2008
DOI: 10.1194/jlr.m700421-jlr200
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Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging

Abstract: Human striated muscle samples, from male control and Duchenne muscular dystrophy-affected children, were subjected to cluster-time-of-flight secondary ion mass spectrometry (cluster-ToF-SIMS) imaging using a 25 keV Bi 3 1 liquid metal ion gun under static SIMS conditions. Spectra and ion density maps, or secondary ion images, were acquired in both positive and negative ion mode over several areas of 500 3 500 mm 2 (image resolution, 256 3 256 pixels). Characteristic distributions of various lipids were observe… Show more

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Cited by 88 publications
(81 citation statements)
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“…The LPA plaque apoptosis was not altered upon LPA challenge, despite the preferential secretion of tryptase, previously shown by us and others ( 6,33 ) as main culprit in mast cellassociated apoptosis. Possibly the pro-apoptotic effects of the mast cell secretome are counteracted by the intrinsic histone deacetylase and serine threonine kinase (Akt)-dependent anti-apoptotic effects of LPA itself ( 27,34 ). However, in our in vitro studies LPA was not able to rescue macrophages from tryptase induced apoptosis, rendering this theory unlikely.…”
Section: Local Lpa Treatment and Plaque Morphologycontrasting
confidence: 51%
See 1 more Smart Citation
“…The LPA plaque apoptosis was not altered upon LPA challenge, despite the preferential secretion of tryptase, previously shown by us and others ( 6,33 ) as main culprit in mast cellassociated apoptosis. Possibly the pro-apoptotic effects of the mast cell secretome are counteracted by the intrinsic histone deacetylase and serine threonine kinase (Akt)-dependent anti-apoptotic effects of LPA itself ( 27,34 ). However, in our in vitro studies LPA was not able to rescue macrophages from tryptase induced apoptosis, rendering this theory unlikely.…”
Section: Local Lpa Treatment and Plaque Morphologycontrasting
confidence: 51%
“…The LPA assigned peaks detected in SIMS imaging analysis of plaque lysates are identical to m/z values obtained by LC/MS spectroscopy, thus providing an orthogonal validation of the SIMS results. Further analysis of the identifi ed LPA peaks using the database provided by the group of Brunelle ( 27 ) shows a lack of overlap of the most typical fragments with DAG-H 2 O and free fatty acid peaks, making it unlikely that the former result from phospholipid fragmentation. …”
Section: Mast Cell Releasatementioning
confidence: 99%
“…This method now enables drawing from various kinds of samples the density map (image) of any ion present in the mass spectra, with a spatial resolution from a few m to less than 500 nm, and with a mass range which is now reaching more than 1000 Da. In recent years, many applications of TOF-SIMS imaging have been published in the fields of biology and medicine [32][33][34][35][36][37][38][39][40][41][42][43]. However, it has never been directly applied to the exploration of persistent organic pollutants such as environmental or food contaminants.…”
mentioning
confidence: 99%
“…This technology consists of the bombardment of the sample by a beam of mono-or polyatomic ions, which induces desorption/ ionization of secondary ions from the sample surface. Polyatomic ion beams have been used successfully in the analysis of organic surfaces with a resolution of some hundreds of nanometers (19)(20)(21)(22)(23)(24), which makes the technology particularly fi t for the analysis of tissue sections ( 19,22,(25)(26)(27)(28)(29)(30)(31)(32). Conversely, MALDI uses a matrix to allow soft desorption/ionization of intact compounds upon laser absorption; it is also adapted to imaging analysis but at a spatial resolution limited to ‫ف‬ 50 µm with the current stateof-the-art.…”
Section: Multivariate Analysismentioning
confidence: 99%