Lipid composition of cells and low-density lipoproteins in blood serum of human and some vertebrate species

Lizenko, M. V.; Regerand, T. I.; Bakhirev, A. M.; Lizenko, E. I.

doi:10.1134/s0022093011050045

Cited by 8 publications

(9 citation statements)

References 4 publications

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“…Most biological membranes carry a net negative charge 29 and are, due to attractive electrostatic forces, expected to interact more strongly with the highly cationic PAMAM dendrimers than the neutral PC bilayer. In Fig.…”

Section: Effect Of Membrane Chargementioning

confidence: 99%

Induced dye leakage by PAMAM G6 does not imply dendrimer entry into vesicle lumen

et al. 2012

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Dendrimers are polymers with unique properties that make them promising in a variety of applications such as potential drug and gene delivery systems. Polyamidoamine (PAMAM) dendrimers, in particular, have been widely investigated since they enter rapidly into cells. The entry mechanism, however, is still not yet fully clarified as both passive and active uptake have been proposed. In this work we focus on understanding passive uptake, for which simple cell model systems are used in order to ensure that only dendrimer-lipid interactions are probed. We developed protocols for investigating independently the effect of the dendrimer on lipid bilayer integrity, in terms of permeability of small dyes and effective dendrimer translocation. This was achieved by the use of membrane labeled giant unilamellar vesicles (GUVs) either containing Alexa 488 hydrazide in the vesicle lumen or FITC-labeled PAMAM G6 dendrimers. Vesicle integrity and dendrimer-membrane binding were then assessed by fluorescence microscopy. The importance of membrane fluidity and charge was investigated using GUVs composed of various lipid compositions. A quartz crystal microbalance with dissipation was used to probe the effect of dendrimers on the rigidity of vesicle layers. The results indicate that PAMAM dendrimers can locally alter the membrane properties. An increased bilayer permeability towards soluble small dyes but no effective translocation, where PAMAM dendrimers could dissociate from the lipid membrane into the vesicle lumen, was observed. To our knowledge this is the first time it is shown that PAMAM G6 dendrimer does not effectively translocate the lipid bilayer although it readily interacts with the model membrane, regardless of lipid membrane properties. However, bilayer charge and fluidity modulate the dendrimer interaction in agreement with previous reports. The results clearly highlight the importance of the choice of the model system when investigating nanoparticles interaction with lipid membranes.

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Section: Effect Of Membrane Chargementioning

confidence: 99%

Induced dye leakage by PAMAM G6 does not imply dendrimer entry into vesicle lumen

et al. 2012

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“…It is known that an increased level of total cholesterol and its atherogenic fractions, LDL and VLDL, as well as the decreased content of HDL can be predicting for the development of atherosclerotic processes [19]. It was also shown that LDL and VLDL lipid composition directly influences blood formula and lipid ratio in the blood cells [20,21]. For females of the native ethnos, nutrition may be crucial.…”

Section: Discussionmentioning

confidence: 99%

Adaptive reactions of lipid metabolism in indigenous and non-indigenous female individuals of tofalarian population living under extreme environmental conditions

Kolesnikova

Darenskaya

Grebenkina

et al. 2014

J Evol Biochem Phys

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Age-dependent peculiarities of lipid metabolism and antioxidant defense mechanisms of lipid peroxidation are examined in indigenous versus non-indigenous female individuals of the Tofalaria's population. The results indicate the specificity of lipid metabolism changes not only in response to different length of exposure to climatic factors but in different ethnic groups. Thus, in native Tofalarian girls, unlike incomers, there occurs an activation of adaptive-compensatory processes as evidenced by a statistically significant decrease in the blood atherogenic fractions and overall activation of the antioxidant defense system. Although with age the reactions of lipid peroxidation change in both ethnic groups, in non-indigenous females these changes are more pronounced.

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“…To study the role of distinct lipid composition, relevant for mammalian cell membranes 15,16 S1, in which for each lipid composition vesicles were diluted in buffer and observed at the same conditions. This control showed that GUVs were stable, circular structures, whose phase contrast intensity was kept unchanged throughout the observation period regardless of the lipid composition.…”

Section: Quercetin Induces Lipid Domain-dependent Leakagementioning

confidence: 99%

Quercetin Induces Lipid Domain-Dependent Permeability

Leite¹,

Martins²,

Cabrera³

2021

Preprint

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In this work, we present our results on quercetin interaction with distinct model membranes exploring the importance of lipid phases, ld, ld/lo and ld+lo+so, to the action of this flavonoid in bilayers and possibly contributing to clarifying some controversial aspects related to quercetin multiple activities. We found out that quercetin is able to increase membrane permeability in a manner dependent on the presence and characteristics of lipid domains. In the presence of sphingomyelin, we found the greatest increase in mean membrane permeability (at least 10 times higher than the other lipid compositions). We also observed the presence of micrometric domains whose shape and size were disturbed by the action of quercetin. The presence of cholesterol increased membrane rigidity. This effect was enhanced with the presence of quercetin, but for chol-sphingomyelin combination, the bilayers became more flaccid at low quercetin/lipid proportions (< 1/5) and moderately rigid at proportions of the 1/1 order. The affinity parameters were higher for the most homogeneous systems and with larger areas and extensions of disordered liquid phase than for those systems of higher heterogeneity.

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Lipid composition of cells and low-density lipoproteins in blood serum of human and some vertebrate species

Cited by 8 publications

References 4 publications

Induced dye leakage by PAMAM G6 does not imply dendrimer entry into vesicle lumen

Induced dye leakage by PAMAM G6 does not imply dendrimer entry into vesicle lumen

Adaptive reactions of lipid metabolism in indigenous and non-indigenous female individuals of tofalarian population living under extreme environmental conditions

Quercetin Induces Lipid Domain-Dependent Permeability

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