Lipid Composition of a Thermotolerant Yeast,<i>Hansenula polymorpha</i>

Wijeyaratne, Sushila Chandrani; Ohta, Kazuyoshi; Chavanich, Surina; Mahamontri, Veravudh; Nilubol, Naline; Hayashida, Shinsaku

doi:10.1080/00021369.1986.10867502

Cited by 12 publications

(13 citation statements)

References 2 publications

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“…In fact, the cultivation of yeast at low temperatures promoted the accumulation of lipids [26]. Although the pLM185 cultures had a tendency to accumulate a large quantity of fatty acids at a lower temperature, the maximum amount of fatty acids was obtained when the yeast was cultured in the presence of 2% methanol at 30°C that may be a result of either the enzymatic activity or the inducer availability under such conditions.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Acetyl-CoA Carboxylase Gene of Mucor rouxii Enhanced Fatty Acid Content in Hansenula polymorpha

2009

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Malonyl-CoA is an essential precursor for fatty acid biosynthesis that is generated from the carboxylation of acetyl-CoA. In this work, a gene coding for acetyl-CoA carboxylase (ACC) was isolated from an oleaginous fungus, Mucor rouxii. According to the amino acid sequence homology and the conserved structural organization of the biotin carboxylase, biotin carboxyl carrier protein, and carboxyl transferase domains, the cloned gene was characterized as a multi-domain ACC1 protein. Interestingly, a 40% increase in the total fatty acid content of the non-oleaginous yeast Hansenula polymorpha was achieved by overexpressing the M. rouxii ACC1. This result demonstrated a significant improvement in the production of fatty acids through genetic modification in this yeast strain.

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Section: Discussionmentioning

confidence: 99%

Overexpression of Acetyl-CoA Carboxylase Gene of Mucor rouxii Enhanced Fatty Acid Content in Hansenula polymorpha

2009

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“…Physiologically, thermotolerance is closely related to the endogenous accumulation of polyols and trehalose in fungi [9-11, 22, 26], and the alteration of cell wall lipid compositions in yeast and bacteria [7,8,23,29]. Such tolerance can be acquired by careful manipulation of the osmotic pressure of culture media [9,21,28] or by supplying materials as substrates for the induction of thermotolerance [3,14,24,25,27].…”

Section: Introductionmentioning

confidence: 99%

Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture

Roh

2010

J Ind Microbiol Biotechnol

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Low thermotolerance of entomopathogenic fungi is a major impediment to long-term storage and effective application of these biopesticides under seasonal high temperatures. The effects of high temperatures on the viability of an entomopathogenic fungus, Isaria fumosorosea SFP-198 (KCTC 0499BP), produced on different substrates amended with various additives were explored. Ground corn was found to be superior in producing the most thermotolerant conidia compared to yellow soybean, red kidney bean, and rice in a polyethylene bag production system. Using ground corn mixed with corn oil as a substrate resulted in only 7% reduction in germination compared to ground corn alone (67% reduction) after exposure of conidia to 50 degrees C for 2 h. Corn oil as an additive for ground corn was followed by inorganic salts (KCl and NaCl), carbohydrates (sucrose and dextrin), a sugar alcohol (sorbitol), and plant oils (soybean oil and cotton seed oil) in ability to improve conidial thermotolerance. Unsaturated fatty acids, such as linoleic acid and oleic acid, the main components of corn oil, served as effective additives for conidial thermotolerance in a dosage-dependent manner, possibly explaining the improvement by corn oil. This finding suggests that the corn-corn oil mixture can be used to produce highly thermotolerant SFP-198 conidia and provides the relation of unsaturated fatty acids as substrates with conidial thermotolerance.

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“…In contrast, the methylotrophic yeast Hansenula polymorpha has the capability to produce PUFAs such as linoleic acid (C18:2-Δ 9,12) and linolenic acid (C18:3-Δ 9,12,15) in addition to MUFAs. Therefore, this yeast has been recently considered to be an appropriate model organism for studying the mechanism underlying fatty acid biosynthesis (Wijeyaratne et al, 1986;Anamnart et al, 1998;Lu et al, 2000;Kaneko et al, 2003;Laoteng et al, 2005). In H. polymorpha, two genes involved in fatty acid biosynthesis, namely, the fatty acid synthase β-polypeptide gene (FAS1) and the fatty acid Δ9-desaturase gene (H-OLE1), have been cloned and characterized (Anamnart et al, 1997;Lu et al, 2000;Kaneko et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha

Prasitchoke

Kaneko

Bamba

et al. 2007

Gene

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Lipid Composition of a Thermotolerant Yeast,Hansenula polymorpha

Cited by 12 publications

References 2 publications

Overexpression of Acetyl-CoA Carboxylase Gene of Mucor rouxii Enhanced Fatty Acid Content in Hansenula polymorpha

Overexpression of Acetyl-CoA Carboxylase Gene of Mucor rouxii Enhanced Fatty Acid Content in Hansenula polymorpha

Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture

Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha

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