vivo and In Vitro Studies of Phenytoin Protein Binding and In Vitro Lipolysis in Plasma of Pregnant and Nonpregnant Rats

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