Lipid composition-dependent incorporation of multiple membrane proteins into liposomes

Perez, Kátia Regina; Ferreira, Rinaldo Bueno; Thedei, Geraldo; Maggio, Bruno; Ciancaglini, Pietro

doi:10.1016/j.colsurfb.2004.03.015

Cited by 36 publications

(51 citation statements)

References 41 publications

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“…To form unilamellar vesicles with a single concentric bilayer similar to natural membranes, multilamellar vesicles can be sonicated or extruded to nanometric dimensions. Sonication as well as extrusion processes supply the necessary energy to break the different lamellas and reorganize the bilayers into uniform and control-sized vesicles (Camolezi et al 2002;Daghastanli et al 2004;de Lima Santos et al 2005;Ierardi et al 2002;Rigos et al 2008;Simao et al 2015).…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

“…In addition, proteins tend to accommodate among the lipid groups, creating lipid-protein domains and forming the proteoliposomes. By changing the type and proportion of the lipids, incubation time, method used for detergent removal or even the velocity by which the detergent is removed, different vesicular systems can be obtained, varying in the type and quantity of proteins that can be reconstituted (Bolean et al 2010(Bolean et al , 2011Camolezi et al 2002;Daghastanli et al 2004;de Lima Santos et al 2005;Ierardi et al 2002;Rigos et al 2008Rigos et al , 2010Simao et al 2010a), 2015. Anchored membrane proteins are a class of proteins with a covalently bound lipid or alkyl chain; this chain provides a hydrophobic moiety that allows attachment to the membrane and plays specific functions in cells (Yeagle 1993).…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

“…Anchored membrane proteins are a class of proteins with a covalently bound lipid or alkyl chain; this chain provides a hydrophobic moiety that allows attachment to the membrane and plays specific functions in cells (Yeagle 1993). For this class of proteins, the method of incorporation via direct insertion guarantees that the protein is associated with the external leaflet of the liposome's membrane (Bolean et al 2010(Bolean et al , 2011Camolezi et al 2002;Daghastanli et al 2004;de Lima Santos et al 2005;Ierardi et al 2002;Rigos et al 2008Rigos et al , 2010Simao et al 2010aSimao et al , 2015. The incorporation of the protein/enzyme is time-dependent and the incorporation yield depends on the lipid composition used to form the vesicles .…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

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Biophysical aspects of biomineralization

et al. 2017

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membranelimited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition. Phosphatases (orphan phosphatase 1, ectonucleotide pyrophosphatase/ phosphodiesterase 1 and tissue-nonspecific alkaline phosphatase) play a crucial role in controlling the inorganic pyrophosphate/inorganic phosphate ratio that allows MVmediated initiation of mineralization. The lipidic microenvironment can help in the nucleation process of first crystals and also plays a crucial physiological role in the function of MVassociated enzymes and transporters (type III sodiumdependent phosphate transporters, annexins and Na + /K + ATPase). The whole process is mediated and regulated by the action of several molecules and steps, which make the process complex and highly regulated. Liposomes and proteoliposomes, as models of biological membranes, facilitate the understanding of lipid-protein interactions with emphasis on the properties of physicochemical and biochemical processes. In this review, we discuss the use of proteoliposomes as multiple protein carrier systems intended to mimic the various functions of MVs during the initiation and propagation of mineral growth in the course of biomineralization. We focus on studies applying biophysical tools to characterize the biomimetic models in order to gain an understanding of the importance of lipid-protein and lipid-lipid interfaces throughout the process.Keywords Lipid microenvironment . Biomineralization . Matrix vesicles . Liposome . Proteoliposome . Hydroxyapatite Mineralization process and the biogenesis of matrix vesiclesMineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of calcium phosphate. The deposited calcium phosphate is subsequently converted into hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] in specific areas of the extracellular matrix (ECM). Various experiments have revealed the presence of HA crystals both inside and outside collagen fibrils in the ECM (McNally et al. 2012) and also within the

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Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

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Biophysical aspects of biomineralization

et al. 2017

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“…For example, MLVs may be sonicated or passed through a membrane with pores of nanometric dimensions (extrusion method) to form unilamellar vesicles that have properties very similar those of to natural membranes. Both the sonication and extrusion methods provide the energy required to break the various lamellae that initially formed and reorganize the system into uniform bilayers with controlled sizes of vesicles (Camolezi et al 2002;Ierardi et al 2002;Daghastanli et al 2004;Santos et al 2002Santos et al , 2005Rigos et al 2008;dos Santos et al 2009;Bolean et al 2010Bolean et al , 2011Simão et al 2010a, b;Ciancaglini et al 2012).…”

Section: Liposomesmentioning

confidence: 99%

“…Such systems can be used as biomimetic models for studies of interaction with various hydrophobic drugs and membrane proteins, with potential applications in the field of immunology (vaccines and drugs targeting specific cell membranes). Liposomes associated with proteins can be used to study the activity of photosensitive dyes applied during photodynamic therapy (Bolfarini et al 2012;Longo et al 2012;Rocha et al 2012;Bicalho et al 2013;Faria et al 2015), as well for studies related to the ability of proteins to prevent, treat and/or act as nanosensors for various diseases (Daghastanli et al 2004;Santos et al 2006;Zucolotto et al 2007;Migliaccio et al 2008;Colhone et al 2009Colhone et al , 2015Calderon et al 2009a, b;Perinoto et al 2010;Paulovich et al 2011;Barbosa et al 2011;Salay et al 2011;Barros et al 2013;Fotoran et al 2015).…”

Section: Liposomal Systems As Carriers For Bioactive Compoundsmentioning

confidence: 99%

Liposomal systems as carriers for bioactive compounds

et al. 2015

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Since the revolutionary discovery that phospholipids can form closed bilayered structures in aqueous systems, the study of liposomes has become a very interesting area of research. The versatility and amazing biocompatibility of liposomes has resulted in their wide-spread use in many scientific fields, and many of their applications, especially in medicine, have yielded breakthroughs in recent decades. Specifically, their easy preparation and various structural aspects have given rise to broadly usable methodologies to internalize different compounds, with either lipophilic or hydrophilic properties. The study of compounds with potential biotechnological application(s) is generally related to evaluation and risk assessment of the possible cytotoxic or therapeutic effects of the compound under study. In most cases, undesirable sideeffects are associated with an interaction of the liposome with the cell membrane and/or its absorption and subsequent interaction with a cellular biomolecule. Liposomal carrier systems have an unprecedented potential for delivering bioactive substances to specific molecular targets due to their biocompatibility, biodegradability and low toxicity. Liposomes are therefore considered to be an invaluable asset in applied biotechnology studies due to their potential for interaction with both hydrophilic and lipophilic compounds.

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Biomedical Applications of Nanotechnology

Labhasetwar¹,

Leslie‐Pelecky²

2007

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The ability to investigate substances at the molecular level has boosted the search for materials with outstanding properties for use in medicine. The application of these novel materials has generated the new research field of nanobiotechnology, which plays a central role in disease diagnosis, drug design and delivery, and implants. In this review, we provide an overview of the use of metallic and metal oxide nanoparticles, carbon-nanotubes, liposomes, and nanopatterned flat surfaces for specific biomedical applications. The chemical and physical properties of the surface of these materials allow their use in diagnosis, biosensing and bioimaging devices, drug delivery systems, and bone substitute implants. The toxicology of these particles is also discussed in the light of a new field referred to as nanotoxicology that studies the surface effects emerging from nanostructured materials.

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Lipid composition-dependent incorporation of multiple membrane proteins into liposomes

Cited by 36 publications

References 41 publications

Biophysical aspects of biomineralization

Biophysical aspects of biomineralization

Liposomal systems as carriers for bioactive compounds

Biomedical Applications of Nanotechnology

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