Lipid content of marine microalgae Chaetoceros muelleri Lemmermann (Bacillariophyceae) grown at different salinities

Abstract

ResumoTeor lipídico da microalga marinha Chaetoceros muelleri Lemmermann (Bacillariophyceae) cultivada em diferentes salinidades. A produção e armazenamento de lipídios por microalgas em resposta a variações nos fatores ambientais são específicos para cada espécie de microalga. O presente trabalho teve como objetivo avaliar o crescimento e a concentração de lipídios totais da microalga Chaetoceros muelleri cultivada em três diferentes salinidades (15, 25 e 35). O experimento foi realizado em triplicatas, utilizando frascos de nove litros com volumes úteis de 7 L, sendo cultivo do tipo estacionário ou "batch". A temperatura da sala de cultivo e a intensidade luminosa foram mantidas em 28±1ºC e 20 μ.mol.m -2 .s -1 , respectivamente. Os resultados mostraram que no presente trabalho, o rendimento lipídico (10,41±1,89, 10,87±2,10, 12,33±1,81% nas salinidades 15, 25 e 35, respectivamente) não foi influenciado pela concentração salina (p>0,05). No entanto, a concentração celular máxima sofreu esta influência e foi significativamente maior (p<0,05) nas salinidades de 15 e 25 as quais obtiveram 16,3±1,50 e 17,6±2,90 x 10 5 cel.mL -1 , respectivamente. Key words: Cultivo; Lipídeos; Microalgas AbstractThe production and storage of lipids by microalgae in response to variations in environmental factors are specific for each microalgal species. The present study aimed to evaluate the growth and concentration of total lipids of the microalga C. muelleri Lemmermann cultivated at three different salinities (15, 25 and 35). The experiment was conducted in triplicate, using 9-L containers (7-L working volume), with the culture being stationary or batch type. The temperature and light intensity of the cultivation room was kept at 28±1ºC and 20 μ.mol.

Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors

“…ml -1 . This is similar with the research conducted by Barros et al which showed that the optimal growth of C.muelleri in his research occurs in salinity 25 ppt [2].…”

“…The lipid content in this phase is the highest compared to other phases. According to Barros et.al, the lipid content will increase dramatically in the stationary phase along with decreasing levels of nitrogen and silicates [2]. However, in continuous cultures, nutrient flow is always constant every day so that increased lipids during this phase are thought to be due to the cell's response to salinity.…”

“…The production of lipids produced by microalgae also 10 -20 times greater per unit area compared to agricultural products [1]. Marine microalgae species, especially diatoms, are very promising in producing high lipids [2]. One of them is Chaetoceros muelleri.…”

A comparative analysis of fatty acid composition and fucoxanthin content in six Phaeodactylum tricornutum strains from diff erent origins

“…Microalgal cell growth was monitored by measuring the OD 700 in a spectrophotometer (Rial et al, 2013;Shing et al, 2013;Barros et al, 2014). Microalgal cell dry weight concentration was calculated based on an optical density versus cell dry weight calibration curve.…”

Nutrient Absorption and Biomass Production by the Marine Diatom Chaetoceros muelleri: Effects of Temperature, Salinity, Photoperiod, and Light Intensity

“…Therefore, cultivating microalgae for obtaining not a single but multiple benefits simultaneously is receiving growing attention [ Chaetoceros muelleri is a marine diatom that is ubiquitously distributed worldwide. Because of its fast growth and easy maintenance, C. muelleri has been used in aquaculture as feed for zooplankton and shrimp larvae at commercial hatcheries in many regions [Wang et al 2014 Another reason that C. muelleri has been frequently used in aquaculture is its adaptation to salinity change [Fujii et al 1995;Barros et al 2014;Rahmadi et al 2020], which is likely to often occur in the land-based aquacultural ponds. Though the growth of Chaetoceros spp.…”