BACKGROUNDThe leaves of guabirobeira contain important phenolic and flavonoid compounds, known for their antioxidant, anti‐inflammatory, antibacterial, and antinociceptive activities. The purpose of this work was to obtain extracts from guabirobeira leaves through aqueous infusion (AI) and pressurized liquid extraction with water (PLE‐W), ethanol (PLE‐E), and a combination of water:ethanol ratio 1:1, v/v (PLE‐W:E). Subsequently, to identify the chemical compounds using liquid chromatography coupled with mass spectrometry, and to study the antioxidant activity in rat brain cells, thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS) antibacterial activity against Gram‐negative and Gram‐ positive bacteria is also seen.RESULTSAccording to the findings, all four extracts contained a significant amount of phytochemical compounds. Among them, the PLE‐W:E extract showed a higher concentration of flavonoids, demonstrating significant antioxidant activity in in vitro assays of TBARS and ROS in rat brain cells. It also exhibited antibacterial activity against both bacteria (Gram‐positive and Gram‐negative).CONCLUSIONAs a result, it can be concluded that guabirobeira leaf extracts hold promise as a potential source of bioactive compounds. These extracts can be applied across various sectors, including cosmetics and skincare products, pharmaceuticals, food and beverages, natural preservatives, and functional materials. By exploring these potential applications, valuable contributions can be made to the field of sustainable chemistry and pharmacy, furthering the utilization of guabirobeira leaf extracts in diverse industries. This approach also aligns with the promotion of environmentally friendly and sustainable practices.This article is protected by copyright. All rights reserved.