Lipid peroxidation and antioxidants status in human malignant and non-malignant thyroid tumours

Stanley, J. P.; Neelamohan, R; Suthagar, E.; Ganapathy, Vengatesh; Jayakumar, Jay; Chandrasekaran, M; Banu, SK; Aruldhas, M. M.

doi:10.1177/0960327115597982

Cited by 21 publications

(12 citation statements)

References 24 publications

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“…Hyperthyroid rats were reported to have significantly higher lipid peroxidation, SOD, CAT, and GPx in liver, together with lower serum antioxidant status (Messarah et al, 2010). It was recently reported that changes in lipid peroxidation and SOD between normal and PTC thyroid tissues can be used as possible markers to differentiate between malignant and non-malignant thyroid tumours (Stanley et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

Analyses of antioxidant status and nucleotide alterations in genes encoding antioxidant enzymes in patients with benign and malignant thyroid disorders

Ramli

Junit

Leong

et al. 2017

PeerJ

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BackgroundSynthesis of thyroid hormones and regulation of their metabolism involve free radicals that may affect redox balance in the body. Thyroid disorders causing variations in the levels of thyroid hormones may alter cellular oxidative stress. The aim of this study was to measure the antioxidant activities and biomarkers of oxidative stress in serum and red blood cells (RBC) of patients with benign and malignant thyroid disorders and to investigate if changes in the antioxidant activities in these patients were linked to alterations in genes encoding the antioxidant enzymes.MethodsForty-one patients with thyroid disorders from University of Malaya Medical Centre were recruited. They were categorised into four groups: multinodular goitre (MNG) (n = 18), follicular thyroid adenoma (FTA) (n = 7), papillary thyroid cancer (PTC) (n = 10), and follicular thyroid cancer (FTC) (n = 6). Serum and RBC of patients were analysed for antioxidant activities, antioxidant enzymes, and biomarkers of oxidative stress. Alterations in genes encoding the antioxidant enzymes were analysed using whole exome sequencing and PCR–DNA sequencing.ResultsPatients with thyroid disorders had significantly higher serum superoxide dismutase (SOD) and catalase (CAT) activities compared to control, but had lower activities in RBC. There were no significant changes in serum glutathione peroxidase (GPx) activity. Meanwhile, GPx activity in RBC was reduced in PTC and FTC, compared to control and the respective benign groups. Antioxidant activities in serum were decreased in the thyroid disorder groups when compared to the control group. The levels of malondialdehyde (MDA) were elevated in the serum of FTA group when compared to controls, while in the RBC, only the MNG and PTC groups showed higher MDA equivalents than control. Serum reactive oxygen species (ROS) levels in PTC group of both serum and RBC were significantly higher than control group. Whole exome sequencing has resulted in identification of 49 single nucleotide polymorphisms (SNPs) in MNG and PTC patients and their genotypic and allelic frequencies were calculated. Analyses of the relationship between serum enzyme activities and the total SNPs identified in both groups revealed no correlation.DiscussionDifferent forms of thyroid disorders influence the levels of antioxidant status in the serum and RBC of these patients, implying varying capability of preventing oxidative stress. A more comprehensive study with a larger target population should be done in order to further evaluate the relationships between antioxidant enzymes gene polymorphisms and thyroid disorders, as well as strengthening the minor evidences provided in literatures.

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Section: Discussionmentioning

confidence: 99%

Analyses of antioxidant status and nucleotide alterations in genes encoding antioxidant enzymes in patients with benign and malignant thyroid disorders

Ramli

Junit

Leong

et al. 2017

PeerJ

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“…Hypothyroidism and hyperthyroidism could modify the oxidant-antioxidant balance in the serum and tissues [21]. There are also numerous reports on oxidative stress and thyroid states, especially thyroid cancer, although the mechanisms are still unknown [22–25]. In this study, HYD-H and HYD-Y affected the oxidative and antioxidative status of the organism by decreasing or increasing the serum levels of MDA in goiter rats.…”

Section: Discussionmentioning

confidence: 77%

Do Different Species of Sargassum in Haizao Yuhu Decoction Cause Different Effects in a Rat Goiter Model?

Liu

Chen

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Sargassum species combined with Glycyrrhiza uralensis is a famous herbal pair in traditional Chinese medicine, as one of the so-called “eighteen antagonistic medicaments.” In the Chinese Pharmacopoeia, two different species of Sargassum, Sargassum pallidum and Sargassum fusiforme, are recorded but they are not clearly differentiated in clinical use. In this study, we aimed to determine whether the two species of Sargassum could result in different effects when combined with G. uralensis in Haizao Yuhu Decoction (HYD), which is used for treating thyroid-related diseases, especially goiter. HYD containing S. pallidum or S. fusiforme was administered to rats with propylthiouracil-induced goiter. After 4 weeks, pathological changes in the thyroid tissue and the relative thyroid weight indicated that HYD containing S. pallidum or S. fusiforme protected thyroid tissues from propylthiouracil damage. Neither species increased the propylthiouracil-induced decrease in serum levels of thyroid hormones. However, there were some differences in their actions, and only HYD containing S. fusiforme abated the propylthiouracil-induced elevation of serum thyroid-stimulating hormone levels and activated thyroglobulin mRNA expression.

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“…Several decades ago, high concentrations of hydrogen peroxide were detected on the thyrocyte apical surface, where thyroid hormone biosynthesis takes place, and later the enzymatic source of hydrogen peroxide involved in thyroid hormone biosynthesis was characterized by cloning of genes encoding NADPH oxidases: dual oxidases 1 and 2 (DUOX1 and DUOX2) (Carvalho and Dupuy 2013). Besides its physiological role, oxidative stress appears to accompany thyroid carcinogenesis and to influence disease progression (Young et al 2010;Stanley et al 2016;Ece et al 2013;Yanagawa et al 1999). Damaging effect in thyroid tissue occur when there is the regulation misbalance between the produced free radicals and antioxidants, leading to apoptosis via activation of apoptosis signal-regulating kinase 1 (ASK1) (Du et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Papillary Thyroid Carcinoma: A Malignant Tumor with Increased Antioxidant Defense Capacity

Rovčanin

Gopčević

Kekić

et al. 2016

Tohoku J. Exp. Med.

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Papillary thyroid carcinoma (PTC) is the commonest thyroid malignancy worldwide for which the radiation exposure is the most influential risk factor. The levels of oxidative stress in PTC are not well characterized on the tissue level. The objective of this study was to evaluate total oxidant status (TOS) and total antioxidant status (TAS) in PTC and benign goiter (BG) tissues and to examine their association with clinicopathological characteristics. Tumor and normal thyroid tissue samples were collected from 59 PTC patients, and goiter tissues were collected from 50 BG patients. TOS and TAS were quantified in the tissue homogenates by spectrophotometric assays. TOS values in tumor tissues did not differ significantly from normal and goiter tissues; however, PTC tissues have significantly higher TAS values than normal and goiter tissues. TOS values correlated with retrosternal growth in BG patients. The significant correlations were found between TOS and TAS values and thyroid function parameters. In 17 PTC patients with multiple tumor foci (multicentric phenotype), TAS values were significantly lower, compared to 42 patients with unicentric PTC. TAS and TOS are the most useful predictors of thyroid capsular invasion by PTC. The age, sex, body mass index, smoking, familial history of thyroid disease and nodule size did not influence TOS and TAS in PTC or BG patients. In conclusion, we show the profiles of TOS and TAS in PTC and BG tissues. Importantly, PTC tissues possess increased antioxidant capacity. The redox status influences the parameters of the thyroid function and tumor's biological behavior.

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Lipid peroxidation and antioxidants status in human malignant and non-malignant thyroid tumours

Cited by 21 publications

References 24 publications

Analyses of antioxidant status and nucleotide alterations in genes encoding antioxidant enzymes in patients with benign and malignant thyroid disorders

Analyses of antioxidant status and nucleotide alterations in genes encoding antioxidant enzymes in patients with benign and malignant thyroid disorders

Do Different Species of Sargassum in Haizao Yuhu Decoction Cause Different Effects in a Rat Goiter Model?

Papillary Thyroid Carcinoma: A Malignant Tumor with Increased Antioxidant Defense Capacity

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