Lipid changes in maturing oil‐bearing plants. II. Changes in fatty acid composition of flax and safflower seed oils

Abstract

Changes in the fatty acids composition of the oil in flax and safflower seed that occur during the seed‐ripening period have been measured. Concentrations of lipid or of specific fatty acid, expressed on a weight‐per‐seed basis, have been plotted as functions of days after fertilization and of percentage of oil development. Relations between these two independent variables have been established, and limitations to the unsefulness of the latter variable have been pointed out. Days after fertilization proved to be the more useful abscissa. Nonpolar solvents were used to remove free lipid from the tissue, and the total extractable matter was separated into true lipid and nonlipid components. With both flax and safflower, weight of true free lipid per seed and total unsaturation increased during the same period of growth. Nonlipid extractable matter was an inverse function of the extent of development. In developing flax seed, oleic, linoleic, and linolenic acids all increased continuously; oil in immature seed however was more saturated than oil in more mature seed. Nevertheless the ratio of linolenic acid to linoleic acid that characterizes linseed oil was established by the 20th day after fertilization during a normal growing season. In developing safflower seed, oleic acid concentration increased slowly during the first 30 days after fertilization and then appeared to level off in some cases as maturity was approached. Initially linoleic acid was present in almost the same amount as oleic acid, but by the 20th day after fertilization its concentration was three times that of oleic acid. This ratio of linoleic to oleic acid tended to increase steadily during the latter part of seed development.

Fat Metabolism in Higher Plants XXVI. Biosynthesis of Fatty Acids in Tissues of Developing Seeds and Germinating Seedlings of Safflower (Carthamus tinctorius L.)

“…fnitial studies on the rate of acetate-1-C14 incorporation into developing seed tissue confirml the data of Sims and coworkers (18) as indicated in figure 7. Initially there is a decrease in rate of C14 label enterinig total fatty acids, with a rapid increase from 10 to 20 days after flowering which is followed by a decrease in rate at 20 days after flowering.…”

Fatty Acid Accumulation in Maturing Flaxseeds as Influenced by Environment

“…Stuidies by this technique provided early insights into fatty acid biosynthesis in oilseeds (14,23), and the method ' still is commonly employed for this puirpose since fatty acid composition can readily be determined by gas chromatography ( 1,12,13,20). Responses to environment other than changes in fatty acids were also measuired in the present stuidies.…”

Fatty Acid Composition of the Oil from Developing Seeds of Different Varieties of Safflower (Carthamus tinctorius L.)

“…A slower loss of moisture from the seeds of the Montola-2001 variety was observed compared with the Centennial variety. Similarly, numerous research studies have shown that the moisture content decreases during safflower seed development [27][28][29].…”