Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training

Alessio, Helaine M.; Goldfarb, Allan H.

doi:10.1152/jappl.1988.64.4.1333

Cited by 317 publications

(189 citation statements)

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“…Catalase With only a few exceptions (29,74,119,120), most studies report no significant alteration in CAT activity with acute exercise or chronic training (8,13). With a catalytic mechanism similar to SOD, CAT activity might be expected to increase during exercise due to increased H202 production.…”

Section: Antioxidant Enzymesmentioning

confidence: 99%

Exercise and oxidative stress: Sources of free radicals and their impact on antioxidant systems

Leichtweis

1997

AGE

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Strenuous exercise is characterized by increased oxygen consumption and the disturbance between intracellular pro-oxidant and antioxidant homeostasis. At lease three biochemical pathways (i.e., mitochondrial electron transport chain, xanthine oxidase, and polymorphoneutrophil) have been identified as potential sources of intracellular free radical generation during exercise. These deleterious reactive oxygen species pose a serious threat to the cellular antioxidant defense system, such as diminished reserves of antioxidant vitamins and glutathione. However, enzymatic and non-enzymatic antioxidants have demonstrated great versitility and adaptability in response to acute and chronic exercise. The delicate balance between pro-oxidants and antioxidants suggests that supplementation with antioxidants may be desirable for physically active individuals under certain physiological conditions by providing a larger protective margin.

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Section: Antioxidant Enzymesmentioning

confidence: 99%

Exercise and oxidative stress: Sources of free radicals and their impact on antioxidant systems

Leichtweis

1997

AGE

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“…Other studies report adaptations induced by endurance training in variables responsible for the improvement of oxidative metabolism such as a coordinated increase in the capacity for fatty acid, carbohydrate, ketone and amino acid oxidation and in the enzymatic pathways required for handling the reducing equivalents (9)(10)(11). Despite the beneficial adaptations induced by physical exercise, which leads to qualitative changes in muscle metabolism, an important side effect should be noted, such as the increased generation of reactive oxygen species (ROS), especially during exhaustive and unaccustomed exercise (12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Evaluation by blue native polyacrylamide electrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle

Molnár

Alves

Pereira-Da-Silva

et al. 2004

Braz J Med Biol Res

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Blue native polyacrylamide electrophoresis (BN-PAGE) is a technique developed for the analysis of membrane complexes. Combined with histochemical staining, it permits the analysis and quantification of the activities of mitochondrial oxidative phosphorylation enzymes using whole muscle homogenates, without the need to isolate muscle mitochondria. Mitochondrial complex activities were measured by emerging gels in a solution containing all specific substrates for NADH dehydrogenase and cytochrome c oxidase enzymes (complexes I and IV, respectively) and the colored bands obtained were measured by optique densitometry. The objective of the present study was the application of BN-PAGE colorimetric staining for enzymatic characterization of mitochondrial complexes I and IV in rat muscles with different morphological and biochemical properties. We also investigated these activities at different times after acute exercise of rat soleus muscle. Although having fewer mitochondria than oxidative muscles, white gastrocnemius muscle presented a significantly higher activity (26.7 ± 9.5) in terms of complex I/V ratio compared to the red gastrocnemius (3.8 ± 0.65, P < 0.05) and soleus (9.8 ± 0.9, P < 0.001) muscles. Furthermore, the complex IV/V ratio of white gastrocnemius muscle was always significantly higher when compared to the other muscles. Ninety-five minutes of exhaustive physical exercise induced a decrease in complex I/V and complex IV/V ratios after all resting times (0, 3 and 6 h) compared to control (P < 0.05), probably reflecting the oxidative damage due to increasing free radical production in mitochondria. These results demonstrate the possible and useful application of BN-PAGE-histochemical staining to physical exercise studies. Correspondence

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“…Likewise, Alessio and Goldfarb (20) have shown that the training on treadmill in young rats increases the CS activity in the gastrocnemius twice more than in the control group.…”

Section: Discussionmentioning

confidence: 88%

Exercício físico regular diminui o estresse oxidativo pulmonar em ratos após exposição aguda ao carvão mineral

Pinho

Piazza

Tuon

et al. 2006

Rev Bras Med Esporte

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Several studies have pointed the regular low to moderate intensity physical exercise an important agent to combat the oxidant stress. The purpose of this study was to investigate the effect of the physical exercise in the pulmonary oxidant response after inhaling mineral coal dust. Twenty-four male Wistar rats (200-250 g) were randomly divided in two groups with their respective controls (trained, n = 6; non-trained, n = 6). All animals received mineral coal dust via tracheal instillation (saline 3 mg/0.5 ml, 3 days/ week for 3 weeks), or 0.5 ml of 0.9% saline solution. Forty-eight hours after the last instillation, the trained group was submitted to a progressive exercise program on treadmill for twelve weeks (up to 17 m.min , 10% inclination). Forty-eight hours after the last training session, every animal was killed by decapitation, and their lungs and soleus were surgically removed for later biochemical analysis. The citrate synthase activity was determined in the soleus muscle, and the damages on the lipids and proteins were assessed into the lungs by means of the TBARS concentration and by determining the carbonyl groups, respectively. The results show that the regular practice of physical exercises reduces significantly the present TBARS levels in trained rats, decreasing the oxidation levels in proteins of both groups compared to their respective controls. The results lead us to suggest that the regular physical exercise on treadmill is an agent capable to soften the pulmonary oxidant damages caused by inhaling mineral coal particles.

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Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training

Cited by 317 publications

References 0 publications

Exercise and oxidative stress: Sources of free radicals and their impact on antioxidant systems

Exercise and oxidative stress: Sources of free radicals and their impact on antioxidant systems

Evaluation by blue native polyacrylamide electrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle

Exercício físico regular diminui o estresse oxidativo pulmonar em ratos após exposição aguda ao carvão mineral

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