Lipid composition of rat liver cell membranes

Skipski, Vladimir P.; Barclay, Marion; Archibald, F. M.; Terebus-Kekish, Olga; Reichman, Eleanore S.; Good, James J.

doi:10.1016/0024-3205(65)90213-4

Cited by 74 publications

(34 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…$ Emmelot et al . (1964) ; Skipski et al . (1965) ; Takeuchi and Terayama (1965) ; Ashworth and Green (1966) ; Coleman and Finean (1966) ; Coleman et al .…”

Section: Purity Of the Plasma Membranesmentioning

confidence: 99%

PLASMA AND PHAGOSOME MEMBRANES OF ACANTHAMOEBA CASTELLANII

Ulsamer

Wright

Wetzel

et al. 1971

The Journal of Cell Biology

116

View full text Add to dashboard Cite

Plasma membranes were isolated from the ameba Acanthamoeba castellanii by low-speed velocity centrifugation followed by equilibrium centrifugation in a sucrose gradient . The isolated membranes had a high ratio of sterol to phospholipid (0 .98 moles/mole) and of phospholipid to protein (0.43 mg/mg) . The plasma membranes had very low concentrations of DNA, RNA, lipid inositol, and glycerides . Glycolipids and glycoproteins were enriched in the plasma membranes relative to their concentrations in the whole cell . The plasma membranes were also judged to be of high purity by the absence, or very low level, of enzymatic activities considered to be indicative of other cell membranes, and by electron microscope examination . Alkaline phosphatase and 5'-nucleotidase activities were enriched in the plasma membranes 13-fold relative to the whole homogenate and had higher specific activities in the plasma membranes than in any other cell fractions . A Mg++ adenosine triphosphatase (ATPase) was enriched sixfold in the plasma membranes relative to the whole homogenate . The phospholipids of the plasma membranes contained more phosphatidylethanolamine and phosphatidylserine and less phosphatidylcholine than did the phospholipids of the whole cells . There were differences in the fatty acid compositions of corresponding phospholipids in the plasma membranes and whole cells but no difference in the ratios of total saturated to unsaturated fatty acids . The membranes of phagosomes isolated from amebae that had ingested polystyrene latex had essentially the same phospholipid, sterol, and enzymatic composition as plasma membranes.

show abstract

“…$ Emmelot et al . (1964) ; Skipski et al . (1965) ; Takeuchi and Terayama (1965) ; Ashworth and Green (1966) ; Coleman and Finean (1966) ; Coleman et al .…”

Section: Purity Of the Plasma Membranesmentioning

confidence: 99%

PLASMA AND PHAGOSOME MEMBRANES OF ACANTHAMOEBA CASTELLANII

Ulsamer

Wright

Wetzel

et al. 1971

The Journal of Cell Biology

116

View full text Add to dashboard Cite

show abstract

“…The single most popular tissue is rat liver (Emmelot and Bos, 1962;Davidson et al, 1963;Tria and Barnabei, 1963;Skipski et al, 1965;Takeuchi and Terayama, 1965;Ashworth and Green, 1966;Lansing et al, 1967;Lieberman et al, 1967;Song and Bodansky, 1967;Anderson et al, 1968;Emmelot et al, 1968;Dod and Gray, 1968;Graham et al, 1968;Neville, 1960Neville, , 1968Stein and Stein, 1968;Berman et al, 1969;Erecinska et al, 1969;Evans, 1969;Wattiaux-de Coninck and Wattiaux, 1969;Weaver and Boyle, 1969;E1-Aaser et al, 1966E1-Aaser et al, , 1970Henning et al, 1970;Hinton et al, 1970;Ray, 1970;Skidmore and Trams, 1970;Touster et al, 1970;Barancik and Lieberman, 1971;Franklin and Trams, 1971;Wright and Green, 1971). Plasma membranes have also been isolated from the livers of cows (Fleischer and Fleischer, 1969), pigs (Lutz and Frimmer, 1970), mice Evans, 1970), guinea pigs Finean, 1966, 1967).…”

Section: Choice Of Tissuesmentioning

confidence: 99%

Plasma Membranes of Mammalian Cells

DePierre

Karnovsky

1973

The Journal of Cell Biology

429

View full text Add to dashboard Cite

“…This change consisted of an increase in the cell outer membrane and its derivatives and a decrease in rough endoplasmic reticulum. Since of all the cellular membranes the plasma membrane was found to be the richest in sphingomyelin (42,43), it seems that it might be the site of accumulation of sphingomyelin with age. A similar interpretation was proposed also by Portman, Alexander, and Maruffo (44), who were able to isolate from monkey aortic homogenates a vesicular fraction, rich in sphingomyelin, which was probably derived from the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

Phospholipases in arterial tissue

Eisenberg¹,

Stein²,

Stein³

1969

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T The role of phospholipases in the regulation of the changing phospholipid composition of normal human aortae with age was studied. Portions of grossly and histologically lesion-free ascending aortae from 16 females and 29 males obtained at autopsy, were analyzed for deoxyribonucleic acid (DNA), phospholipid, and cholesterol content and phospholipid composition. Enzymic activity toward four substrates, lecithin (LE), phosphatidyl ethanolamine, lysolecithin, and sphingomyelin (SP), was determined on portions of the same homogenate. By regression analysis for correlation between all determinations and age the following results were obtained: (a) total phospholipids and choleserol increased linearly with age; (b) the increase in sphingomyelin accounted for about 70% of the phospholipid increment; (c) hydrolysis of lecithin and phosphatidyl ethanolamine increased markedly with age, that of lysolecithin only moderately; (d) hydrolysis of sphingomyelin decreased with age; and (e) an inverse relation between the SP/LE ratio and age and sphingomyelinase/lecithinase activity and age was obtained. These results were interpreted to indicate that a causal relation exists between the fall in sphingomyelinase activity, both absolute and relative to lecithinase activity, and the accumulation of sphingomyelin with age.

show abstract

Lipid composition of rat liver cell membranes

Cited by 74 publications

References 11 publications

PLASMA AND PHAGOSOME MEMBRANES OF ACANTHAMOEBA CASTELLANII

PLASMA AND PHAGOSOME MEMBRANES OF ACANTHAMOEBA CASTELLANII

Plasma Membranes of Mammalian Cells

Phospholipases in arterial tissue

Contact Info

Product

Resources

About