Lipid Composition of Sonchus Yellow Net Virus

Selstam, Eva; Jackson, Andrew O.

doi:10.1099/0022-1317-64-7-1607

Cited by 13 publications

(3 citation statements)

References 34 publications

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“…Triterpenoids are the building blocks for sterols, which in algae are derived from the MVA pathway (Disch et al, 1998). These molecules constitute parts of cellular membranes (Hartmann, 1998), including viral envelopes (Selstam and Jackson, 1983). Therefore, the reduction in sterol abundance during viral infection can be a result of its consumption by newly produced viruses.…”

Section: Discussionmentioning

confidence: 99%

Rewiring Host Lipid Metabolism by Large Viruses Determines the Fate of Emiliania huxleyi, a Bloom-Forming Alga in the Ocean

et al. 2014

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Marine viruses are major ecological and evolutionary drivers of microbial food webs regulating the fate of carbon in the ocean. We combined transcriptomic and metabolomic analyses to explore the cellular pathways mediating the interaction between the bloom-forming coccolithophore Emiliania huxleyi and its specific coccolithoviruses (E. huxleyi virus [EhV]). We show that EhV induces profound transcriptome remodeling targeted toward fatty acid synthesis to support viral assembly. A metabolic shift toward production of viral-derived sphingolipids was detected during infection and coincided with downregulation of host de novo sphingolipid genes and induction of the viral-encoded homologous pathway. The depletion of host-specific sterols during lytic infection and their detection in purified virions revealed their novel role in viral life cycle. We identify an essential function of the mevalonate-isoprenoid branch of sterol biosynthesis during infection and propose its downregulation as an antiviral mechanism. We demonstrate how viral replication depends on the hijacking of host lipid metabolism during the chemical "arms race" in the ocean.

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Section: Discussionmentioning

confidence: 99%

Rewiring Host Lipid Metabolism by Large Viruses Determines the Fate of Emiliania huxleyi, a Bloom-Forming Alga in the Ocean

et al. 2014

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“…After we determined the basic physiochemical properties of SYNV ( 17), more detailed analyses of SYNV structural proteins (18) and lipids (19) were conducted for comparisons with other rhabdoviruses. To determine whether SYNV is a negative-strand virus, I initiated RNA-dependent RNA polymerase studies similar to those reported previously for LNYV by Richard Francki but was unable to confirm polymerase activity in purified SYNV or PYDV.…”

Section: On My Own At Purduementioning

confidence: 99%

Reflections on a Career in Plant Virology: A Chip Floating on a Stream

Jackson

2021

Annu. Rev. Virol.

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At the time I entered college and for a few years afterward, I had very few concrete goals. Hence, my progress was more a matter of luck than planning and was somewhat analogous to a small wood chip floating down a slow stream, bumping into various objects tossed and turned hither and thither, all the while being surrounded by larger and more appealing chips. I have been extremely lucky to have been associated with numerous helpful and knowledgeable mentors, colleagues, postdocs, students, and coworkers whose advice had major impacts on my life. Therefore, throughout this article, I have attempted to acknowledge central individuals who contributed to my progress in academia and to highlight the positive bumps to my chip on the steam that affected the directions of my career. Expected final online publication date for the Annual Review of Virology, Volume 8 is September 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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“…Sonchus yellow net virus (SYNV), a plant rhabdovirus, has bacilliform particles (Jackson andChristie, 1977, 1979) which consist of a distinctive nucleocapsid core and an envelope (Selstam and Jackson, 1983). Physicochemical studies of the virus and analysis of RNAs from infected plants reveal that the genome consists of a minus sense RNA approximately 13,000 nucleotides (nt) long (Milner and Jackson, 1979;Rezaian et a/., 1983).…”

Section: Introductionmentioning

confidence: 99%

Structure of the glycoprotein gene of sonchus yellow net virus, a plant rhabdovirus

et al. 1991

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The nucleotide sequence of the glycoprotein (G) gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, was determined from viral genomic and mRNA cDNA clones. The G cistron is 2045 nucleotides (nt) long and the G protein mRNA open reading frame (ORF), as determined from the cDNA sequence, contains 1896 nt and encodes a protein of 632 amino acids. Immunoblots with antibodies elecited against the purified glycoprotein from virus particles reacted with a fusion protein produced in Escherichia coli, indicating that the cloned ORF encodes the G protein. The 5' end of the G protein mRNA corresponds to nt 5111, relative to the 3' end of the viral (minus sense) genome, as determined by primer extension from mRNA isolated from infected plants, and extends to nt position 7155 on the genomic RNA. A 34-nt untranslated 5' leader sequence and a 115-nt untranslated 3' end flank the ORF on the mRNA. The gene junctions on either side of the G gene on the genomic RNA are identical to those previously described for other SYNV genes and are similar to sequences separating genes of animal rhabdoviruses. The predicted molecular weight of the G protein is 70,215 Da, a value less than the 77,000 Da estimated for the glycosylated G protein from virus particles. The deduced amino acid sequence of the SYNV G protein shares little direct relatedness with the G proteins of other rhabdoviruses, but appears to contain a similar signal sequence, a transmembrane anchor domain, and glycosylation signals. In addition, the SYNV G protein contains a putative nuclear targeting site near the carboxy terminus, which may be involved in transit to the nuclear membrane prior to morphogenesis.

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Lipid Composition of Sonchus Yellow Net Virus

Cited by 13 publications

References 34 publications

Rewiring Host Lipid Metabolism by Large Viruses Determines the Fate of Emiliania huxleyi, a Bloom-Forming Alga in the Ocean

Rewiring Host Lipid Metabolism by Large Viruses Determines the Fate of Emiliania huxleyi, a Bloom-Forming Alga in the Ocean

Reflections on a Career in Plant Virology: A Chip Floating on a Stream

Structure of the glycoprotein gene of sonchus yellow net virus, a plant rhabdovirus

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