Lipid Production From Waste Materials in Seawater-Based Medium by the Yeast Yarrowia lipolytica

Abstract

The global limitation of fossil fuels impels scientists to search for new energy sources. A good alternative is biodiesel produced from crop plants. However, its production requires huge quantities of farmland, fertilizers and fresh water, which is in conflict with the human demand for water for consumption and land for food production. Thus, production of single cell oil (SCO) by oleaginous microorganisms remains the best solution for the coming years. Whereas most microorganisms require fresh water for proper cell metabolism, in this study we demonstrate that the unconventional yeast Yarrowia lipolytica is able to produce huge quantities of fatty acid in seawater-based medium. Here we shown that Y. lipolytica is able to produce fatty acids in medium based on seawater and crude glycerol as the main carbon source, which allows for low-cost production of SCO, is beneficial for industrial application and is ecologically friendly.

Rye and Oat Agricultural Wastes as Substrate Candidates for Biomass Production of the Non-Conventional Yeast Yarrowia lipolytica

“…(IV) The lipid profile was examined by converting lipids into their methyl esters [17,29]. Fatty acids were identified and quantified with a Zebron ZB-FAME column installed in the Shimadzu single quadrupole GCMS-QP2010SE system with hexane as a solvent and helium as a carrier gas (linear velocity-35 cm/s).…”

“…It is considered as being generally recognized as safe (GRAS) by the U.S. Food and Drug Administration (FDA, USA), which is crucial when microorganisms are intended to be used in feed or food applications. Currently, the yeast Y. lipolytica is used in the synthesis of organic acids [14,15], in the synthesis of sweeteners [16], fatty acids [17,18], enzymes [19] and biomass [20]. Y. lipolytica is also used for production of single-cell protein [21].…”

Plant Power: Opportunities and challenges for meeting sustainable energy needs from the plant and fungal kingdoms

“…Saccharomyces cerevisiae, Mortierella isabellina, and the yeast Cystobasidium iriomotense (Tanimura et al, 2018) produce lipids, including from feedstock derived from plant biomass such as sweet sorghum, Sorghum bicolor (L.) Moench (Poaceae) (Economou, Aggelis, Pavlou, & Vayenas, 2011). The yeast Yarrowia lipolytica, can produce lipids from a seawater-based medium and crude glycerol (a by-product of biodiesel production) which could alleviate freshwater use for industrial purposes, such as growing media (Dobrowolski, Drzymała, Rzechonek, Mituła, & Mirończuk, 2019;Fukuda, Kondo, & Noda, 2001).…”

The influence of transketolase on lipid biosynthesis in the yeast Yarrowia lipolytica

“…After drying at 105 °C, the biomass was determined gravimetrically. The fatty acids (FAs) from lyophilized biomass were derivatized to fatty acid methyl esters (FAMEs) using the method described before [13]. FAMEs were analyzed by gas chromatography on GC-2010 Plus apparatus (Shimadzu, Japan) with a flame ionization detector (FID) and autoinjector (AOC-20i).…”