Lipid Peroxidation and Antioxidant Activities of the Aqueous Rhizome Extract of Rheum officinale Baillon

Abstract

Generation of reactive oxygen species (ROS) is associated with dysregulation of antioxidant defense mechanisms and incidence of human diseases. e speci c aim of this study was to investigate the lipid oxidation and antioxidant activity of aqueous extract of Rheum o cinale Baillon rhizome in order to evaluate its potential as a future novel natural antioxidant resource and a functional ingredient in food and pharmaceutical formations. Total phenolic and avonoid contents of Rheum rhizome extract were dose dependently increased. Consistent with this, radical scavenging activities of Rheum rhizome extract as determined by 2,2-diphenyl-1-picrylhydrazyl assay and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity were signi cantly elevated as the concentration increased. In addition, the treatment of aqueous Rheum rhizome extract signi cantly increased ferric reducing and copper chelating activities. According to results of thiobarbituric acid reactive substance analysis, Rheum rhizome extract signi cantly delayed lipid oxidation. Preincubation with Rheum rhizome extract signi cantly inhibited tert-butyl hydroperoxide-(t-BHP-) induced ROS generation. Moreover, superoxide anion production was signi cantly lower in Rheum rhizome extract-treated RAW264.7 macrophage cells than t-BHP-incubated cells (p < 0.05). ese ndings suggest that Rheum o cinale Baillon rhizome extract has a potential as an excellent natural antioxidant agent.

Ameliorative effect of a standardized polyherbal combination in methotrexate-induced nephrotoxicity in the rat

“…Our developed polyherbal combination does ameliorate oxidative markers. Previous reports of reduction in the oxidative markers with B. diffusa (Pareta et al 2011b), R. emodi (Chang and Kim 2018), N. nucifera (Krishnamoorthy et al 2009) and C. nurvala (Shirwaikar et al 2004) are in line with the current findings. The antioxidant effect of the constituent ingredients seems to contribute to the overall nephroprotective action of the polyherbal combination.…”

Phytochemical screening and evaluation of the antioxidant and anti-bacterial activity of Woundwort (Anthyllis vulneraria L.)

“…According to several researches that studied the positive impact of polyphenol consumption on health and prevention of diseases, manufacturers are now marketing polyphenol-enriched foods and dietary supplements (Martin and Apple 2009). In addition, their antioxidant activity ensures better preservation of cosmetic and food products by preventing lipid peroxidation (Chang and Kim 2018). In cosmetic industry, phenolic compounds are added in cosmetic products owing to their well-recognised properties, such as antioxidant, anti-inflammatory, antimicrobial, antimutagens and anti-aging activities, as well as their emollients, humectant, wound healing, protective agents against UV-B damage, and reducing skin discoloration effects (Halla et al 2018).…”

Bioinorganic Chemistry and Computational Study of Herbal Medicine to Treatment of Tuberculosis

“…The pKa value of most phenols is around 9.0-10.0, but in the presence of cations such as Fe(III) or Cu(II), the proton is donated at physiological pH 5.0-8.0 [52]. The ability of phenol in metal chelation can inhibit lipid peroxidation by binding metal ions to stable complex forms or preventing the interaction of metal ions with lipid hydroperoxides, which are consistently produced in living cells [53,54]. The scavenger potencies of flavonoid metal complexes were significantly higher than those of the parent flavonoids.…”