Lipid‐dependent coupling of secretory cargo sorting and trafficking at the trans‐Golgi network

Blume, Julia von; Haußer, Angelika

doi:10.1002/1873-3468.13552

Cited by 45 publications

(31 citation statements)

References 156 publications

(209 reference statements)

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“…Cab45 is a Ca 2+ -binding protein with six EF-hand domains that localizes in the lumen of the Golgi apparatus (Blank and von Blume, 2017;von Blume and Hausser, 2019;von Blume et al, 2012;Crevenna et al, 2016;Pakdel and von Blume, 2018). In the presence of Ca 2+ that has been locally pumped into the TGN by the Ca 2+ ATPase SPCA1, Cab45 oligomerizes and sorts cargos (von Blume et al, 2012;Crevenna et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Fam20C regulates protein secretion by Cab45 phosphorylation

Hecht

Blank

Steger

et al. 2020

Journal of Cell Biology

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The TGN is a key compartment for the sorting and secretion of newly synthesized proteins. At the TGN, soluble proteins are sorted based on the instructions carried in their oligosaccharide backbones or by a Ca2+-mediated process that involves the cargo-sorting protein Cab45. Here, we show that Cab45 is phosphorylated by the Golgi-specific protein kinase Fam20C. Mimicking of phosphorylation translocates Cab45 into TGN-derived vesicles, which goes along with an increased export of LyzC, a Cab45 client. Our findings demonstrate that Fam20C plays a key role in the export of Cab45 clients by fine-tuning Cab45 oligomerization and thus impacts Cab45 retention in the TGN.

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Section: Discussionmentioning

confidence: 99%

Fam20C regulates protein secretion by Cab45 phosphorylation

Hecht

Blank

Steger

et al. 2020

Journal of Cell Biology

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“…In summary, CARTS constitute a totally distinct class of carriers destined to the cell surface as they exclude tsVSV-G. However, several cargo proteins sorted by Cab45 (like lysozyme C) are associated with CARTS [145] raising the question: how similar are these carriers to SM secretory carriers discussed above? Further investigation is essential to fully understand what distinguishes these carriers from the other ones.…”

Section: Cartsmentioning

confidence: 97%

“…Lipids play a central role in the budding of carriers from the trans-Golgi (reviewed in [145]). One of the major lipids in this process is the phosphoinositide PI(4)P [105] see also section 5.1 above.…”

Section: Importance Of the Lipid Pi(4)p In Post-golgi Traffickingmentioning

confidence: 99%

Direct trafficking pathways from the Golgi apparatus to the plasma membrane

Stalder

Gershlick

2020

Seminars in Cell & Developmental Biology

106

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In eukaryotic cells, protein sorting is a highly regulated mechanism important for many physiological events. After synthesis in the endoplasmic reticulum and trafficking to the Golgi apparatus, proteins sort to many different cellular destinations including the endolysosomal system and the extracellular space. Secreted proteins need to be delivered directly to the cell surface. Sorting of secreted proteins from the Golgi apparatus has been a topic of interest for over thirty years, yet there is still no clear understanding of the machinery that forms the post-Golgi carriers. Most evidence points to these post-Golgi carriers being tubular pleomorphic structures that bud from the trans-face of the Golgi. In this review, we present the background studies and highlight the key components of this pathway, we then discuss the machinery implicated in the formation of these carriers, their translocation across the cytosol, and their fusion at the plasma membrane.Abbreviations: ATP, adenosine triphosphate; BFA, Brefeldin A; CARTS, CARriers of the TGN to the cell Surface; CI-MPR, cation-independent mannose-6 phosphate receptor; CtBP3/BARS, C-terminus binding protein 3/BFA adenosine diphosphate-ribosylated substrate; ER, endoplasmic reticulum; GlcCer, glucosylceramide; PAUF, pancreatic adenocarcinoma up-regulated factor; PM, plasma membrane; RUSH, retention using selective hooks; SBP, streptavidin-binding peptide; SM, sphingomyelin; SNARE, soluble N-ethylmaleimide sensitive fusion protein attachment protein receptor;SPCA1, secretory pathway calcium ATPase 1; TGN, trans-Golgi Network; TIRF, total internal reflection fluorescence; ts, temperature sensitive; VSV, vesicular stomatitis virus J o u r n a l P r e -p r o o f J o u r n a l P r e -p r o o f J o u r n a l P r e -p r o o f J o u r n a l P r e -p r o o f

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“…A central question in this field is how the transport stations communicate across each other and with other cell modules through signaling pathways. This question was addressed both at the meeting and in this special issue, through the perspective of signaling events occurring at the ER‐Golgi interface and at the Golgi .…”

Section: Autoregulation and Homeostasis Of The Transport Apparatusmentioning

confidence: 99%