Lipid Composition of Paracoccidioides brasiliensis: Possible Correlation with Virulence of Different Strains

Manocha, M. S.; San-Blas, Gioconda; Centeno, Sonia

doi:10.1099/00221287-117-1-147

Cited by 57 publications

(47 citation statements)

References 7 publications

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“…The total carbohydrate content was estimated by the phenol-sulphuric acid method (Dubois et al, 1956). For the lipids (Manocha et al, 1980), the fatty acids content on the hydrophobic portion of the biosurfactant was identified by converting it to methyl esters (Durham and Kloos, 1978). The methyl esters of fatty acids were suspended in n-hexane and analyzed by gas chromatography.…”

Section: Characterization Of the Biosurfactantmentioning

confidence: 99%

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Lima¹,

Andrade²,

RodrÃguez³

et al. 2017

Afr. J. Microbiol. Res.

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The world market for biosurfactants has grown gradually. However, the lack of competitiveness with chemical surfactants due to high cost of production remains a concern. Considering the need to reduce the costs of production, the aim of this work was to study the production and structural characterization of a biosurfactant produced by a strain of yeast Candida glabrata UCP 1556. The lowcost medium containing agro-industrial wastes whey 40% (v/v) and 20% (v/v) corn steep liquor were used as substrates in submerged fermentation. Biosurfactant production was detected by surface tension, oil displacement test and Critical Micelle Concentration (CMC). The structural characterization was performed by Fourier transform infrared (FT-IR), gas chromatography-mass spectrometry (GC-MS) and ionic profile. The stability of emulsions and potential in reducing the viscosity also were investigated. The results showed that the biosurfactant reduced the surface tension to 28.8 mN/m with CMC of 2% and showed anionic profile. Additionally, the biosurfactant formed stable emulsions at temperature (0 to 120°C), pH (2 to 12) and NaCl (2 to 12%), reduced the viscosity of soybean oil (

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Section: Characterization Of the Biosurfactantmentioning

confidence: 99%

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Lima¹,

Andrade²,

RodrÃguez³

et al. 2017

Afr. J. Microbiol. Res.

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“…Yeast cells in the exponential phase (31) were collected aseptically with a platinum loop and resuspended in Erlenmeyer flasks modified by the insertion of a lateral tube containing sterile 0.85% saline solution for readings with a photocolorimeter. After homogenization by vortexing, transmittance was measured at 520 nm and adjusted to 69 to 70%.…”

mentioning

confidence: 99%

Effects of Amphotericin B and Three Azole Derivatives on the Lipids of Yeast Cells of Paracoccidioides brasiliensis

Hahn

Hamdan

2000

Antimicrob Agents Chemother

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Yeast cells of five different strains of Paracoccidioides brasiliensis were obtained for partial analysis of lipid composition, and sterol content was determined quantitatively and qualitatively. The determinations were conducted with cells cultured in the presence and absence of amphotericin B and azole derivatives at levels below the MIC.Paracoccidioidomycosis is a chronic granulomatous disease with a diversity of clinical manifestations, and special emphasis has been placed on the pulmonary and mucocutaneous forms (29,42). The disease is limited to Latin American countries, and it is considered the most prevalent systemic mycosis in Latin America (27)(28)(29)38).The dimorphic fungus Paracoccidioides brasiliensis is the single etiologic agent of paracoccidioidomycosis, which is acquired by inhalation of spores of the mycelial phase of the fungus (3,38,43,45).In the present study we assessed the effects of amphotericin B, ketoconazole, itraconazole, and fluconazole on the sterols of five different P. brasiliensis strains in order to better evaluate the in vitro efficacy of these drugs. The MIC of each drug and the effects of the compounds on total sterol content were determined.Fungal strains and culture conditions. Five P. brasiliensis strains were studied. Of these, JT-1, a human isolate, corresponded to the reference strain ATCC 90659. Three other strains, called Pinguim, SN, and 18, were from the fungal collection of the Faculty of Medicine of the Universidade de São Paulo and were a kind gift from C. Fava-Netto (Universidade de São Paulo) and Patrícia Cisalpino (Instituto de Ciencias Biológicas, Universidade Federal de Minas Gerais). The environmental strain Pinguim was isolated from the feces of a penguin in the Antarctic and characterized as P. brasiliensis in mycologic and immunochemical studies (15). The human isolate RAJ-2 was a kind gift from Tomoko Tadano and originated from the Clinical Pathology Laboratory of the Julio Müller University Hospital, Cuiabá-MT, where it had been obtained from the sputum of a patient at the Julio Müller University Hospital.All strains were maintained on solid Fava-Netto medium at 22 to 28°C with monthly subculture and converted to the yeastlike phase by incubation at 35°C. The full transition of the forms was confirmed by the observation of the macroscopic and microscopic aspects of the colonies obtained.For the study of lipid composition and of the effects of amphotericin B and the three azoles, the microorganisms were adapted to solid McVeigh Morton (MVM) medium, modified as described previously (39).Antifungal agents. All strains were tested in the presence of amphotericin B, ketoconazole, fluconazole, and itraconazole. Amphotericin B was obtained from Sigma Chemical Company in pure form and was stored at 4°C in a dark glass bottle. The azole derivatives ketoconazole and itraconazole were kindly provided by Janssen in the form of pure salts and were stored at room temperature.The studies involving fluconazole were carried out using a commercial preparation (Fluconal...

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“…Carbohydrates were determined by the phenol-sulphuric acid method, using Dglucose as a standard (Hanson and Phillips, 1981). The lipid composition of the crude bioemulsifier was determined according to Manocha et al (1980). Figure 1 shows the biomass concentration, pH and emulsification index of Candida glabrata cultivation in mineral medium containing 7.5% of cotton seed oil plus 5% of glucose.…”

Section: Methodsmentioning

confidence: 99%

Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002

Sarubbo¹,

Luna²,

Campos-Takaki³

2006

Electron. J. Biotechnol.

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Evaluation of both tenso-active and emulsifying activities indicated that a biosurfactant was produced by the newly isolated and promising strain Candida glabrata isolated from mangrove sediments. The extracellular water-soluble emulsifying agent was isolated and identified as a heteropolymer. The maximum of bioemulsifier production was observed when the strain was grown on soluble and insoluble substrates cotton seed oil plus glucose, reaching values *Corresponding author of 10.0 g/l after 144 hrs at 200 rpm. The cell-free culture broth containing the examined agent lowered the surface tension of the medium to 31 mN/m. Stable and compact emulsions with emulsifying activity of 75% of cotton seed oil were detected. The emulsification capacity remained practically unaltered within a wide pH (2-12), temperature (4-80ºC) ranges and under NaCl concentrations up to 10%.

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Lipid Composition of Paracoccidioides brasiliensis: Possible Correlation with Virulence of Different Strains

Cited by 57 publications

References 7 publications

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Effects of Amphotericin B and Three Azole Derivatives on the Lipids of Yeast Cells of Paracoccidioides brasiliensis

Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002

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