Lipid lateral diffusion by pulsed nuclear magnetic resonance

Kuo, An‐Li; Wade, Charles G.

doi:10.1021/bi00578a026

Cited by 192 publications

(109 citation statements)

References 57 publications

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“…Further, a variety of experimental methods report significantly different values for the diffusion coefficient. Experimental studies using pulsed field gradient nuclear magnetic resonance, 46 single particle tracking, 47 fluorescence recovery after photobleaching, 48 or from fluorescence correlation spectroscopy 49 report values within 0.5 × 10 −7 cm 2 /s and 1 × 10 −7 cm 2 /s. Neutron scattering experiments, operating in the picosecond range, produce diffusion coefficients significantly higher, 7,8,50 in the range of 1 to 10 × 10 −7 cm 2 /s.…”

Section: Mechanisms Of Lipid Diffusionmentioning

confidence: 99%

Diffusion and spectroscopy of water and lipids in fully hydrated dimyristoylphosphatidylcholine bilayer membranes

Yang

Calero

Martı́

2014

The Journal of Chemical Physics

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Microscopic structure and dynamics of water and lipids in a fully hydrated dimyristoylphosphatidylcholine phospholipid lipid bilayer membrane in the liquid-crystalline phase have been analyzed with all-atom molecular dynamics simulations based on the recently parameterized CHARMM36 force field. The diffusive dynamics of the membrane lipids and of its hydration water, their reorientational motions as well as their corresponding spectral densities, related to the absorption of radiation, have been considered for the first time using the present force field. In addition, structural properties such as density and pressure profiles, a deuterium-order parameter, surface tension, and the extent of water penetration in the membrane have been analyzed. Molecular self-diffusion, reorientational motions, and spectral densities of atomic species reveal a variety of time scales playing a role in membrane dynamics. The mechanisms of lipid motion strongly depend on the time scale considered, from fast ballistic translation at the scale of picoseconds (effective diffusion coefficients of the order of 10 −5 cm 2 /s) to diffusive flow of a few lipids forming nanodomains at the scale of hundreds of nanoseconds (diffusion coefficients of the order of 10 −8 cm 2 /s). In the intermediate regime of subdiffusion, collisions with nearest neighbors prevent the lipids to achieve full diffusion. Lipid reorientations along selected directions agree well with reported nuclear magnetic resonance data and indicate two different time scales, one about 1 ns and a second one in the range of 2-8 ns. We associated the two time scales of reorientational motions with angular distributions of selected vectors. Calculated spectral densities corresponding to lipid and water reveal an overall good qualitative agreement with Fourier transform infrared spectroscopy experiments. Our simulations indicate a blue-shift of the low frequency spectral bands of hydration water as a result of its interaction with lipids. We have thoroughly analyzed the physical meaning of all spectral features from lipid atomic sites and correlated them with experimental data. Our findings include a "wagging of the tails" frequency around 30 cm −1 , which essentially corresponds to motions of the tail-group along the instantaneous plane formed by the two lipid tails, i.e., in-plane oscillations are clearly of bigger importance than those along the normal-to-the plane direction. © 2014 AIP Publishing LLC.

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Section: Mechanisms Of Lipid Diffusionmentioning

confidence: 99%

Diffusion and spectroscopy of water and lipids in fully hydrated dimyristoylphosphatidylcholine bilayer membranes

Yang

Calero

Martı́

2014

The Journal of Chemical Physics

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“…Translational molecular mobility in model membranes has been studied by various physical methods including electron spin resonance (ESR), fluorescence recovery after photobleaching (FRAP) and others [1][2][3][4]. Pulsed field gradient nuclear magnetic resonance (PFG NMR) spectroscopy has been used extensively for diffusion measurements for many years [5][6][7][8][9] and is one of the most attractive methods for investigations of molecular transport in lipid membranes as no perturbing labeling of the molecules under study is necessary.…”

Section: Introductionmentioning

confidence: 99%

“…Translational diffusion of water and lipids in lamellar liquid crystalline phases has been studied by PFG NMR in a number of investigations [5][6][7][8][9][10][11][12]. For those studies NMR technique of the sample orientation at the magic angle has been applied, because for molecules with large dipole couplings, such as amphiphilic molecules in lamellar liquid crystalline phases, the NMR method removing the dipole interaction becomes necessary.…”

Section: Introductionmentioning

confidence: 99%

Water diffusivity in model biological membranes

2004

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Water self-diffusion was studied in model biological membranes (lipid bilayers of dioleoylphosphatidylcholine (DOPC) and dimyristoylphosphatidylcholine) by nuclear magnetie resonance with pulsed field gradient. The results for DOPC-water bilayers for three different orientations of the angle 0 between the direction of the field gradient and the normal to the bilayer (0 = 57.4, 90, 0 o) were presented. The differences in the diffusion deeay shapes and values of the diffusion coefficients, obtained at different bilayer o¡ and demonstrating highly anisotropic diffusion of the interbilayer water was discussed. This study also has shown some features of water diffusion in model lipid bilayers at the concentrations corresponding to the presence of bound and quasi-free water. The dependence of the water and lipid diffusion on the water content was considered from the point of view of the bilayers hydration and types of interbilayer water.

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“…For example, Thompson and Axelrod reported that a 30% cholesterol depletion of human erythrocyte membrane was not accompanied by a significant reduction of lipid lateral diffusion at 37 °C (Thompson and Axelrod 1980). In lipid bilayers comprising a single phospholipid species and cholesterol, no investigation has demonstrated that cholesterol enrichment is sufficient to account for, at a quantitative level, the difference in physical properties observed in RBC between the two leaflets; a factor of about 2 is the maximum effect reported (Devaux and McConnell 1972;Wu et al 1977;Rubenstein et al 1979;Kuo and Wade 1979;Lindbloom et al 1981;Shin and Freed 1989).…”

Section: Introductionmentioning

confidence: 99%

Lateral diffusion of erythrocyte phospholipids in model membranes comparison between inner and outer leaflet components

Cribier

Morrot

et al. 1990

Eur Biophys J

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The physical properties of lipid bilayers with a similar composition to the outer and inner leaflets of the human erythrocyte membrane have been examined in protein-free model systems. The outer leaflet (OL) was represented by a phospholipid mixture containing phosphatidylcholine and sphingomyelin extracted from human erythrocytes, while a mixture of phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine represented the inner leaflet (IL). The ratio of cholesterol to phospholipid was varied in both mixtures. The lateral diffusion coefficient of fluorescent phospholipids diluted in such lipid mixtures was determined by the modulated fringe pattern photobleaching technique. Contrast curves with a single exponential decay, indicative of homogeneous samples, were obtained only for temperatures above 15 degrees C and for a cholesterol to phospholipid molar ratio below 0.8. The rate of lateral diffusion was approximately five times faster in IL than in OL multilayers, in agreement with former results obtained in human erythrocytes (Morrot et al. 1986). Varying the cholesterol to phospholipid ratio from 0 to 0.8 (mol/mol) enabled us to decrease the diffusion constant by only a factor of approximately 2 for both IL and OL mixtures. The order parameter of a spin-labeled phospholipid was determined in the different systems and found to be systematically smaller in IL mixtures than in OL mixtures. The present study indicates that the difference in lipid diffusivity of the two erythrocyte leaflets may be accounted for solely by a difference in phospholipid composition, and may be independent of cholesterol and protein asymmetry.

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Lipid lateral diffusion by pulsed nuclear magnetic resonance

Cited by 192 publications

References 57 publications

Diffusion and spectroscopy of water and lipids in fully hydrated dimyristoylphosphatidylcholine bilayer membranes

Diffusion and spectroscopy of water and lipids in fully hydrated dimyristoylphosphatidylcholine bilayer membranes

Water diffusivity in model biological membranes

Lateral diffusion of erythrocyte phospholipids in model membranes comparison between inner and outer leaflet components

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