Lipid composition and fluidity in the jejunal brush-border membrane of spontaneously hypertensive rats. Effects on activities of membrane-bound proteins

Vázquez, Carmen M.; Zanetti, Rosana; Ruı́z-Gutı́errez, Valentina

doi:10.1007/bf01207336

Cited by 17 publications

(10 citation statements)

References 43 publications

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“…Previous studies of cell membranes from SHR rats and humans with hypertension reported altered membrane fluidity [4,6,33]. 2OHOA treatment was accompanied by an increase in the fluidity of liposomes reconstituted from liver plasma membranes from SHR rats that was in agreement with data previously reported [6] but not explainable with the lipid composition found.…”

Section: Discussionsupporting

confidence: 82%

“…2OHOA treatment was accompanied by an increase in the fluidity of liposomes reconstituted from liver plasma membranes from SHR rats that was in agreement with data previously reported [6] but not explainable with the lipid composition found. The increase in fluidity should diminish restrictions to the lateral diffusion of components within the membrane, and possibly favour the formation of microdomains.…”

Section: Discussionsupporting

confidence: 82%

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Effects of 2-hydroxyoleic acid on the structural properties of biological and model plasma membranes

Prades

Alemany

Perona

et al. 2008

Molecular Membrane Biology

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This article maybe used for research, teaching and private study purposes. Any substantial or systematic reproduction, re-distribution, re-selling, loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden.The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material. AbstractGenetic hypertension is associated with alterations in lipid metabolism, membrane lipid composition and membraneprotein function. 2-Hydroxyoleic acid (2OHOA) is a new antihypertensive molecule that regulates the structure of model membranes and their interaction with certain peripheral signalling proteins in vitro. While the effect of 2OHOA on elevated blood pressure is thought to arise through its influence on signalling proteins, its effects on membrane lipid composition remain to be assessed. 2OHOA administration altered the lipid membrane composition of hypertensive and normotensive rat plasma membranes, and increased the fluidity of reconstituted liver membranes from hypertensive rats. In spontaneously hypertensive rats (SHR), treatment with 2OHOA increased the cholesterol and sphingomyelin content while decreasing that of phosphatidylserine-phosphatidylinositol lipids. In addition, monounsaturated fatty acid levels increased as well as the propensity of reconstituted membranes to form H II -phases. These data suggest that 2OHOA regulates lipid metabolism that is altered in hypertensive animals, and that it affects the structural properties of liver plasma membranes in SHR. These changes in the structural properties of the plasma membrane may modulate the activity of signalling proteins that associate with the cell membrane such as the Gaq/11 protein and hence, signal transduction.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 82%

Effects of 2-hydroxyoleic acid on the structural properties of biological and model plasma membranes

Prades

Alemany

Perona

et al. 2008

Molecular Membrane Biology

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“…21 In the present study, the changes in the lipid composition that result in a reduction of the cholesterol:phospholipid ratio could be associated with an increase in the membrane fluidity. Although fatty acid composition also contributes to the modulation of membrane fluidity, 22 only minor changes were found in the phospholipid fatty acid profile of the 3 groups of patients analyzed.…”

Section: Barceló Et Al Mediterranean Diet In Hypertensive Patientsmentioning

confidence: 99%

“…Low-membrane PE concentrations have been reported in spontaneously hypertensive rats compared with normotensive animals. 22,23 In this latter strain, VOO consumption led to increased PE content in membranes compared with high-oleic sunflower oil. 9 It is worth noting that, in contrast with VOO, high-oleic sunflower oil was unable to exhibit beneficial effects on blood pressure in hypertensive subjects.…”

Section: Barceló Et Al Mediterranean Diet In Hypertensive Patientsmentioning

confidence: 99%

Mediterranean-Style Diet Effect on the Structural Properties of the Erythrocyte Cell Membrane of Hypertensive Patients

et al. 2009

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Abstract-A currently ongoing randomized trial has revealed that the Mediterranean diet, rich in virgin olive oil or nuts, reduces systolic blood pressure in high-risk cardiovascular patients. Here, we present a structural substudy to assess the effect of a Mediterranean-style diet supplemented with nuts or virgin olive oil on erythrocyte membrane properties in 36 hypertensive participants after 1 year of intervention. Erythrocyte membrane lipid composition, structural properties of reconstituted erythrocyte membranes, and serum concentrations of inflammatory markers are reported. After the intervention, the membrane cholesterol content decreased, whereas that of phospholipids increased in all of the dietary groups; the diminishing cholesterol:phospholipid ratio could be associated with an increase in the membrane fluidity. Moreover, reconstituted membranes from the nuts and virgin olive oil groups showed a higher propensity to form a nonlamellar inverted hexagonal phase structure that was related to an increase in phosphatidylethanolamine lipid class. These data suggest that the Mediterranean-style diet affects the lipid metabolism that is altered in hypertensive patients, influencing the structural membrane properties. The erythrocyte membrane modulation described provides insight in the structural bases underlying the beneficial effect of a Mediterranean-style diet in hypertensive subjects. Key Words: Mediterranean diet Ⅲ lipids Ⅲ membrane structure Ⅲ cardiovascular disease Ⅲ hypertension C ardiovascular disease constitutes the main cause of death in industrialized countries, 1 and hypertension is one of the main modifiable cardiovascular risk factors, especially in the elderly. 2 Healthy diet and lifestyle constitute the first steps in the guidelines for management of hypertension. 3 In this context, the type and amount of dietary lipids influence the lipid composition of cell membranes 4,5 and modulate the interactions with proteins involved in the regulation of blood pressure. 6 Thus, the changes in membrane properties induced by dietary lipids may have important consequences on the blood pressure regulation.The Mediterranean-style diet (MD) is characterized by a high consumption of virgin olive oil (VOO) and nuts, which are rich natural sources of oleic (18:1; n-9) and ␣-linolenic (18:3; n-3) acids, respectively. The Prevencion con Dieta Mediterranea (PREDIMED) Study is a large-scale, randomized trial aimed at assessing the effects of a MD enriched with VOO or nuts on primary prevention of cardiovascular disease in patients at high risk for coronary heart disease. The results of the 3-month intervention on the first 772 patients entering the study showed that, compared with a low-fat diet, the MD rich in VOO or nuts reduced systolic blood pressure and serum total cholesterol and triglyceride concentrations and increased serum high-density lipoprotein cholesterol concentration. 7 Although there is evidence indicating that dietary lipids can have a positive effect on cardiovascular risk factors, the mechani...

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“…65 Decreased lipid core microviscosity was also found in membranes isolated from aorta, 66 intestinal brush border, 67,68 and liver of SHR. 31 Moreover, platelets of essential hypertensive patients are characterized by unaltered DPH anisotropy but decreased TMA-DPH anisotropy that correlates negatively with BP.…”

mentioning

confidence: 90%

Abnormalities of membrane function and lipid metabolism in hypertension A review

Zicha

Kuneš

Devynck

1999

American Journal of Hypertension

211

175

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Hypertension, which is characterized by multiple alterations in the structure and function of the cell membrane, is often associated with important metabolic abnormalities including those concerning lipid metabolism. Dyslipidemia accompanying essential hypertension consists of elevated plasma triglycerides, low HDL cholesterol, and increased levels of atherogenic LDL cholesterol particles. The altered membrane microviscosity seen in hypertensive subjects reflects the changes of membrane lipid composition resulting from intensive exchange between circulating and membrane lipids, as well as from abnormal cellular lipid synthesis and metabolism. The changes of membrane microviscosity are known to modulate the activity of proteins involved in ion transport, signal transduction, cell Ca 2؉ handling, intracellular pH regulation, etc. Alterations in plasma or membrane lipids are indeed closely associated with ion transport abnormalities as well as with impaired control of cytosolic Ca 2؉ T here is no doubt that essential hypertension is associated with multiple membrane alterations, including changes in membrane microviscosity, receptor function, signal transduction, ion transport, calcium mobilization, intracellular pH regulation, and so on. Lipids, as an integral part of the cell membrane, play a decisive role in the modulation of the membrane properties mentioned. Specific lipid-lipid and lipid-protein interactions result in a highly dynamic but precisely controlled architecture of membrane components. Major regulators of membrane architecture are membrane potential, intracellular Ca 2ϩ and pH, lipid composition, cell-to-cell contact, and membrane coupling with the cytoskeleton or extracellular matrix. Intermolecular associations in the membrane and at the membrane-cytoskeleton interface are further selectively controlled by specific phosphorylation and dephosphorylation cascades involving both proteins and lipids. This is regulated by the extracellular matrix as well as by the binding of growth factors and hormones to their specific receptors. 1,2The aim of this review is to outline some interrelationships between abnormal lipid metabolism and altered membrane structure or function in human and experimental hypertension, and to evaluate whether abnormal lipid metabolism, which is responsible for a great part of membrane abnormalities found in hypertension, might be an integral part of its pathogenetic mechanisms. BLOOD PRESSUREMultiple metabolic abnormalities often accompany essential hypertension. Decreased high-density lipoproteins (HDL) together with increased plasma levels of low-density (LDL) and very low-density lipoproteins (VLDL), as well as hypertriglyceridemia, hypercholesterolemia, and insulin resistance, were found in many hypertensive patients. 3,4 There is increasing evidence for a genetic basis for the association of hypertension with insulin resistance and dyslipidemia. The genetic locus associated with dyslipidemia accompanying hypertension or diabetes seems to be closely linked to the LDL recept...

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Lipid composition and fluidity in the jejunal brush-border membrane of spontaneously hypertensive rats. Effects on activities of membrane-bound proteins

Cited by 17 publications

References 43 publications

Effects of 2-hydroxyoleic acid on the structural properties of biological and model plasma membranes

Effects of 2-hydroxyoleic acid on the structural properties of biological and model plasma membranes

Mediterranean-Style Diet Effect on the Structural Properties of the Erythrocyte Cell Membrane of Hypertensive Patients

Abnormalities of membrane function and lipid metabolism in hypertension A review

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