Lipid peroxidation products and antioxidant enzymes in red blood cells during normal and diabetic pregnancy

Carone, Domenico; Loverro, Giuseppe; Greco, Pantaleo; Capuano, Ferdinando; Selvaggi, Luigi

doi:10.1016/0028-2243(93)90021-4

Cited by 63 publications

(42 citation statements)

References 17 publications

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“…These observations are in contrast to some previous reports on GPX in diabetes where GPX showed either no change or paralleled SOD, both showed a reciprocal relationship with MDA or RBARS levels [19,32,37,38]. The unexpected increase in the GPX activities here can only be evaluated in the context of a full spectrum of the levels of antioxidant components.…”

Section: Discussioncontrasting

confidence: 99%

See 1 more Smart Citation

Antioxidant Enzymes in Gestational Diabetes: A Study on a Kuwaiti Population

Ff¹,

Dashti²,

Ht³

et al. 2014

Bioenergetics

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Objectives: We hypothesize that enzymatic antioxidants are significant contributors to antioxidant status in gestational diabetes (GDM) through scavenging of free radicals. Methods: We evaluated total antioxidant activity and the activities of two major physiological antioxidant enzymes: glutathione peroxidase (GPX) and superoxide dismutase (SOD) in peripheral blood of 22 pregnant Kuwaiti women in second trimester afflicted with GDM, 28 healthy pregnant women (also in 2nd trimester) and 27 healthy non-pregnant women. Enzyme activities were measured using spectrophotometric assays.Results: No significant differences were noted in total antioxidant activity in peripheral blood in the three cohorts; however, serum SOD activity was significantly decreased in blood of both GDM women (p<0.05) and healthy pregnant subjects (p<0.05) relative to the non-pregnant cohort. Conversely, the activity of GPX was significantly elevated in blood of GDM-afflicted women relative to non-pregnant (p<0.001); and healthy pregnant women (p<0.001). GPX/SOD ratio was significantly higher in the GDM group compared to normal pregnancy and nonpregnant control (P<0.01). Conclusion:The activity of GPX /SOD ratio may be a marker of glycemic control in women with gestational diabetes. It is reasonable to conclude that the pathogenesis of GDM may involve the release of substantial quantities of mediators. This phenomenon suggest further investigation in the management of GDM which may involve other therapeutic targets for pharmacological intervention.

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Section: Discussioncontrasting

confidence: 99%

“…Unfortunately, controversial information has been reported on the intervention by antioxidant defense system. Carine et al [19] and Zachara et al [20] found no difference in GPX levels between pregnant women at third trimester and non-pregnant women. Conversely, Hchne and Walters [21] found increased GPX throughout pregnancy.…”

Section: Antioxidant Defense Systemmentioning

confidence: 95%

Antioxidant Enzymes in Gestational Diabetes: A Study on a Kuwaiti Population

Ff¹,

Dashti²,

Ht³

et al. 2014

Bioenergetics

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“…The plasma MDA concentrations in pregnant women in the third trimester were significantly higher than in the non-pregnant women (Arikan et al 2001;Djordjevic et al 2004;Nakai et al 2000). Peroxidation in the third trimester was significantly higher than in the first and second trimesters (Carine et al 1993;Djordjevic et al 2004). Pregnancy is characterized by increased lipid peroxidation.…”

Section: Discussionmentioning

confidence: 77%

Changes in the Rate of Lipid Peroxidation in Plasma and Selected Blood Antioxidants before and during Pregnancy in Ewes

2009

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Pregnancy is a condition that favours oxidative stress. The aim of this study was to evaluate the oxidant and antioxidant status by measuring glutathione peroxidase (GSH-Px), catalase (CAT) activities and malondialdehyde (MDA) and glutathione (GSH) concentrations before and during pregnancy in ewes. Twelve healthy female and two healthy male Awassi sheep, aged 4-5 years, weighing approximately 50 -55 kg each, were used in the study. The ewes' blood samples were taken before synchronization and during pregnancy (on 25 th days of each month of pregnancy). Plasma MDA concentrations in the 2 nd and 3 rd months of pregnancy in ewes were lower than in the 1 st , 4 th , 5 th months of pregnancy and in the non-pregnant ewes. GSH concentrations and GSH-Px activities during pregnancy were increased (P < 0.01). GSH concentrations and GSH-Px activities were the highest in the 2 nd and 3 rd months of pregnancy. CAT activities were decreased after the 1 st month of pregnancy in ewes (P < 0.001). They were the lowest in the 2 nd and 3 rd months of pregnancy. The findings of our study show decreased CAT activities and elevated GSH concentrations and GSH-Px activities after the 1 st month of pregnancy in ewes. These changes in the indicators under study may indicate a predisposition to oxidative stress in the 2 nd and 3 rd month of pregnancy in ewes. Awassi sheep, pregnancy, oxidant and antioxidant status

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“…Numerous studies have demonstrated higher concentrations of lipid peroxidation products in healthy pregnant women than in non-pregnant women (Uotila et al 1991, Qanungo et al 2000, Toescu at al 2002, Djordjevic et al 2004, Hung et al 2010, Idonije et al 2011. Some authors have found that plasma concentrations of TBARS and other markers of lipid peroxidation gradually increase during pregnancy, reaching the highest values in the third trimester (Carine et al 1993, Djordjevic et al 2004, Hung et al 2010), but other authors have reported that levels of the products of lipid peroxidation reach their maximum concentrations in the second trimester and then decline until term (Uotila et al 1991, Qanungo et al 2000. Also, a comparison of the levels of the markers of protein peroxidation processes in women indicates the occurrence of oxidative stress during pregnancy (Wisdom et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Assessment of lipid and protein peroxidation markers in non-pregnant and pregnant female dogs

Szczubiał¹,

Kankofer²,

Dąbrowski³

et al. 2015

Polish Journal of Veterinary Sciences

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The aim of the study was to investigate oxidative stress during normal pregnancy in female dogs based on an evaluation of plasma markers for lipid and protein peroxidation. Twenty clinically healthy female dogs (10 non-pregnant and 10 pregnant) were used in the study. Blood samples from the pregnant animals were collected at 19-21, 38-40, and 56-58 days of pregnancy. Blood samples from non-pregnant female dogs were obtained between 20 and 35 days after ineffective breeding. As indicators of oxidative stress, we measured the following using spectrophotometric and spectrofluorimetric methods: thiobarbituric acid reactive substances (TBARS), radical cations of N,N, diethylpara-phenylene diamine (RC-DEPPD), sulfhydryl groups (SH groups), bityrosine and formylkynurenine. The mean plasma TBARS concentration in the pregnant dogs (0.486 ± 0.071 -0.581 ± 0.191 μmol/g protein) was significantly higher (p<0.05) than that found in the non-pregnant animals (0.274 ± 0.111 μmol/g protein). A marked, although not significant, decrease in SH group content, as well as an increase in bityrosine and formylkynurenine concentration were concurrently observed in the pregnant dogs. No significant differences were found in terms of the studied markers in the pregnant animals when comparing the values obtained during the investigated periods of pregnancy, although there was a progressive decrease in TBARS concentration and a progressive increase in RC-DEPPD, bityrosine and formylkynurenine contents. Our findings suggest that normal pregnancy in female dogs is associated with oxidative stress. Further studies are necessary to establish the physiological ranges of antioxidative/oxidative profiles in pregnant dogs and to explain if and how the intensity of oxidative stress might contribute to the risk of the complications of pregnancy.

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Lipid peroxidation products and antioxidant enzymes in red blood cells during normal and diabetic pregnancy

Cited by 63 publications

References 17 publications

Antioxidant Enzymes in Gestational Diabetes: A Study on a Kuwaiti Population

Antioxidant Enzymes in Gestational Diabetes: A Study on a Kuwaiti Population

Changes in the Rate of Lipid Peroxidation in Plasma and Selected Blood Antioxidants before and during Pregnancy in Ewes

Assessment of lipid and protein peroxidation markers in non-pregnant and pregnant female dogs

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