Lipid monolayer spontaneous curvatures: A collection of published values

Dymond, Marcus K.

doi:10.1016/j.chemphyslip.2021.105117

Cited by 33 publications

(37 citation statements)

References 76 publications

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“…However, the in vitro reconstitution showed that nsP1 primarily binds monovalent anionic lipids ( Figure 3A–E ). Such lipids are reported to have near-zero or slightly positive spontaneous curvature, which excludes nsP1-induced lipid recruitment as a mechanism for stabilizing the spherule shape ( Dymond, 2021 ). Instead, we made the biological Ansatz that membrane remodeling is driven by the generation of dsRNA.…”

Section: Resultsmentioning

confidence: 99%

Architecture of the chikungunya virus replication organelle

Laurent

Kumar

Liese

et al. 2022

eLife

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Alphaviruses are mosquito-borne viruses that cause serious disease in humans and other mammals. Along with its mosquito vector, the Alphavirus chikungunya virus (CHIKV) has spread explosively in the last 20 years, and there is no approved treatment for chikungunya fever. On the plasma membrane of the infected cell, CHIKV generates dedicated organelles for viral RNA replication, so-called spherules. Whereas structures exist for several viral proteins that make up the spherule, the architecture of the full organelle is unknown. Here, we use cryo-electron tomography to image CHIKV spherules in their cellular context. This reveals that the viral protein nsP1 serves as a base for the assembly of a larger protein complex at the neck of the membrane bud. Biochemical assays show that the viral helicase-protease nsP2, while having no membrane affinity on its own, is recruited to membranes by nsP1. The tomograms further reveal that full-sized spherules contain a single copy of the viral genome in double-stranded form. Finally, we present a mathematical model that explains the membrane remodeling of the spherule in terms of the pressure exerted on the membrane by the polymerizing RNA, which provides a good agreement with the experimental data. The energy released by RNA polymerization is found to be sufficient to remodel the membrane to the characteristic spherule shape.

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Section: Resultsmentioning

confidence: 99%

Architecture of the chikungunya virus replication organelle

Laurent

Kumar

Liese

et al. 2022

eLife

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“…These data support a recent publication [2] where the possible antioxidant properties of supplements derived from plant extracts were measured using a targeted lipidomics approach. The motivation for the research was to understand how oxidative stress impacts membrane lipid composition [3] , collective membrane physical properties [4] and any ameliorating properties of dietary supplements in this context. Using lipidomics with complex lipid mixtures allows the isolated effects of lipid oxidation to be elucidated away from the lipid homeostatic responses of cells, which is particularly interesting since lipids are associated with many cellular processes and organelles through lipid protein or lipid DNA interactions [3 , 5 , 6] .…”

Section: Data Descriptionmentioning

confidence: 99%

Data for “Oxidative stress is inhibited by plant-based supplements: a quantitative lipidomic analysis of antioxidant activity and lipid compositional change”

2023

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“…Curvature induced by differences in lipid abundance or intrinsic spontaneous curvatures ( c 0 ) of lipids across the two leaflets of the bilayer can be modeled as a net membrane spontaneous curvature C 0 . PL composition is a major determinant of c 0 , which ranges from near zero for cylindrical lipids like PC, to negative values for conical lipids like PE or CL, and positive in the case of lyso-PLs 86 . PL acyl composition also modulates c 0 , with more voluminous unsaturated chains favoring negative spontaneous curvatures 87 .…”

Section: Introductionmentioning

confidence: 99%

Cristae formation is a mechanical buckling event controlled by the inner membrane lipidome

Venkatraman

Lee

García

et al. 2023

Preprint

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The inner mitochondrial membrane (IMM) is the site of bulk ATP generation in cells and has a broadly conserved lipid composition enriched in unsaturated phospholipids and cardiolipin (CL). While proteins that shape the IMM and its characteristic cristae membranes (CM) have been defined, specific mechanisms by which mitochondrial lipids dictate its structure and function have yet to be elucidated. Here we combine experimental lipidome dissection with multi-scale modeling to investigate how lipid interactions shape CM morphology and ATP generation. When modulating fatty acid unsaturation in engineered yeast strains, we observed that loss of di-unsaturated phospholipids (PLs) led to a breakpoint in IMM topology and respiratory capacity. We found that PL unsaturation modulates the organization of ATP synthases that shape cristae ridges. Based on molecular modeling of mitochondrial-specific membrane adaptations, we hypothesized that conical lipids like CL buffer against the effects of saturation on the IMM. In cells, we discovered that loss of CL collapses the IMM at intermediate levels of PL saturation, an effect that is independent of ATP synthase oligomerization. To explain this interaction, we employed a continuum modeling approach, finding that lipid and protein-mediated curvatures are predicted to act in concert to form curved membranes in the IMM. The model highlighted a snapthrough instability in cristae tubule formation, which could drive IMM collapse upon small changes in composition. The interaction between CL and di-unsaturated PLs suggests that growth conditions that alter the fatty acid pool, such as oxygen availability, could define CL function. While loss of CL only has a minimal phenotype under standard laboratory conditions, we show that its synthesis is essential under microaerobic conditions that better mimic natural yeast fermentation. Lipid and protein-mediated mechanisms of curvature generation can thus act together to support mitochondrial architecture under changing environments.

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Lipid monolayer spontaneous curvatures: A collection of published values

Cited by 33 publications

References 76 publications

Architecture of the chikungunya virus replication organelle

Architecture of the chikungunya virus replication organelle

Data for “Oxidative stress is inhibited by plant-based supplements: a quantitative lipidomic analysis of antioxidant activity and lipid compositional change”

Cristae formation is a mechanical buckling event controlled by the inner membrane lipidome

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