Lipid Monolayer Formation and Lipid Exchange Monitored by a Graphene Field-Effect Transistor

Blaschke, Benno M.; Böhm, Philip; Drieschner, Simon; Nickel, Bert; Garrido, José Antonio

doi:10.1021/acs.langmuir.8b00162

Cited by 16 publications

(16 citation statements)

References 86 publications

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“…We prepared a vesicle suspension stock by sonicating commercial soybean lecithin (ca. 60% phospholipid, 35% lipid, with no known reductants or TrxR or NADPH) in water 77 , 78 , then incubated stock dilutions with Fast-TRFS and Linear-TRFS .…”

Section: Resultsmentioning

confidence: 99%

Cyclic 5-membered disulfides are not selective substrates of thioredoxin reductase, but are opened nonspecifically

et al. 2022

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The cyclic five-membered disulfide 1,2-dithiolane has been widely used in chemical biology and in redox probes. Contradictory reports have described it either as nonspecifically reduced in cells, or else as a highly specific substrate for thioredoxin reductase (TrxR). Here we show that 1,2-dithiolane probes, such as “TRFS” probes, are nonspecifically reduced by thiol reductants and redox-active proteins, and their cellular performance is barely affected by TrxR inhibition or knockout. Therefore, results of cellular imaging or inhibitor screening using 1,2-dithiolanes should not be interpreted as reflecting TrxR activity, and previous studies may need re-evaluation. To understand 1,2-dithiolanes’ complex behaviour, probe localisation, environment-dependent fluorescence, reduction-independent ring-opening polymerisation, and thiol-dependent cellular uptake must all be considered; particular caution is needed when co-applying thiophilic inhibitors. We present a general approach controlling against assay misinterpretation with reducible probes, to ensure future TrxR-targeted designs are robustly evaluated for selectivity, and to better orient future research.

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Section: Resultsmentioning

confidence: 99%

Cyclic 5-membered disulfides are not selective substrates of thioredoxin reductase, but are opened nonspecifically

et al. 2022

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“…We prepared a vesicle suspension stock by sonicating commercial soybean lecithin (ca. 60% phospholipid, 35% lipid, with no known reductants or TrxR or NADPH) in water 76,77 , then incubated stock dilutions with Fast-TRFS and Linear-TRFS.…”

Section: 2-dithiolane Probes Are Not Cellular Reporters Of Trxrmentioning

confidence: 99%

Cyclic 5-Membered Disulfides Are Not Selective Substrates of Thioredoxin Reductase, but Are Opened Nonspecifically

Felber¹,

Poczka²,

Scholzen³

et al. 2022

Preprint

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The cyclic five-membered disulfide 1,2-dithiolane has been used as the key element in numerous chemical biology probes. Contradictory views of this disulfide populate the literature: some reports describe it as being nonspecifically reduced, others as a highly specific substrate for thioredoxin reductase (TrxR). We here show that 1,2-dithiolane probes are nonspecifically reduced by a broad range of thiol reductants and redox-active proteins, and that their cellular performance is barely affected by TrxR inhibition or knockout. We conclude that inhibitor screenings and "TRFS" probes that have used 1,2-dithiolanes as TrxRselective substrates should be treated with caution, and may need re-evaluation. Understanding 1,2-dithiolanes’ behaviour needs consideration of probe localisation and environmentdependent fluorescence, reduction-independent ring-opening polymerisation, thiol-dependent cellular uptake, and caution when applying thiophilic inhibitors. We present an approach controlling against assay misinterpretation with reducible probes, to ensure that future TrxR-targeted designs are robustly evaluated for selectivity, and to better orient future research.

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“…The use of graphene-phospholipid complex is very promising in a wide range of biological applications [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. For example, phospholipid molecules coating graphene can enhance the antibacterial effect of laser-induced graphene [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…One of the most promising areas of application of graphene–lipid structures is biosensorics. Various biosensor devices have been developed based on lipid-modified graphene [ 26 , 27 , 28 ]. The urgent task in the framework of the development of this field is to improve the sensitivity and stability of the lipid/graphene system-based sensors.…”

Section: Introductionmentioning

confidence: 99%

Improving the Sensory Properties of Layered Phospholipid-Graphene Films Due to the Curvature of Graphene Layers

Слепченков

Glukhova

2020

Polymers

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This article is devoted to the in silico study of the sensory properties of mono- and bilayer phospholipid-graphene films with planar and curved graphene sheets. The DPPC (dipalmitoylphosphatidylcholine) molecules are considered as phospholipid structures. These molecules are part of lipid bilayers, liposomes and cell membranes. To find a way to improve the sensory properties of phospholipid-graphene films, we studied the effect of the curvature of the graphene sheet on the charge transfer and electrical conductivity of the films. The distribution of the electron charge density over the film atoms was calculated using the self-consistent-charge density-functional tight-binding method (SCC-DFTB). The calculation of the current through phospholipid-graphene films was carried out within the framework of the Landauer–Buttiker formalism using the Keldysh nonequilibrium Green function technique. As a result of the calculations, the optimal configuration of the arrangement of DPPC molecules between two graphene layers was established. This configuration provides the maximum possible increase in current to 1 μA at low voltages of ~0.2 V and is achieved for curved graphene with a radius of curvature of ~2.7 nm at individual points of graphene atomic network.

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Lipid Monolayer Formation and Lipid Exchange Monitored by a Graphene Field-Effect Transistor

Cited by 16 publications

References 86 publications

Cyclic 5-membered disulfides are not selective substrates of thioredoxin reductase, but are opened nonspecifically

Cyclic 5-membered disulfides are not selective substrates of thioredoxin reductase, but are opened nonspecifically

Cyclic 5-Membered Disulfides Are Not Selective Substrates of Thioredoxin Reductase, but Are Opened Nonspecifically

Improving the Sensory Properties of Layered Phospholipid-Graphene Films Due to the Curvature of Graphene Layers

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