2021
DOI: 10.1590/0001-3765202120200019
|View full text |Cite
|
Sign up to set email alerts
|

Lipid-matrix effects on tyrosinase immobilization in Langmuir and Langmuir-Blodgett films

Abstract: The immobilization of the enzyme tyrosinase (Tyr) in lipid matrices can be explored to produce biosensors for detecting polyphenols, which is relevant for the food industry. Herein, we shall demonstrate the importance of the lipid composition to immobilize the enzyme tyrosinase in Langmuir-Blodgett (LB) fi lms. Tyr could be incorporated into Langmuir monolayers of arachidic acid (AA), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (DP… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
3

Relationship

1
2

Authors

Journals

citations
Cited by 3 publications
(2 citation statements)
references
References 51 publications
0
2
0
Order By: Relevance
“…389 The enzyme tyrosinase (Tyr) was incorporated into LB films of different lipids to detect polyphenols relevant to the food industry. 390 In the latter example, PM-IRRAS was used not only to investigate enzyme adsorption in Langmuir monolayers but also to determine the molecular-level interactions responsible for the detection. Enhanced sensitivity in lipidbased LB biosensors was achieved by introducing carbon nanotubes (CNT) in stearic acid films that contained asparaginase (ASNase).…”
Section: Biosensorsmentioning
confidence: 99%
See 1 more Smart Citation
“…389 The enzyme tyrosinase (Tyr) was incorporated into LB films of different lipids to detect polyphenols relevant to the food industry. 390 In the latter example, PM-IRRAS was used not only to investigate enzyme adsorption in Langmuir monolayers but also to determine the molecular-level interactions responsible for the detection. Enhanced sensitivity in lipidbased LB biosensors was achieved by introducing carbon nanotubes (CNT) in stearic acid films that contained asparaginase (ASNase).…”
Section: Biosensorsmentioning
confidence: 99%
“…For instance, the enzyme polyphenol oxidase (PPO) was immobilized in LB films with a positively charged lipid, octadecylamine (ODA+), to detect pyrocatechol . The enzyme tyrosinase (Tyr) was incorporated into LB films of different lipids to detect polyphenols relevant to the food industry . In the latter example, PM-IRRAS was used not only to investigate enzyme adsorption in Langmuir monolayers but also to determine the molecular-level interactions responsible for the detection.…”
Section: Lb Films Used In Molecular (Organic) Electronics and Sensorsmentioning
confidence: 99%