Lipid composition of the toxic marine diatom, Nitzschia pungens

Parrish, Christopher C.; DeFreitas, A. S. W.; Bodennec, Guy; Macpherson, E. J.; Ackman, R. G.

doi:10.1016/0031-9422(91)84109-6

Cited by 42 publications

(21 citation statements)

References 18 publications

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“…Concentrations of FFA are highly species specific and can range from 0.4 to 26% of total lipids (Volkman et al 1989;Parrish et al 1991;Dunstan et al 1994). Berge et al (1995), however, asserts that high quantities of FFA in diatom cells are often artefacts, indicating lipolytic degradation and are, therefore, in unsuitable conditions for lipid extraction.…”

Section: Lipid Class Compositionmentioning

confidence: 99%

“…Additional FAs are more species-specific (Dunstan et al 1994). The FAs 16:1(n-7) and 20:5(n-3) are used as trophic markers for diatoms in algal blooms and associated food webs (Parrish et al 1991). The FA composition of N. cf.…”

Section: Fatty Acid Compostitionmentioning

confidence: 99%

“…perminuta. Such differences in the quantitative proportions can result from different growth conditions regarding nutrients, temperature and irradiance (Ackman et al 1968;Mortensen et al 1988;Parrish et al 1991). Decreasing temperature is one of the main factors which can shift the FA composition of membrane lipids from MUFA dominated to a more PUFA dominated composition (Mortensen et al 1988;Thompson et al 1992;Jiang and Gao 2004) to maintain the membrane fluidity (Murata and Los 1997).…”

Section: Fatty Acid Compostitionmentioning

confidence: 99%

“…Polar lipids are common membrane constituents consisting of high proportions of polyunsaturated fatty acids (PUFA), whereas TAG have generally more saturated (SFA) and monounsaturated fatty acids (MUFA) (Sukenik and Wahnon 1991). Concentrations of FFA in diatoms seem to be highly species specific and can range from 0.4 to 26% of total lipids (Volkman et al 1989;Parrish et al 1991;Dunstan et al 1994). The FA composition in diatoms has been studied intensively (Kates and Volcani 1966;Ackman et al 1968;Volkman et al 1989;Dunstan et al 1994), and reported as sensitive trophic and chemical marker for marine food webs (Kattner and Brockmann 1990;Parrish et al 1991;Graeve et al 1994) as well as an indicator for changes of environmental conditions (Kattner and Brockmann 1990).…”

Section: Introductionmentioning

confidence: 99%

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Effects of prolonged darkness and temperature on the lipid metabolism in the benthic diatom Navicula perminuta from the Arctic Adventfjorden, Svalbard

et al. 2017

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which could consequently lead to a depletion of this energy reserves before the end of the polar night. On the other hand, the membrane building phospho-and glycolipids remained unchanged during the 8 weeks darkness, indicating still intact thylakoid membranes. These results explain the shorter survival times of polar diatoms with increasing water temperatures during prolonged dark periods. Abstract The Arctic represents an extreme habitat for phototrophic algae due to long periods of darkness caused by the polar night (~4 months darkness). Benthic diatoms, which dominate microphytobenthic communities in shallow water regions, can survive this dark period, but the underlying physiological and biochemical mechanisms are not well understood. One of the potential mechanisms for long-term dark survival is the utilisation of stored energy products in combination with a reduced basic metabolism. In recent years, water temperatures in the Arctic increased due to an ongoing global warming. Higher temperatures could enhance the cellular energy requirements for the maintenance metabolism during darkness and, therefore, accelerate the consumption of lipid reserves. In this study, we investigated the macromolecular ratios and the lipid content and composition of Navicula cf. perminuta Grunow, an Arctic benthic diatom isolated from the microphytobenthos of Adventfjorden (Svalbard, Norway), over a dark period of 8 weeks at two different temperatures (0 and 7 °C). The results demonstrate that N. perminuta uses the stored lipid compound triacylglycerol (TAG) during prolonged dark periods, but also the pool of free fatty acids (FFA). Under the enhanced temperature of 7 °C, the lipid resources were used significantly faster than at 0 °C, Keywords

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Section: Lipid Class Compositionmentioning

confidence: 99%

Section: Fatty Acid Compostitionmentioning

confidence: 99%

Section: Fatty Acid Compostitionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of prolonged darkness and temperature on the lipid metabolism in the benthic diatom Navicula perminuta from the Arctic Adventfjorden, Svalbard

et al. 2017

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“…Notably, most biologically important fatty acids are synthesized de novo in phytoplanktons and are transferred to zooplanktons and other [22]. Small phytoplankton species including diatoms and flagellates contain significant amount of long chain PUFA (especially C20:5ω3 and C22:6ω3) as well as C16:0, C16:1ω7 and C14:0 [24] while larger phytoplanktons such as dinoflagellates, contain C20:5ω3, C22:6ω3, C16:0 and C18:4ω3 [25]. C16:1ω7 is believed to be a diatom marker, whereas C18:1ω9 is not restricted to a single phytoplankton group.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid composition of lipid classes in two mussel populations (Anodonta piscinalis and Corbicula fluminalis) living in Tigris River

Ekin¹,

Başhan²,

Şeşen³

2012

Turk J Bioch

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Purpose: The aim of this work was to investigate quantitative and qualitative fatty acid composition of phospholipid, monoacylglycerol, diacylglycerol, triacylglycerol and total body lipid of two adult freshwater mussels, Anodonta piscinalis (Nilsson, 1822) and Corbicula fluminalis (Müller, 1774). Methods: Mussels' body lipids were fractionated by thin layer chromatography. Gas chromatography and gas chromatography-mass spectrometry instruments were used to detect the fatty acids. Results: C16:0, C18:0, C16:1ω7, C18:1ω9, C18:2ω6 and C20:4ω6 were the dominant fatty acids. Their total percentages are between 70.37% and 82.11% in A. piscinalis and between 66.70% and 75.53% in C. fluminalis. As characteristic to freshwater mussels, Σω6 / Σω3 ratio was defined high in all lipid fractions. In both of the mussels, the maximum level of total polyunsaturated fatty acids was found in the phospholipids, and the maximum level of the total saturated fatty acids was found in the monoacylglycerol fractions. However, the level total monounsaturated fatty acids showed differentiations among fractions. In comparison to other fractions, the phospholipid contained high amount of C20:4ω6 and C20:5ω3, involved in eicosanoids formation. Conclusion:The study provided insight into the biochemistry and nutritional value of the widely distributed freshwater mussels from Tigris River. Key words: fatty acids, Anodonta piscinalis, Corbicula fluminalis, freshwater mussel, Tigris River Conflict of interest: Authors have no conflict of interest. ÖZETAmaç: Bu çalışmanın amacı, tatlısu midyesi Anodonta piscinalis (Nilsson, 1822) ve Corbicula fluminalis (Müller, 1774)'in fosfolipit, monoaçilgliserol, diaçilgliserol, triaçilgliserol ve total vücut lipidinin yağ asidi içeriğini kalitatif ve kantitatif olarak araştırmaktır. Metotlar: Midyelerin total vücut lipitleri, ince tabaka kromatografi ile fraksiyonlandı. Yağ asidi içeriği, gaz kromatografi ve gaz kromatografi-kütle spektrometre cihazları kullanılarak tespit edildi. Bulgular: C16:0, C18:0, C16:1ω7, C18:1ω9, C18:2ω6 ve C20:4ω6 asitler çoğunlukta bulunan bileşenlerdi. Toplam oranları A. piscinalis'te %70.37 ve %82.11 arasında; C. fluminalis'te ise %66.70 ve %75.53 arasında değişti. Tatlısu midyelerine özgü olduğu gibi, Σω6 / Σω3 değeri tüm fraksiyonlarda yüksek miktarda bulundu. Her iki midye türünde de en yüksek total çoklu doymamış yağ asidi oranı, fosfolipitte; en yüksek total doymuş yağ asidi oranı ise monoaçilgliserolda saptandı. Total tekli doymamış yağ asidi oranı ise fraksiyonlar arasında farklılık gösterdi. Diğer fraksiyonlar ile kıyaslanınca, fosfolipit fraksiyonunda eikosanoidlerin yapımında görev alan C20:4ω6 ve C20:5ω3 asitler daha yüksek oranda bulundu. Sonuç: Çalışma, Dicle Nehri'nde geniş dağılış gösteren tatlısu midyelerinin besinsel ve biyokimyasal özelliklerine ait bilgiler sağlamıştır. Anahtar kelimeler: yağ asitleri, Anodonta piscinalis, Corbicula fluminalis, tatlısu midyesi, Dicle Nehri Çıkar çatışması: Yazarların çıkar çatışması yoktur.

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Environmental stress and domoic acid production byPseudo-nitzschia: a physiological perspective

1998

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Lipid composition of the toxic marine diatom, Nitzschia pungens

Cited by 42 publications

References 18 publications

Effects of prolonged darkness and temperature on the lipid metabolism in the benthic diatom Navicula perminuta from the Arctic Adventfjorden, Svalbard

Effects of prolonged darkness and temperature on the lipid metabolism in the benthic diatom Navicula perminuta from the Arctic Adventfjorden, Svalbard

Fatty acid composition of lipid classes in two mussel populations (Anodonta piscinalis and Corbicula fluminalis) living in Tigris River

Environmental stress and domoic acid production byPseudo-nitzschia: a physiological perspective

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