Lipid changes in central nervous system membranes in experimental allergic encephalomyelitis (EAE)

Abstract

Lipid composition of myelin fractions isolated from Lewis rats during the early stage of the development of experimental allergic encephalomyelitis (EAE) were determined by high-performance thin layer chromatography (HPTLC). When comparing the myelin fractions of EAE-affected animals with those of controls, the main differences were observed in the light fraction, where a decrease in the percentage of phospholipids (PH) relative to the total lipids was observed. These findings give further support that the light myelin fraction being the most sensitive at the onset of clinical symptoms must play a key role in demyelinating process.

Increasing acetyl‐CoA metabolism attenuates injury and alters spinal cord lipid content in mice subjected to experimental autoimmune encephalomyelitis

“…Specifically, in white matter, there is a decrease in PtdSer, PtdIns, EtnGpl, and CerPCho in EAE mice (Salvati et al . ). The question remains whether this loss in lipid content is a result of a metabolic dysfunction or the result of immune‐targeted degradation of myelin membranes.…”

“…However, there is no information to suggest that vehicle will result in significant or sustained changes in lipid metabolism or content within the spinal cord (Salvati et al . ; Revina et al . ).…”

“…It is well known that phospholipid, cholesterol, plasmalogen, and cerebroside levels are decreased in patients who suffer from multiple sclerosis (Cumings 1953(Cumings , 1969Gerstl et al 1961Gerstl et al , 1970. Specifically, in white matter, there is a decrease in PtdSer, PtdIns, EtnGpl, and CerPCho in EAE mice (Salvati et al 1990). The question remains whether this loss in lipid content is a result of a metabolic dysfunction or the result of immune-targeted degradation of myelin membranes.…”

“…Furthermore, in multiple sclerosis there is a significant decrease in overall lipid content within the white matter tracks and a significant increase in enzyme activity of those enzymes that metabolize membrane lipid (Cumings ; Salvati et al . ). It is also suggested that changes in energy metabolism in multiple sclerosis lesions may contribute to changes in myelin pathology (Rone et al .…”

“…However, another factor involved in multiple sclerosis, as well as other demyelinating diseases, is the disruption of oligodendrocyte maturation and dysfunction in mature oligodendrocyte function (Lucchinetti et al 2000;Wolswijk 2000;de Castro et al 2013). Furthermore, in multiple sclerosis there is a significant decrease in overall lipid content within the white matter tracks and a significant increase in enzyme activity of those enzymes that metabolize membrane lipid (Cumings 1969;Salvati et al 1990). It is also suggested that changes in energy metabolism in multiple sclerosis lesions may contribute to changes in myelin pathology (Rone et al 2016).…”