Lipid methodology.

Cramp, D.G.

doi:10.1136/jcp.s1-5.1.17

Cited by 1 publication

(1 citation statement)

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“…The samples were placed in a 96 well plate for lipid quantification, using established spectrophotometric methods [19,20] . Spectrophotometric absorbancies of each cell lysate sample were compared to known lipid standards, and the concentrations of cholesterol and triglyceride were calculated from the derived measurements [19,20] . Protein was quantified in triplicate utilizing samples from the NaOH suspension, employing standard spectrophotometric methods [21] .…”

Section: Protein and Lipid Analysesmentioning

confidence: 99%

Lipogenesis in Huh7 cells is promoted by increasing the fructose: Glucose molar ratio

Windemuller

Rabinowitz

et al. 2016

WJH

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AIM:To determine whether hepatocyte lipogenesis, in an in vitro cell culture model, is modulated by adjusting culture media monosaccharide content and concentration. METHODS:Hepatocytes (Huh7), demonstrating glucose and fructose uptake and lipid biosynthesis, were incubated in culture media containing either glucose alone (0.65-0.72 mmol/L) or isosmolar monosaccharide (0.72 mmol/L) comprising fructose:glucose (F:G) molar ratios ranging from 0.58-0.67. Following a 24-h incubation, cells were harvested and analyzed for total protein, triglyceride (TG) and cholesterol (C) content. Significant differences (P < 0.05) among groups were determined using analysis of variance followed by Dunnett's test for multiple comparisons. RESULTS:After a 24 h incubation period, Huh7 cell mass and viability among all experimental groups were not different. Hepatocytes cultured with increasing concentrations of glucose alone did not demonstrate a significant change either in C or in TG content. However, when the culture media contained increasing F: G molar ratios, at a constant total monosaccharide

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Section: Protein and Lipid Analysesmentioning

confidence: 99%