Lipid production from corn stover by a cost-efficient system featuring ammonium carbonate-steam explosion and recirculating enzymatic hydrolysis

Xu, Hui; Zhao, Ning; Yao, Huiyin; Qin, Han; Zeng, Jie; Ran, Yulu; Yang, Yanjun; Qiao, Dan; Cao, Yi

doi:10.1016/j.biombioe.2018.11.020

Cited by 14 publications

(12 citation statements)

References 34 publications

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“…The gravimetric data were in co-relation to confocal microscopy study (P < 0.05). The lipid productivity of R. pacifica INDKK in AS-PSH (0.058 g/L/h) was higher than the previously reported oleaginous yeasts on different lignocellulosic hydrolysates such as 0.041 g/L/h on waste office paper enzymatic hydrolysate [18], 0.02 g/L/h on saccharified sweet sorghum juice [46,47], and 0.029 g/L/h on corn stover enzymatic hydrolysate [48] as shown in Table 4. To the best of our knowledge, no yeast isolate reported has produced equivalent lipid titre to isolate R. pacifica INDKK on AS-PSH with effective glucose utilization rate.…”

Section: Discussionmentioning

confidence: 63%

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Kumar

Deeba

Sauraj

et al. 2020

Biotechnol Biofuels

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Background To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

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Section: Discussionmentioning

confidence: 63%

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Kumar

Deeba

Sauraj

et al. 2020

Biotechnol Biofuels

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“…SE combined with weak base reagents is an environmentally friendly and efficient pretreatment method, which effectively removes lignin and produces minimal amounts of inhibitors [15]. In this study, the CS is pretreated via an ammonium carbonate-steam explosion (AC-SE) according to previous research in the laboratory [16].…”

Section: Introductionmentioning

confidence: 99%

“…The yeast strain Saitozyma podozolica Zwy2-3, which was discovered and identified in early laboratory research, shows great potential for xylose metabolism and is a suitable candidate for SCO production [22]. Additionally, it can utilize mixed sugars obtained from EHCS-ACSE [16]. As an unconventional oleaginous yeast, most studies on the yeast strain of S. podzolica primarily utilize pure glucose or xylose as substrates [23,24].…”

Section: Introductionmentioning

confidence: 99%

Production of Microbial Lipids by Saitozyma podzolica Zwy2-3 Using Corn Straw Hydrolysate, the Analysis of Lipid Composition, and the Prediction of Biodiesel Properties

Feng,

Guo,

Ran

et al. 2023

Energies

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Although Saitozyma podzolica Zwy2-3 can use the enzymatic hydrolysate of corn stalks treated with an ammonium carbonate-steam explosion (EHCS-ACSE) as a substrate for lipid accumulation, the inefficient conversion of sugars from EHCS-ACSE into lipids necessitates the further optimization of fermentation parameters. Response surface design was used to optimize the primary fermentation parameters. Under the optimized conditions of the reducing sugar concentration of 89.44 g/L, yeast extract concentration of 3.88 g/L, rotational speed of 219 rpm, and incubation time of 122 h, the maximum lipid production achieved 11.45 g/L, which was 2.28 times higher than the results of the previous study. In addition, lipid profiling showed the presence of four fatty acid methyl esters, with the highest percentage being 61.84% oleic acid, followed by 21.53% palmitic acid, 13.05% stearic acid, and 3.58% linoleic acid. It is noteworthy that the composition and relative abundance of microbial lipids remained constant under different culture conditions. The characteristics of Zwy2-3 biodiesel, such as the iodine value (62.09), cetane number (59.29), density (0.87 g/cm3), and oxidation stability (35.53), meet the international standards (ASTM D6751-02 and EN 14214) for biodiesel. The present study further demonstrated that S. podzolica Zwy2-3 can efficiently utilize EHCS-ACSE for microbial lipid accumulation, and its lipids have favorable qualities that make them suitable for biodiesel production.

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“…In recent years, Saitozyma podzolica, a nonconventional oleaginous yeast of basidiomycetous species, has been discovered [6,7,8]. S. podzolica DSM 27192 could produce lipid and sugar acids from glucose or xylose, and its lipid accumulation is influenced by fermentation factors such as pH, temperature, and carbon sources concentration [7,9].…”

Section: Introductionmentioning

confidence: 99%

GATA-type Transcriptional Factor SpGAT1 Interacts with SpMIG1 and Promotes Lipid Accumulation in the Oleaginous Yeast Saitozyma podzolica zwy-2-3

Ran

Yang

et al. 2022

Preprint

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Background: In oleaginous yeast, nitrogen limitation is a critical parameter for lipid synthesis. GATA-family transcriptional factor GAT1, a member of the target of rapamycin (TOR) pathway and nitrogen catabolite repression (NCR), regulates nitrogen uptake and utilization. Therefore, it is significant to study the SpGAT1 regulatory mechanism of lipid metabolism for conversion of biomass to microbial oil in Saitozyma podzolica zwy-2-3. Results: Compared with WT, ∆gat1, and OE::gat1, the lipid yield of OE::gat1 increased markedly in the low carbon and nitrogen ratio (C/N ratio) mediums, while the lipid yield and residual sugar of ∆gat1 decreased in the high C/N ratio medium. According to yeast two-hybrid assays, SpGAT1 interacted with SpMIG1, and it's deletion drastically lowered SpMIG1 expression on the high C/N ratio medium. MIG1 deletion has been found in earlier research to affect glucose metabolic capacity, resulting in a prolonged lag period. Therefore, we speculated that SpGAT1 influenced glucose consumption rate across SpMIG1. Based on yeast one-hybrid assays and qRT-PCR analyses, SpGAT1 regulated the glyoxylate cycle genes ICL1, ICL2, and pyruvate bypass pathway gene ACS, irrespective of the C/N ratio. SpGAT1 also could bind to the ACAT2 promoter in the low C/N medium and induce sterol ester (SE) accumulation. Conclusion: Our findings indicated that SpGAT1 positively regulated lipid metabolism in S.podzolica zwy-2-3,but that its regulatory patterns varied depending on the C/N ratio. When the C/N ratio was high, SpGAT1 interacted with SpMIG1 to affect carbon absorption and utilization. SpGAT1 also stimulated lipid accumulation by regulating essential lipid anabolism genes. Our insights might spur more research into how nitrogen and carbon metabolism interact to regulate lipid metabolism.

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Lipid production from corn stover by a cost-efficient system featuring ammonium carbonate-steam explosion and recirculating enzymatic hydrolysis

Cited by 14 publications

References 34 publications

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Production of Microbial Lipids by Saitozyma podzolica Zwy2-3 Using Corn Straw Hydrolysate, the Analysis of Lipid Composition, and the Prediction of Biodiesel Properties

GATA-type Transcriptional Factor SpGAT1 Interacts with SpMIG1 and Promotes Lipid Accumulation in the Oleaginous Yeast Saitozyma podzolica zwy-2-3

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