Lipid metabolism in the northern pike (Esox LuciusL.)—3. In vivo incorporation of 1-14C-acetate in the lipids

Kluytmans, J.H.; Zandee, D.I.

doi:10.1016/0305-0491(74)90144-8

Cited by 9 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems likely that the majority of dietary fatty acids were either catabolised or, in the case PUFA, were selectively incorporated into the phospholipid pool, since this lipid class showed a very stable fatty acid composition throughout the study (see Table 4). This can be corroborated by the observed retention of I4C labelled PUFA into the polar lipids of fasting adult pike as reported by Kluytmans and Zandee (1974). Similarly, Castledine and Buckley (1982).…”

Section: Discussionsupporting

confidence: 86%

The effect of invertebrate diets on lipids, fatty acid composition and physiological condition of pike larvae (Esox lucius)

1997

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 86%

The effect of invertebrate diets on lipids, fatty acid composition and physiological condition of pike larvae (Esox lucius)

1997

View full text Add to dashboard Cite

“…The assumption that muscle PCB concentration was equal to the PCB concentration of all of the somatic tissue in the female northern pike was likely invalid, based on results from previous studies. Northern pike is a relatively lean fish [ 31 ], as is yellow perch ( Perca flavescens ) [ 32 ]. In a mixed-sex sample of yellow perch, whole-fish PCB concentration was 10 times higher than skin-off fillet PCB concentration [ 33 ].…”

Section: Sex Difference In Pcb Concentrationsmentioning

confidence: 99%

Sex differences in contaminant concentrations of fish: a synthesis

et al. 2016

View full text Add to dashboard Cite

A comparison of whole-fish polychlorinated biphenyl (PCB) and total mercury (Hg) concentrations in mature males with those in mature females may provide insights into sex differences in behavior, metabolism, and other physiological processes. In eight species of fish, we observed that males exceeded females in whole-fish PCB concentration by 17 to 43 %. Based on results from hypothesis testing, we concluded that these sex differences were most likely primarily driven by a higher rate of energy expenditure, stemming from higher resting metabolic rate (or standard metabolic rate (SMR)) and higher swimming activity, in males compared with females. A higher rate of energy expenditure led to a higher rate of food consumption, which, in turn, resulted in a higher rate of PCB accumulation. For two fish species, the growth dilution effect also made a substantial contribution to the sex difference in PCB concentrations, although the higher energy expenditure rate for males was still the primary driver. Hg concentration data were available for five of the eight species. For four of these five species, the ratio of PCB concentration in males to PCB concentration in females was substantially greater than the ratio of Hg concentration in males to Hg concentration in females. In sea lamprey (Petromyzon marinus), a very primitive fish, the two ratios were nearly identical. The most plausible explanation for this pattern was that certain androgens, such as testosterone and 11-ketotestosterone, enhanced Hg-elimination rate in males. In contrast, long-term elimination of PCBs is negligible for both sexes. According to this explanation, males not only ingest Hg at a higher rate than females but also eliminate Hg at a higher rate than females, in fish species other than sea lamprey. Male sea lamprey do not possess either of the above-specified androgens. These apparent sex differences in SMRs, activities, and Hg-elimination rates in teleost fishes may also apply, to some degree, to higher vertebrates including humans. Our synthesis findings will be useful in (1) developing sex-specific bioenergetics models for fish, (2) developing sex-specific risk assessment models for exposure of humans and wildlife to contaminants, and (3) refining Hg mass balance models for fish and higher vertebrates.

show abstract

“…The long-chain PUFA (LC-PUFA), arachidonic acid (20:4n-6, ARA), eicosapentaenoic acid (20:5n-3, EPA), and docosahexaenoic acid (22:6n-3, DHA), which have essential functions in vertebrate immune defense systems and neuronal membranes [14-16] can be produced endogenously, in some but not all vertebrates, from the base EFA, α-linolenic acid (18:3n-3, ALA) and linoleic acid (18:2n-6, LA), by desaturation and elongation [17]. The capability to produce LC-PUFA from EFA varies between fish species [13,18], and salmonids, including Atlantic salmon, brown trout ( Salmo trutta ), and Arctic charr ( Salvelinus alpinus ) have substantially higher LC-PUFA biosynthetic efficiency in comparison with other freshwater species, including zebrafish ( Danio rerio ), Nile tilapia ( Oreochromis niloticus ) [11,19,20], and Northern Pike [21-24]. The ability to produce LC-PUFA is dependent on the possession and expression of fatty acyl desaturase ( fad ) and fatty acid elongase ( elovl ) genes, and salmonids, in contrast to many other fish species examined, have a complete set of genes and expressed enzymes required for the production of ARA from LA, and EPA and DHA from ALA [25,26].…”

Section: Introductionmentioning

confidence: 99%

An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon

et al. 2013

View full text Add to dashboard Cite

BackgroundThe ability to produce physiologically critical LC-PUFA from dietary fatty acids differs greatly among teleost species, and is dependent on the possession and expression of fatty acyl desaturase and elongase genes. Atlantic salmon, as a result of a recently duplicated genome, have more of these enzymes than other fish. Recent phylogenetic studies show that Northern pike represents the closest extant relative of the preduplicated ancestral salmonid. Here we characterise a pike fatty acyl elongase, elovl5, and compare it to Atlantic salmon elovl5a and elovl5b duplicates.ResultsPhylogenetic analyses show that Atlantic salmon paralogs are evolving symmetrically, and they have been retained in the genome by purifying selection. Heterologous expression in yeast showed that Northern pike Elovl5 activity is indistinguishable from that of the salmon paralogs, efficiently elongating C18 and C20 substrates. However, in contrast to salmon, pike elovl5 was predominantly expressed in brain with negligible expression in liver and intestine.ConclusionsWe suggest that the predominant expression of Elovl5b in salmon liver and Elovl5a in salmon intestine is an adaptation, enabled by genome duplication, to a diet rich in terrestrial invertebrates which are relatively poor in LC-PUFA. Pike have retained an ancestral expression profile which supports the maintenance of PUFA in the brain but, due to a highly piscivorous LC-PUFA-rich diet, is not required in liver and intestine. Thus, the characterisation of elovl5 in Northern pike provides insights into the evolutionary divergence of duplicated genes, and the ecological adaptations of salmonids which have enabled colonisation of nutrient poor freshwaters.

show abstract

Lipid metabolism in the northern pike (Esox LuciusL.)—3. In vivo incorporation of 1-14C-acetate in the lipids

Cited by 9 publications

References 12 publications

The effect of invertebrate diets on lipids, fatty acid composition and physiological condition of pike larvae (Esox lucius)

The effect of invertebrate diets on lipids, fatty acid composition and physiological condition of pike larvae (Esox lucius)

Sex differences in contaminant concentrations of fish: a synthesis

An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon

Contact Info

Product

Resources

About