Lipid metabolic disorders and physiological stress caused by a high-fat diet have lipid source-dependent effects in juvenile black seabream Acanthopagrus schlegelii

Abstract

This study was conducted to evaluate the effects of different dietary lipid sources on growth performance, lipid metabolism and physiological stress responses including oxidative stress (OS) and endoplasmic reticulum stress (ERS) of juvenile Acanthopagrus schlegelii (initial weight 0.88 ± 0.01 g) fed a high fat diet (HFD). Four isonitrogenous and isolipidic experimental diets containing different lipid sources were formulated: sh oil (FO), palm oil (PO), linseed oil (LO) and soybean oil (SO), respectively. Results indicated that sh fed HFD supplemented with FO signi cantly improved growth than SO treatment. The high concentrations of aspartate aminotransferase and alanine transaminase were found in HFD supplemented with SO. Fish fed dietary LO supplementation showed signi cantly lower serum cholesterol, triglyceride, low-density lipoprotein and high-density lipoprotein contents than those in SO group. Likewise, hepatic para n section analysis indicated that HFD with PO or SO supplementation increased fat drop. The expression levels of peroxisome proliferators-activated receptor alpha (pparα) and silent regulator 1 (sirt1) were signi cantly elevated by HFD with FO or LO supplementation. Additionally, the key marker of OS malonaldehyde was signi cantly increased in FO and SO groups. ERS related genes were activated in dietary PO or SO supplementation and, hence, triggering in ammation and apoptosis by promoted the expression levels of nuclear factor kappa B (nf-κb) and c-Jun N-terminal kinase (jnk). Overall, the present study reveals that lipid metabolic disorders and physiological stress caused by a HFD have signi cant lipid source dependent effects, which have important guiding signi cance for the use of HFD in marine sh. Calculations and statistical analysis