Lipid Production from Hemicellulose and Holocellulose Hydrolysate of Palm Empty Fruit Bunches by Newly Isolated Oleaginous Yeasts

Tampitak, Srikanya; Louhasakul, Yasmi; Cheirsilp, Benjamas; Prasertsan, Poonsuk

doi:10.1007/s12010-015-1679-y

Cited by 28 publications

(17 citation statements)

References 30 publications

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“…5-HMF was less toxic than furfural and several species of oleaginous yeasts can grow even at high concentration of 5-HMF (2.5 g/L) [20,36]. Depending on the sources of biomass and types of hydrolysis employed, the concentration of these sugar derivatives in the lignocellulose hydrolysate can range between 0.5 and 11 g/L [18,37]. Figure 2b shows the effect of SYC loadings on the concentrations of total sugar, total nitrogen and C/N ratio in the hydrolysate.…”

Section: Methodsmentioning

confidence: 99%

“…The sugar concentration of BSG and SYC hydrolysates were adjusted to be the same at 40 g/L which was the concentration used for lipid production by the selected oleaginous yeasts [18] and the hydrolysate were added with 0.2 g/L MgSO 4 ·7H 2 O, 0.5 g/L KH 2 PO 4 and 0.1 g/L CaCl 2 ·2H 2 O. The initial pH was 6.0.…”

Section: Culture Conditionsmentioning

confidence: 99%

“…The concentrations of sugar derivatives were also determined. The hydrolysates with high sugar concentration were chosen as nutrient sources for lipid production by the potent oleaginous yeasts that could grow on industrial wastes [17][18][19] including Trichosporonoides spathulata JU4-57, Rhodotorula mucilaginosa G43 and Yarrowia lipolytica TISTR 5151.…”

Section: Introductionmentioning

confidence: 99%

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Acid Hydrolysis of Brewers’ Industrial Wastes and Their Use for Lipid Production by Oleaginous Yeasts

Sae-ngae

Cheirsilp

Suksaroj

et al. 2019

J. of Wat. & Envir. Tech.

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Brewers' spent grain (BSG) and spent yeast cell (SYC) are the wastes from mashing and fermentation process of beer. The compositions of BSG were 24.49 ± 0.91% hemicellulose, 20.86 ± 0.60% cellulose and 9.62 ± 0.40% lignin, while those of SYC were 46.97 ± 0.07% protein, 21.32 ± 0.08% carbohydrate and 5.73 ± 0.57% lipids. BSG and SYC were acid hydrolyzed using different solid loadings at 5-24%. The optimal solid loadings for BSG and SYC that gave maximum sugar concentration were 15% and 24%, respectively. Sugar compositions in BSG hydrolysate were 22.02 ± 0.8% glucose, 45.83 ± 1.53% xylose, and 32.13 ± 2.3% arabinose, while those found in SYC hydrolysate were 31.43 ± 0.38% glucose and 69.57 ± 1.04% mannose. Both hydrolysates were used as nutrient sources at the same sugar concentration of 40 g/L for cultivation of three oleaginous yeasts, Trichosporonoides spathulata JU4-57, Rhodotorula mucilaginosa G43 and Yarrowia lipolytica TISTR 5151. BSG hydrolysate gave higher biomass than SYC hydrolysate possibly due to a higher content of suitable sugars for cell growth. Among three yeasts, T. spathulate JU4-57 gave the highest lipid yields of 62.9 ± 5.67 and 39.9 ± 0.62 mg/g-substrate on BSG and SYC hydrolysates, respectively. This study may contribute greatly to low-cost production of oil sources for biodiesel production and may help to increase the environmental and economic sustainability of the brewery business.

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Section: Methodsmentioning

confidence: 99%

Section: Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Acid Hydrolysis of Brewers’ Industrial Wastes and Their Use for Lipid Production by Oleaginous Yeasts

Sae-ngae

Cheirsilp

Suksaroj

et al. 2019

J. of Wat. & Envir. Tech.

Self Cite

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“…Cultivation of C. starkeyi on bagasse hydrolysate under different culture modes had resulted in lipid yields not exceeding 28% [17]. In addition, C. tropicalis and T. cutaneum CX1 cultivated on palm empty fruit and corn stover hydrolysate, respectively, produce only 1.6 and 3.1 g/L of lipids using batch bioreactor culture [53,54]. However, the lipid yield of Y-MG1 was lower than that of R. mucilaginosa (6.64 g/L) using the nondetoxified wheat straw hydrolysate [19].…”

Section: Carbon Sourcementioning

confidence: 99%

Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis

Ayadi

Belghith

Gargouri

et al. 2019

BioMed Research International

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The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis. On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content. This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose. Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid. An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes. The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material. Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements. Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively. The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran. The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.

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“…Their strong oxidative metabolism enables the degradation of recalcitrant substrates, organochemicals and industrial wastes (Cheirsilp et al ., ; Johnson, ; Taskin, ). They are also naturally capable of bioconverting a variety of by‐products of the agrifood industry into added value primary and secondary metabolites and, based on that, have been proposed as a source of pigments and metabolites of interest in the food industry (Hernández‐Almanza et al ., ), as oil producers for biofuel application (Galafassi et al ., ; Li et al ., ; Tampitak et al ., ) and as enzyme producers (Canli et al ., ; Taskin, ). Moreover, these yeasts show antimicrobial activity against pathogenic fungi involved in postharvest diseases of fruit and vegetables (Li et al ., ; Zhang et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of Rhodotorula mucilaginosa: dealing with the issues of a non‐conventional yeast

Addis

Tanca

Landolfo

et al. 2016

Yeast

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Red yeasts ascribed to the species Rhodotorula mucilaginosa are gaining increasing attention, due to their numerous biotechnological applications, spanning carotenoid production, liquid bioremediation, heavy metal biotransformation and antifungal and plant growth-promoting actions, but also for their role as opportunistic pathogens. Nevertheless, their characterization at the 'omic' level is still scarce. Here, we applied different proteomic workflows to R. mucilaginosa with the aim of assessing their potential in generating information on proteins and functions of biotechnological interest, with a particular focus on the carotenogenic pathway. After optimization of protein extraction, we tested several gel-based (including 2D-DIGE) and gel-free sample preparation techniques, followed by tandem mass spectrometry analysis. Contextually, we evaluated different bioinformatic strategies for protein identification and interpretation of the biological significance of the dataset. When 2D-DIGE analysis was applied, not all spots returned a unambiguous identification and no carotenogenic enzymes were identified, even upon the application of different database search strategies. Then, the application of shotgun proteomic workflows with varying levels of sensitivity provided a picture of the information depth that can be reached with different analytical resources, and resulted in a plethora of information on R. mucilaginosa metabolism. However, also in these cases no proteins related to the carotenogenic pathway were identified, thus indicating that further improvements in sequence databases and functional annotations are strictly needed for increasing the outcome of proteomic analysis of this and other non-conventional yeasts.

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Lipid Production from Hemicellulose and Holocellulose Hydrolysate of Palm Empty Fruit Bunches by Newly Isolated Oleaginous Yeasts

Cited by 28 publications

References 30 publications

Acid Hydrolysis of Brewers’ Industrial Wastes and Their Use for Lipid Production by Oleaginous Yeasts

Acid Hydrolysis of Brewers’ Industrial Wastes and Their Use for Lipid Production by Oleaginous Yeasts

Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis

Proteomic analysis of Rhodotorula mucilaginosa: dealing with the issues of a non‐conventional yeast

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