Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy

Kahya, Nicoletta; Scherfeld, Dag; Bacia, Kirsten; Schwille, Petra

doi:10.1016/j.jsb.2003.09.021

Cited by 168 publications

(142 citation statements)

References 66 publications

Supporting

Mentioning

125

Contrasting

Order By: Relevance

“…The higher ZnO concentration leads to the enhancement of the D reduction. This is in line with other studies, which reported that a noticeable influence over lipid membranes is observed at higher concentrations of cholesterol, proteins or other nanoparticles [26][27][28][29][30][31][32]. This effect is explained by reduction of free area because of tighter lipid packing.…”

Section: Zno and Tio 2 Np's Interaction With Slbsupporting

confidence: 92%

Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

Grinceviciute¹,

Verdes²,

Snitka³

2015

JNMR

View full text Add to dashboard Cite

Nanoparticles (NP) are broadly exploited in biomedical sciences in order to develop various methods of targeted drug delivery, novel biosensors and new therapeutic pathways. However, relatively little is known in the negotiation of NPs with complex biological environments. NP interaction with cell membranes can damage the cell membrane and cause toxicity; therefore, examining interactions between NPs and cell membranes is crucial to understanding NP toxicity mechanisms and the development of safe and non-toxic NP-based commercial and therapeutic applications. To gain a physical understanding of NP-membrane interactions, we used a simplified system built of well-defined synthetic lipid bilayers and NP. The interaction of nanoparticles (NPs) with supported lipid bilayers (SLB) can lead to structural modification of the SLB and affect the structure and dynamic of lipids. In this work TiO 2 and ZnO nanoparticles were chosen because of wide applications and usage in industry of papers, inks, medicines, food products, cosmetics, toothpastes and skin care products and among others. Therefore, a better understanding of the interactions between NP and lipid membrane may help to better clarify the potential risk of NPs. The interaction between ZnO and TiO 2 NPs and lipid membranes was studied by using scanning supercritical angle fluorescence microscopy. The biological response to elucidated changes in lipid membrane structure/characteristics under ZnO or TiO 2 NP influence was tested by fluorescence correlation spectroscopy. It was found the significant reduction of lipids diffusion mobility, which can be explained as a result of lipid-ZnO aggregates binding, depending on ZnO concentration. The TiO 2 has a little effect on lipids diffusion mobility.

show abstract

Section: Zno and Tio 2 Np's Interaction With Slbsupporting

confidence: 92%

Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

Grinceviciute¹,

Verdes²,

Snitka³

2015

JNMR

View full text Add to dashboard Cite

show abstract

“…Furthermore, neither dye was enriched at the leading edge. A hallmark of liquid-ordered domains is their sensitive dependence on membrane cholesterol (Kahya et al, 2004). In a second approach, we therefore used methyl-␤-cyclo-dextran treatment to extract 35% of plasma membrane cholesterol from keratocytes prior to FLOID.…”

Section: The Barrier Does Not Depend On Lipid Microdomainsmentioning

confidence: 99%

The leading edge is a lipid diffusion barrier

Weisswange

Bretschneider

Anderson

2005

Journal of Cell Science

View full text Add to dashboard Cite

Actin polymerization drives many cellular events, including endocytosis, pathogen rocketing, and cell spreading. Force generation and polymerization regulation are intimately linked where an actin meshwork attaches to, and pushes against, an interface. We reasoned that interaction with actin filament plus-ends might stabilize the position of components within the plasma membrane at the leading edge, thereby slowing the diffusion of lipids within the bilayer where filament growth occurs. To test this hypothesis we focally labeled the outer membrane leaflet of migrating keratocytes and compared the initial diffusion of carbocyanine dyes in the dorsal and ventral lamellipodium membranes using sequential TIRF and epi-fluorescent imaging. Global diffusion analysis shows that lateral mobility of lipids in the outer membrane leaflet is blocked at the leading edge during protrusion. Cytochalasin treatment abolished this diffusion barrier, but we found no evidence to support the involvement of membrane microdomains. Our results demonstrate the immobilization of membrane components at the leading edge, and suggest that interaction between actin filaments and the plasma membrane is mediated by densely packed molecular complexes. We propose that actin polymerization traps regulatory proteins at the leading edge in a positive-feedback loop.

show abstract

“…[12][13][14][15][16][17][18] In this report, we measure the collective mobility of a number of membrane components as a function of temperature in living cell membranes by fluorescence correlation spectroscopy (FCS). [19][20] Since collective mobility exhibits a discontinuity as a system passes through a miscibility phase transition, [21][22] these observations are expected to reveal the phase transition, even in cases where the phase domains are too small to resolve by direct imaging. We support this by a variety of Monte-Carlo and atomistic simulations, which show that FCS measurements of tagged particle diffusion would capture such transitions.…”

Section: Introductionmentioning

confidence: 99%

Live Cell Plasma Membranes Do Not Exhibit a Miscibility Phase Transition over a Wide Range of Temperatures

et al. 2015

View full text Add to dashboard Cite

Lipid:cholesterol mixtures derived from cell membranes, as well as their synthetic reconstitutions, exhibit well defined miscibility phase transitions and critical phenomena near physiological temperatures. This suggests that lipid:cholesterol-mediated phase separation plays a role in the organization of live cell membranes. However, macroscopic lipid phase separation is not generally observed in cell membranes and the degree to which properties of isolated lipid mixtures are preserved in the cell membrane remain unknown. A fundamental property of phase transitions is that the variation of tagged particle diffusion with temperature exhibits an abrupt change as the system passes through the transition, even when the two phases are distributed in a nanometer-scale emulsion. We support this using a variety of Monte-Carlo and atomistic simulations on model lipid membrane systems. However, temperature dependent fluorescence correlation spectroscopy of labeled lipids and membrane-anchored proteins in live cell membranes show a consistently smooth increase of the diffusion coefficient as a function of temperature. We find no evidence of a discrete miscibility phase transition throughout a wide range of temperatures: 14 -37 °C. This contrasts the behavior of giant plasma membrane vesicles (GPMVs) blebbed from the same cells, which do exhibit phase transitions and macroscopic phase separation. Fluorescence lifetime analysis of a DiI probe in both cases reveals a significant environmental difference between the live cell and the GPMV. Taken together, these data suggest the live cell membrane may avoid the miscibility phase transition inherent to its lipid constituents by actively regulating physical paramters, such as tension, in the membrane.

show abstract

Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy

Cited by 168 publications

References 66 publications

Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

The leading edge is a lipid diffusion barrier

Live Cell Plasma Membranes Do Not Exhibit a Miscibility Phase Transition over a Wide Range of Temperatures

Contact Info

Product

Resources

About