Lipid nanodomains change ion channel function

Weinrich, Michael; Worcester, David L.; Bezrukov, Sergey M.

doi:10.1039/c7nr03926c

Cited by 27 publications

(19 citation statements)

References 41 publications

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“…Thus, such phase changes could affect ion channels which are sensitive to lipid composition. We recently demonstrated that gramicidin, a model ion channel, has a much shorter lifetime in the ordered phase of a lipid-raft mixture, and that phase mixing shifts the distribution of lifetimes (Weinrich et al, 2017). Fig.…”

Section: Discussionmentioning

confidence: 97%

The actions of volatile anesthetics: a new perspective

Weinrich

Worcester

2018

Acta Crystallogr D Struct Biol

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Section: Discussionmentioning

confidence: 97%

The actions of volatile anesthetics: a new perspective

Weinrich

Worcester

2018

Acta Crystallogr D Struct Biol

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“…Conductance measurements were performed as described previously (59) For data analysis by Clampfit 10.3, no digital filtering was applied. Individual events of current blockages were discriminated and kinetic parameters were acquired by fitting logarithmic single, double, or triple exponentials to logarithmically binned histograms (60) as described previously (61). All lifetime histograms used 10 bins per decade.…”

Section: Expression and Purification Of Recombinantmentioning

confidence: 99%

Sequence diversity of tubulin isotypes in regulation of the mitochondrial voltage-dependent anion channel

Rostovtseva

Gurnev

Hoogerheide

et al. 2018

Journal of Biological Chemistry

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The microtubule protein tubulin is a heterodimer comprising / subunits, in which each subunit features multiple isotypes in vertebrates. For example, seven -tubulin and eight -tubulin isotypes in the human tubulin gene family vary mostly in the length and primary sequence of the disordered anionic C-terminal tails (CTTs). The biological reason for such sequence diversity remains a topic of vigorous enquiry. Here, we demonstrate that it may be a key feature of tubulin's role in regulation of the permeability of the mitochondrial outer membrane voltagedependent anion channel (VDAC). Using recombinant yeast /-tubulin constructs with -CTTs, -CTTs, or both from various human tubulin isotypes, we probed their interactions with VDAC reconstituted into planar lipid bilayers. A comparative study of the blockage kinetics revealed that either -CTTs or -CTTs block VDAC pore and that the efficiency of blockage by individual CTTs spans two orders of magnitude, depending on the CTT isotype.-Tubulin constructs, notably 3, blocked VDAC most effectively. We quantitatively describe these experimental results using a physical model that accounts only for the number and distribution of charges in the CTT, and not for the interactions between specific residues on the CTT and VDAC pore. Based on these results, we speculate that the effectiveness of VDAC regulation by tubulin depends on the predominant tubulin isotype in a cell. Consequently, the fluxes of ATP/ADP http://www.jbc.org/cgi

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“…30,32 These carotenoid-rich nanodomains in MLVs are instrumental in modulating membrane thermodynamics, which is parallel to lipid nanodomains in biological membranes, that are functional for facilitating membrane-associated cellular processes. 33,34 Polar carotenoids decrease fluidity and water permeability of the hydrophobic regions in lipid bilayers Membrane fluidity was probed at different regions of the lipid bilayer using Prodan and Laurdan, where Prodan probes at the headgroups (hydrophilic region) and Laurdan probes at the hydrophobic region given its longer hydrocarbon chain (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%