Lipid composition of lymphocyte plasma membrane from pig mesenteric lymph node

Levis, Gabriel M.; Evangelatos, Gregory P.; Crumpton, Michaël J.

doi:10.1042/bj1560103

Cited by 26 publications

(14 citation statements)

References 37 publications

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“…Gangliosides (SP0601) constitute a subgroup of acidic glycosphingolipids (SP06) with one or more terminal sialic acids. The total ganglioside content of the plasma membrane (PM) can reach up to 1% -2%, with individual gangliosides in the range of 0.01% -0.1% (Levis et al 1976;Ortegren et al 2004;Sonnino et al 2006). The glycosphingolipids are enriched in the apical domain of polarized cells (Crespo et al 2008), and they are especially abundant in neurons (Sonnino et al 2006).…”

Section: Glycosphingolipidsmentioning

confidence: 99%

Lipid-Mediated Endocytosis

Ewers¹,

Helenius²

2011

Cold Spring Harbor Perspectives in Biology

164

146

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Section: Glycosphingolipidsmentioning

confidence: 99%

Lipid-Mediated Endocytosis

Ewers¹,

Helenius²

2011

Cold Spring Harbor Perspectives in Biology

164

146

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Section: Discussionmentioning

confidence: 88%

“…Only traces of this small ganglioside are found in normal human lymphocytes, but the lymphoblastoid cells BRI-8 contain considerable amounts, primarily concentrated in their plasma membranes, where a 10-fold increase enrichment has been found [6]. Plasma membranes from pig mesenteric lymph nodes are also enriched in gangliosides [13]. Since lymphocytes are transformed to lymphoblasts after stimulation with lectins, it might tie suggested that an active biosynthesis of II'NeuAc-LacCer by these cells, in accordance with evidence mentioned above, is needed to satisfy requirements for new membrane formation.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore variations in glycosphingolipid composition of lymphocytes at various stages of' growth has been reported [6,17,18].…”

mentioning

confidence: 99%

“…The study of the effect of lymphocyte stimulation on glycosphingolipid metabolism is of considerable interest in connection with the important functions ascribed to these cell-surface components [6,13] in relation to their role played in cell growth [14], in receptors [15] and ion-binding mechanisms [16].…”

mentioning

confidence: 99%

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Studies on Glycosphingolipid Biosynthesis by Lectin-Stimulated Human Lymphocytes

et al. 1980

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1. Human peripheral lymphocytes, normal and stimulated by phytohemagglutinin or concanavalin A, were investigated with respect to their ability to biosynthesize neutral and acidic glycosphingolipids from D‐[U‐14C]glucose, D‐[U‐14C]galactose and D‐[U14C]glucosamine as precursors. 2. Galactose and glucosamie are taken up selectively, in the presence of excessive glucose concentrations. Labeling of total neutral glycosphingolipids from D‐[U‐14C]galactose in normal cells decreases after the first 6 h while in stimulated cells there is a fourfold and a sevenfold increase after 6 and 12 h of incubation respectively. Under similar conditions stimulation with concanavalin A gives a fourfold increase after 12 h of incubation. 3. Analysis of individual glycosphingolipids biosynthesized from [U‐14C]galactose shows that lactosylceramide is the major radioactive neutral glycosphingolipid and that stimulation by phytohemagglutinin yields an almost sevenfold and 13‐fold increase in the radioactivity incorporated within 6 and 12 h of incubation respectively. Glucosylceramide shows about an eightfold increase, globotetraosylceramide a threefold increase and globotriaosylceramide a fourfold increase. The rate of incorporation into glucosylceramide of stimulated lymphocytes declines after 6 h of incubation, accompanied by a concomitant increase of incorporation into lactosylceramide. 4. At 12 h of incubation the ratios of radioactivities incorporated into neutral glycosphingolipids of phytohemagglutinin‐stimulated cells compared to normal cells were 1.0 for D‐[U‐14C]glucose 7.0 for D‐[U‐14C]galactose and 2.5 for D‐[U‐14C]glucosamine. Respective ratios for lactosylceramide are 1.0 for [U‐14C]glucose and 13.0 for [U‐14C]galactose. These differences did not arise from changes of the uptake of the glycosyl precursor by the cell due to stimulation. 5. Incorporation of D‐[U‐14C]galactose into II3‐N‐acetylneuraminosyllactosylceramide by cells stimulated by phytohemagglutinin and concanavalin A is enhanced 14‐times and 15‐times respectively. With [U‐14C]glucosamine as precursor, this increase in the labeling is much more impressive, 80‐fold after 12 h of incubation by the phytohemagglutinin‐stimulated lymphocytes. Neuraminidase treatment and gas radiochromatographic analysis of the labeled compound derived from [U‐14C]‐galactose as precursor indicated that 89% of the radioactivity was incorporated into the glucosyl and galactosyl moieties, in a ratio 1:1. With [U‐14C]glucosamine as precursor, a selective labeling of the sialyl moiety of the II3‐N‐acetylneuraminosyllactosylceramide was indicated. 6. The pattern of complex gangliosides (more complex than II3‐N‐acetylneuraminosyllactosylceramide) which are biosynthesized after phytohemagglutinin stimulation of the cells, show no significant differences when compared to the patterns obtained from normal cells.

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Isolation and partial characterization of the neutral glycosphingolipids and gangliosides of the human heart

Levis¹,

Karli²,

Moulopoulos³

1979

Lipids

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The glycosphingolipids (GSL) of the human heart muscle have been isolated from total lipids by column and thin layer chromatography and their sugars and fatty acids analyzed by gas liquid chromatography. Hearts from traffic victims were obtained at autopsy between 12 and 16 hr after death and dissected into parts (left and right ventricular walls, intraventricular septum and papillary muscle). The neutral GSL content for those parts of the hearts of two males aged 22 and one female aged 14 ranged from about 90 to 160 nmoles/g wet weight. Trihexosyl ceramide and globoside were the most abundant neutral GSL. Total ganglioside content was about 50 nmoles/g wet weight, and the most abundant gangliosides were partially characterized as GM3 and GM1; other mono-, di- and trisialogangliosides were also present. Differences in the content and composition of neutral GSL and gangliosides between the heart and other human tissues are discussed. It is concluded that the patterns of these two GSL fractions of the heart are more complex than those of the extraneural human tissues.

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Lipid composition of lymphocyte plasma membrane from pig mesenteric lymph node

Cited by 26 publications

References 37 publications

Lipid-Mediated Endocytosis

Lipid-Mediated Endocytosis

Studies on Glycosphingolipid Biosynthesis by Lectin-Stimulated Human Lymphocytes

Isolation and partial characterization of the neutral glycosphingolipids and gangliosides of the human heart

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