Lipid Markers of Breast Tissue for the Diagnosis of Regional Metastatic Lesion

Abstract

The development of minimally invasive, non-traumatic and stable approaches for the diagnosis of metastatic lesions of regional lymph nodes upon breast cancer is of great urgency. Here we recorded lipid profiles of normal breast tissue and malignant tissue to reveal potential lipid markers of metastatic lesions of regional lymph nodes. Lipid identification was done using the Lipid Match package. The search for lipid markers was carried out using the Mann-Whitney test. Lipids for the construction of a diagnostic logistic regression were selected according to the Akaike information criterion. For normal breast tissue, a diagnostic model was obtained with the area under the curve (AUC) of 0.83; for tumor tissue, a model with AUC = 0.86 was obtained. The species PC 14:0_20:4, PE 18:1_20:1, PC P-16:0/20:4, PC P-16:0/20:4, PE P-16:0/22:4, SM d18:1/18 0, SM d18:1/22:0 were determined as markers for normal breast tissue. The species PC 18:2_22:6, PC O-18:0/20:2, SM d16:1/18:1, SM d22:0/20:2, SM d16:0/18:2 were determined as markers for tumor tissue. The high AUC values ​​for the developed diagnostic model indicate the potential significance of the revealed marker species for the diagnosis of breast cancer metastasis and indicate the need for further research in this direction.