Lipid Production by Pure and Mixed Cultures of Chlorella pyrenoidosa and Rhodotorula mucilaginosa Isolated in Nuevo Leon, Mexico

Abstract

Given the well-known environmental drawbacks of using fossil fuels, advances in the field of alternative energy have become a worldwide technological priority. Special interest has been focused on the production of biodiesel obtained from oleaginous microorganisms. In the present research, lipid production by two species, microalgae Chlorella pyrenoidosa and yeast Rhodotorula mucilaginosa was assessed, independently and in mixed culture to evaluate a possible synergy. Fatty acid analysis was performed by gas chromatography. Among pure and mixed cultures of both strains and several culturing conditions, the highest biomass and lipid productivity was obtained by C. pyrenoidosa (8.05 and 1.62 g/L, respectively). The results of this study showed that both strains used are in fact oleaginous strains as they were found to reach up to 20 % of lipids, in addition, lipids in both pure and mixed cultures were mainly of triglycerides (>90 %), composed of fatty acid chains between 16 and 18 carbons.

Oil-lipids, carotenoids and fatty acids simultaneous production by Rhodotorula mucilaginosa CCT3892 using sugarcane molasses as carbon source

“…Besides that, Silva et al (2018) obtained, using the same strain as this present study, a total lipids percentage of 13.33% using a cassava wastewater dilution (50% v v -1 ) as substrate. Reyna-Martínez et al (2015) reported that their best lipid accumulation was about 20% from a Rhotodotorula mucilaginosa cultivation at a C/N of 12.5, what is close to the results reported in this paper.…”

Antitumor activity of Chlorella sorokiniana and Scenedesmus sp. microalgae native of Nuevo León State, México

“…To our knowledge, this is the first report of Nuevo Leon, Mexico native microalgae, identified as C. sorokiniana and Scenedesmus sp., showing cytotoxicity against a murine lymphoma tumor cell line. Previous reports have shown microalgae potential for wastewater treatment and biodiesel production ( Reyna-Martínez et al, 2015 ; Beltrán-Rocha et al, 2017 ). In the present study, microalga isolates were grown under artificial light, closed photobioreactors, and previously established physicochemical conditions.…”

“…Biomass dried samples of C. sorokiniana and Scenedesmus sp. were placed in separate Whatman cellulose extraction thimbles (33 × 80 mm, thickness 1.5 mm) (Sigma-Aldrich, St. Louis, MO, USA) and placed in a Soxhlet extraction apparatus ( Reyna-Martínez et al, 2015 ), which is a continuous system consisting of a flat bottomed round flask, an extraction chamber with a siphon, and a condenser. This method was selected since this extraction is very practical and recommended by most of the methanol-soluble compounds for biological material recovering.…”

Produção de biomassa e lipídios pela levedura Rhodotorula mucilaginosa utilizando a manipueira como substrato

“…As biomassas produzidas no presente estudo são semelhantes às encontradas por Reyna-Martínez et al (2015), que produziram 6,27 g.L -1 quando cultivaram a levedura R. mucilaginosa em um meio sintético à base de glicose, extrato de levedura e peptona, e aos resultados de Cheng e Yang (2016), que obtiveram 6 g.L -1 de biomassa usando uma mistura de resíduos e melaço de cana, suplementados com extrato de levedura, como substrato.…”

“…São úteis também para a produção renovável e sustentável de óleos biodegradáveis com potencial para uso em gêneros alimentícios, combustíveis e produtos químicos (SITEPU et al, 2014). Para a produção de lipídios, devem-se escolher linhagens que apresentem uma melhor cinética de desenvolvimento, com uma maior velocidade de crescimento, necessitando de menores tempos de cultivo, que não produzam substâncias indesejáveis e que tenham a capacidade de metabolizar substratos de custo reduzido (POLI et al, 2013).…”