Lipid composition of Halobacterium lacusprofundi

Abstract

The recently described aerobic, extremely halophihc archaeobaetenum, Halnbacterlum lacusprofandt was subJected to hpld analysis so that comparisons could be made between erastmg lipid data and that of the new Isolate. This mvestlgaUon showed that the major respwatory hpoqumones present were MK-8 and MK-8(VIII-H2), a feature found m other members of the family Halobactenaceae. The polar hptds comprised the dtether dertvatwes of phosphatidyl glycerol, phosphaUdyl glycerophosphate, phosphattdyl glycerosulphate, a dlglycosyl d]ether and its sulphatcd dertvaUve The data presented shows that Halobactenum lacusprofundl ts related to Hb saecharovorum and Hb. sodomense, and is m agreement with phylogenetic data 2.1NTRODUC~ONThe taxonomy of the famdy Halobactenaceae has undergone extensive revision within the last decade [l] A contributing factor to tlus has been Cor,espondence to B J "Iindall, DSM-Deutsche Sammlung yon Mikroorgamsmen und Zellkulturen GmbH. Mascheroder Weg lb. I)-3300 Braunschwelg, F R O the thscovery of new isolates which do not conform to the older concepts of halobactenal dlver-sRy. and to the use of molecular biologmal approaches combined with conventional btochenucal and physiological studies. While the present generic diversity of the farmly Halobacter:aeceae Is supported to some degree by 16S rRNA data the major evidence for the current dtvlsions comes from a combination of nucletc acid hybridisatton and polar hpld analyses [2][3][4][5]. In fact, R has been possible to show that, by using polar hpid analysis alone, the fanuly Halobactertaceae may be divided mto 9 different groups, of which 6 eor'~spond to the currently recogmsed genera [1,2,4,6]. That the remaining three groups have not been allocated separate genene status appears to rest on cert~un taxononuc formalities. In view of the fact that it is possible to accurately and reliably assign a new isolate to one of the existing genera within the fan'aly Halobactenaceae the newly descnbed speoes Halobactertulm lacusprofuadt [7] was subjeered to polar hpid analysis to deterrmne m which of the polar lipid groups tt belongs. 16S rRNA analysis showed that tins species was related to Halobactertum saccharovorum and Halobacterium sodomense Consequently the hpid c.ompositmn of antheutlc type strains of the latter two species were cempared with the hpld composltmn of HalobacterJum lacusprofundt, m order to proxqde 0378-1097/89/$03 50

Virgibacillus flavescens sp. nov., isolated from marine sediment

“…The predominant menaquinone of strain S1-20 T was MK-7 (99 %) with minor amounts of MK-6 (1 %) also present, which is in agreement with the genus description (Heyrman et al, 2003;Niederberger et al, 2009;Lee et al, 2012). Polar lipid profiles were analysed according to Tindall (1990a, b). The polar lipid profile of strain S1-20 T contained diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids, one unknown aminophospholipid and an aminolipid (Fig.…”

Carnimonas nigrificans gen. nov., sp. nov., a bacterial causative agent for black spot formation on cured meat products

“…Bacto OF basal medium (Difco) was used for the determination of acid production from different carbohydrates added to a final concentration of 1 Yo (w/v). Respiratory lipochinones were extracted according to Tindall (1990) and analysed by HPLC as described by Franzmann & Tindall(l990). The cellular fatty acid composition of strain CTCBSIT was determined by GC after the preparation of whole-cell methanolysates (Minnikin et al, 1977).…”

Sulfurospirillum arcachonense sp. nov., a New Microaerophilic Sulfur-Reducing Bacterium

“…Respiratory lipoquinones and polar lipids were extracted from 100 mg of freeze-dried cell material by using the two-stage method described by Tindall (44,45). Polar lipids were extracted by adjusting a methanol-0.3% aqueous NaCl phase to give a chloroform-methanol-0.3% aqueous NaCl mixture (1:2:0.8, vol/vol/vol).…”