Lipid Metabolism in the Rats With Fatty Liver Caused by Low Protein Diet and Effects of the Oral Administration of L-Methionine, L-Cysteine, Pantethine and Calcium Pantothenate Upon It

Abstract

It has been generally known that feeding rats the low protein diet results in an accu mulation of lipids in the liver. Fatty livers of rats caused by the imbalance of amino acids in the body were reported to be produced by excessive or deficient sulfur-containing amino acids (1, 2) as well as by deficient lysine and threonine in the diet (3). It was reported by Ashida that the extent of lipid accumulation in the liver of rats was more marked with albumin than with casein as a dietary source of protein, and that the increase of sulfur-con taining amino acids content in the low protein diet activates the development of fatty liver (2). Moreover, he showed that the variation in the mixing ratio of methionine and cystine in the artificial low protein diet did not significantly affect the extent of the hepatic neutral fat.Methionine and cysteine are precursor amino acids for the physiological synthesis of coenzyme A in the mammalian species.Since coenzyme A correlates essentially the metabolic process of carbohydrates, pro tein, fat and steroid substances as well as the detoxication mechanism including the acetyl ation, effects of the agents relating to the endogenous formation of coenzyme A on the hepatic lipids accumulation were studied in this experiment.In the present experiments a fatty liver was produced by feeding rats the low protein diet in an attempts to know the possible development of fatty livers caused by the imba lance of amino acids, to compare the prophylactic and therapeutic effects of pantethine, calcium pantothenate, methionine and cysteine on the fatty liver and to establish some cor relation, if possible, between the activity of coenzyme A and the level of lipid in the liver. METHODSThe experimental animals used were young male rats of Wistar strain weighing about 110 g at the start of experiments. The animals kept in the individual cage at the room temperature of 22± l °C were maintained on the commercial standard diet (CLEA, CA-1) and drinking water ad libitum. The low protein diet used in the present experiments con

Efeito da dieta hiperlipídica e do treinamento aeróbico na aterosclerose em camundongos apoE-/-

“…O aumento na deposição de gordura no fígado pode ser também ocasionado devido ao aumento na disponibilidade de AGLs, com concomitante ausência de aumento na β-oxidação 31 . Outro fato que pode ser levado em consideração é a possibilidade de um controle endócrino gerado pelos animais alimentados com dieta rica em lipídeos, gerando maior produção hepática de gorduras e menor utilização destas com consequente acúmulo hepático 32 . Nesse sentido, sugere-se que o aumento do PRF encontrado em nosso estudo pode ser devido a uma maior quantidade de gordura depositada nele, pois em estudos que analisaram o efeito da dieta hiperlipídica, foi verificado um maior acúmulo de gordura no fígado com consequente presença de esteatose hepática 33 .…”