Lipid nanoparticles containing oryzalin for the treatment of leishmaniasis

Lopes, Rodrigo J.G.; Eleutério, Carla; Gonçalves, Lídia; Cruz, M.E.M.; Almeida, António J.

doi:10.1016/j.ejps.2011.09.017

Cited by 91 publications

(60 citation statements)

References 45 publications

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“…The cytotoxicity was assessed in HaCaT cells (CLS, Germany), a spontaneously immortalized human keratinocyte cell line, using a general cell viability endpoint MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) reduction assay (Cadete et al, 2012;Lopes et al, 2012). Inhibitory concentrations (IC50) were calculated using GraphPad Prism software v5.0 (GraphPad Software, Inc., La Jolla, CA) by the sigmoidal curve fitting method.…”

Section: In Vitro Studies Of Sunscreen Formulationsmentioning

confidence: 99%

Melatonin-based pickering emulsion for skin's photoprotection

et al. 2016

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Context: Based on its antioxidant activity, melatonin was recently found to have a protection effect against photocarcinogenesis. Objective: This work aimed to develop an innovative sunscreen formulation based on the Pickering emulsions concept, stabilized by physical UV filters, modified starch and natural oils associated to melatonin as a key strategy for prevention against UV-induced skin damage. Materials and methods: For this purpose, melatonin was incorporated in Pickering emulsions that were characterized using physicochemical, in vitro and in vivo testing. Physicochemical studies included physical and chemical stability by a thorough pharmaceutical control. The possible protective effects of melatonin against UV-induced cell damage in HaCaT cell lines were investigated in vitro. The safety assessment and the in vivo biological properties of the final formulations, including Human Repeat Insult Patch Test and sunscreen water resistance tests were also evaluated. Results and discussion: These studies demonstrated that melatonin sunscreen Pickering emulsion was beneficial and presented a powerful protection against UVB-induced damage in HaCat cells, including inhibition of apoptosis. The inclusion of zinc oxide, titanium dioxide, green coffee oil and starch ensured a high SPF (50+) against UVA and UVB. Conclusion: The combination of melatonin, multifunctional solid particles and green coffee oil, contributed to achieve a stable, effective and innovative sunscreen with a meaningful synergistic protection against oxidative stress.

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Section: In Vitro Studies Of Sunscreen Formulationsmentioning

confidence: 99%

Melatonin-based pickering emulsion for skin's photoprotection

et al. 2016

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“…SLN was prepared using either the emulsification-solvent evaporation (ESE) or the hot high-shear homogenization (HH) techniques, as previously described by Gaspar et al [20] and Lopes et al [27], respectively. Briefly, for the first method, the lipid (COM or PRE, 50 mg), alone or mixed with SA (10 mg) was dissolved in dichloromethane, and then added dropwise to the aqueous phase containing Tween 20 (5 mg/mL) and SDC (6 mg/mL) and sonicated (40 W, 2.5 min, Branson Sonifier 250, USA).…”

Section: Preparation Of Slnmentioning

confidence: 99%

“…It has been claimed that SLN should overcome some of the major disadvantages of other colloidal carriers, such as in particular the low stability and possible biotoxicity [31,30], and offer several advantages such as high drug loading, protection from degradation and release modulation [1,50,9,30]. Many studies showed that SLN is actually able to prolong and/or control drug delivery, enhance its stability and improve its bioavailability [27,49,60,38,19]. In particular, the SLN effectiveness in improving the oral bioavailability of poorly-soluble drugs is mainly attributed to the nanoscale dimensions of the particles combined with the almost molecular dispersion of the drug molecules into the lipid carrier and the presence of surfactants, which strongly increases the drug gastrointestinal solubilization, and the in vivo absorption [15,40,54].…”

Section: Introductionmentioning

confidence: 99%

Development of solid lipid nanoparticles as carriers for improving oral bioavailability of glibenclamide

Gonçalves

Maestrelli

Mannelli

et al. 2016

European Journal of Pharmaceutics and Biopharmaceutics

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a b s t r a c tA solid lipid nanoparticle (SLN) formulation was developed with the aim of improving the oral bioavailability and the therapeutic effectiveness of glibenclamide (GLI), a poorly water-soluble drug used in the treatment of type 2 diabetes. The SLN was prepared using different lipid components (Precirol Ò and Compritol Ò) and preparation procedures. Precirol-based SLN, obtained with the emulsion of solvent evaporation technique gave the best results and was selected for drug loading. Addition of lecithin to the SLN core or PEG coating was effective in increasing the nanoparticles stability in simulated gastric solution. Both such formulations were stable after one month storage at 5 ± 3°C, exhibited the absence of in vitro cytotoxicity, and presented a similar in vitro prolonged-release, reaching 100% release after 24 h. The lecithin-containing GLI-loaded SLN formulation, selected for in vivo studies in virtue of its higher EE% than the PEG-coated formulation (70.3% vs 19.6%), showed a significantly stronger hypoglycemic effect with respect to the drug alone, in terms of both shorter onset time and longer duration of the effect. These positive results indicated that the proposed SLN approach was successful in improving GLI oral bioavailability, confirming its potential as an effective delivery system for a suitable therapy of diabetes.

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“…Therefore, macrophages in bone marrow as well as in the liver and spleen are target cells in leishmaniasis treatment. Thus several nanoparticles loaded with therapeutic agents such as nanoparticles loaded with amphotericin B (Gupta & Vyas 2007;Nahar et al, 2010 ), PLGA nanoparticles loaded with saponin ( Van de Ven et al, 2012), lipid nanoparticles loaded with oryzalin (Lopes et al, 2012), PLGA nanoparticles loaded with kinetoplastid membrane protein-11 (Santos et al, 2012) have been developed to deliver the drugs in leishmania-infected macrophages. Drug carriers targeting bone marrow, especially bone marrow macrophages, would have a great potential to deliver these therapeutic agents in leishmania-infected macrophages.…”

Section: Future Prospects For Bone-marrow-targeted Drug Carrier Systemsmentioning

confidence: 99%

Advanced Drug Carriers Targeting Bone Marrow

Sou¹

2012

Recent Advances in Novel Drug Carrier Systems

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Lipid nanoparticles containing oryzalin for the treatment of leishmaniasis

Cited by 91 publications

References 45 publications

Melatonin-based pickering emulsion for skin's photoprotection

Melatonin-based pickering emulsion for skin's photoprotection

Development of solid lipid nanoparticles as carriers for improving oral bioavailability of glibenclamide

Advanced Drug Carriers Targeting Bone Marrow

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