Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects

Chang, Lulu; Lu, Hengqian; Chen, Haiqin; Tang, Xin; Zhao, Jianxin; Zhang, Hao; Chen, Yong Q.

doi:10.1016/j.biotechadv.2021.107794

Cited by 46 publications

(48 citation statements)

References 209 publications

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“…[55], Mortierella sp. [56,57], and the Mucor sp., especially Mucor circinelloides [58,59]. A recent study revealed the fungus Cunninghamella echinulata increases the yield of GLA to 1.31 g L −1 and 1.28 g L −1 when 1% soybean powder or 0.5% potassium nitrate are added to the culture medium, respectively [60].…”

Section: Oleaginous Fungimentioning

confidence: 99%

Prospect of metabolic engineering in enhanced microbial lipid production: review

Saha

Mukhopadhyay²

2021

Biomass Conv. Bioref.

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The use of fossil fuels has increasingly become associated with negative influences like detrimental environmental effects. This has made renewables, especially biofuels like biodiesel, a highly attractive substitute for energy generation. The industrial and economic potential of biodiesel-a lipid-based fuel-has increased interest and research into oleaginous microorganisms to synthesise and produce lipids. Effective identification and characterisation of a vast array of oleaginous microorganisms have led them to be employed for their efficient lipogenesis capability and ability to use a wide range of synthetic and non-expensive substrates. However, low lipid production has limited the use of microbially sourced lipids for biodiesel production at the industrial level. Nevertheless, the improvement and engineering of robust strains of these microbes through metabolic engineering have increased their lipid production capacity, leading to commercialising the lipids. This review provides a comprehensive outlook into the identification of different oleaginous microorganisms and their unique characteristics, which makes them highly valuable. An insight into the lipid biosynthesis pathways is provided and the role that several enzymes and regulators play in the metabolism of lipid accumulation. A detailed outlook is provided on the broad range of metabolic engineering approaches with regard to enhanced lipid production in several oleaginous microorganisms.

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Section: Oleaginous Fungimentioning

confidence: 99%

Prospect of metabolic engineering in enhanced microbial lipid production: review

Saha

Mukhopadhyay²

2021

Biomass Conv. Bioref.

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“…At the same time, the products from these degradation processes provide the carbon skeleton and energy required for fatty acid synthesis ( 6 ). Mortierella alpina strains are oleaginous model strains that can accumulate more than 50% lipids under nitrogen-limiting conditions ( 17 , 18 ). Our previous multiomics analysis indicated that cell autophagy was significantly upregulated in M. alpina during the nitrogen stress-induced TAG accumulation process ( 19 ).…”

Section: Introductionmentioning

confidence: 99%

Autophagy Improves ARA-Rich TAG Accumulation in Mortierella alpina by Regulating Resource Allocation

Chen

Tang

et al. 2022

Microbiol Spectr

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“…Biomanufacturing based on oleaginous microorganisms has attracted attention in the health and nutrition industry to meet the ever-increasing demand for long-chain polyunsaturated fatty acids (LC-PUFAs). The arachidonic acid (ARA, C20: 4) producer Mortierella alpina and the docosahexaenoic acid (DHA, C22: 6) producer Aurantiochytrium limacinum are the most widely studied and applied microorganisms, and they have become the main sources for dietary LC-PUFAs certificated by the U.S. Food and Drug Administration (FDA) …”

Section: Introductionmentioning

confidence: 99%

“…For heterotrophic oleaginous microorganisms, the glucose utilization rate is a determinant factor that affects fermentation cost and final lipid yield . To enhance glucose utilization, genes involved in glucose transportation and metabolism have been modified to strengthen the allocation of carbon flux toward lipid synthesis. , In addition, some protein kinases with global regulatory roles also attract research interest. , Among them, the heterotrimeric sucrose nonfermentable protein kinase 1 (SNF1), homolog of mammal adenosine monophosphate-activated protein kinase (AMPK), is one of the well-known global regulators that plays a vital role in lipogenesis and cellular responses to nutrient levels. The first known conserved function of the SNF1 complex is the phosphorylation inhibition of acetyl-CoA carboxylase 1 (ACC1), the first committed step in fatty acid synthesis. , In Y.…”

Section: Introductionmentioning

confidence: 99%

“…The arachidonic acid (ARA, C20: 4) producer Mortierella alpina and the docosahexaenoic acid (DHA, C22: 6) producer Aurantiochytrium limacinum are the most widely studied and applied microorganisms, and they have become the main sources for dietary LC-PUFAs certificated by the U.S. Food and Drug Administration (FDA). 6 Currently, research goals in developing the biotechnological application of oleaginous microorganisms focus on enhancing target PUFA yield and reducing the production cost. 7 For heterotrophic oleaginous microorganisms, the glucose utilization rate is a determinant factor that affects fermentation cost and final lipid yield.…”

Section: ■ Introductionmentioning

confidence: 99%

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SNF1β-Modulated Glucose Uptake and the Balance between Polyunsaturated Fatty Acids and Carbohydrates in Mortierella alpina

Chang

Tang

Zhang

et al. 2021

J. Agric. Food Chem.

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A sucrose nonfermenting protein kinase 1 (SNF1) complex is an important metabolic regulator in fungi that is critical to cell metabolism and stress response. In this study, the role of an SNF1 β-subunit in the oleaginous fungus Mortierella alpina (MaSip2) was investigated. The MaSip2 contained a glycogen-binding domain and a conserved SNF1-complex interaction region; its transcriptional level during lipogenesis shared high consistency with a previously reported SNF1 γ-subunit (MaSnf4). Overexpression of MaSip2 in M. alpina significantly promoted glucose uptake and resulted in 34.1% increased total biomass, leading to 44.8% increased arachidonic acid yield after 7 day fermentation. MaSip2 also regulated the balance between polyunsaturated fatty acids and carbohydrates in M. alpina. Intracellular metabolite analysis revealed increased carbohydrate-related metabolite accumulation in MaSip2 overexpression strains. On the contrary, knockdown of MaSip2 increased the total fatty acid unsaturation degree, especially under low-temperature conditions. This research improved our knowledge of SNF1 complex in M. alpina and provided a target gene for enhancing glucose utilization and modulating fatty acid composition for better application of oleaginous fungi.

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Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects

Cited by 46 publications

References 209 publications

Prospect of metabolic engineering in enhanced microbial lipid production: review

Prospect of metabolic engineering in enhanced microbial lipid production: review

Autophagy Improves ARA-Rich TAG Accumulation in Mortierella alpina by Regulating Resource Allocation

SNF1β-Modulated Glucose Uptake and the Balance between Polyunsaturated Fatty Acids and Carbohydrates in Mortierella alpina

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