Lipid profile lowering effect of Soypro^TM fermented with lactic acid bacteria isolated from Kimchi in high‐fat diet‐induced obese rats

Abstract

Lactic acid bacteria are known to exert various physiologic functions in humans. In the current study, we investigated the effects of Soypro, a new soymilk fermented with lactic acid bacteria, like Leuconostoc kimchii, Leuconostoc citreum, and Lactobacillus plantarum, isolated from Kimchi, on adipocyte differentiation in preadipocyte 3T3-L1 cell lines and weight gain or the plasma lipid profile in Sprague-Dawley rats. Adipocyte 3T3-L1 cells treated with Soypro (10 microg/ml) significantly reduced the contents of cellular triglyceride and inhibited cell differentiation by Oil red O staining. Treatment with Soypro (10 microg/ml) for an additional two days in adipocytes inhibited the expression of peroxisome proliferator-activated receptor-gamma2 and CCAAT/enhancer binding protein-alpha, transcription factors of adipocyte differentiation. Based on these in vitro studies, we examined the anti-obesity effect of Soypro in rats for six weeks. Soypro had no significant effect on high-fat diet-induced increases in body weight, food intake, or feed gain ratio. However, the administration of Soypro significantly reduced the concentration of the plasma low density lipoprotein cholesterol. Changes in the plasma levels of total cholesterol and glucose were inclined to decrease in Soypro administrated groups compared with saline treated group. Triglyceride and high density lipoprotein cholesterol values in Soypro fed groups were similar compared to those of saline fed groups. Although further research is needed, these findings suggest that Soypro decreased the levels of low density lipoprotein cholesterol in high-fat diet-induced obesity and might partially inhibit the adipocyte differentiation through the suppression of a transcription factors peroxisome proliferator-activated receptor-gamma2 and CCAAT/enhancer binding protein-alpha.

Supplementation ofLactobacillus plantarumK68 and Fruit-Vegetable Ferment along with High Fat-Fructose Diet Attenuates Metabolic Syndrome in Rats with Insulin Resistance

“…Chronic high fat and fructose consumption, in terms of increased energy intake, has been shown a greater increase in bodyweights [23, 24]. Enhanced fat deposition with high fat-fructose diet may result in weight gain over a period of time.…”

“…In this context, controlled bodyweight by L. plantarum K68, FVF, and MIX supplements implies the reduced risk of CVD. Soypro, a fermented soymilk with lactic acid bacteria, isolated from Kimchi, was showed to inhibit the expression of transcription factors of adipocyte differentiation [23]. Yadav and colleagues [12] showed that dahi (curd), which contained lactobacillus bacteria prevent the bodyweight gain against high fructose diet.…”

Lactobacillus plantarumLG42 Isolated from Gajami Sik-Hae Inhibits Adipogenesis in 3T3-L1 Adipocyte

“…It is also reasonable to articulate that GLAB acts directly on PPAR γ and C/EBP α . Kim et al (2008) showed that treating 3T3-L1 cells with milk fermented with lactic acid bacteria isolated from kimchi decreased the levels of PPAR γ and C/EBP- α expression [28]. Thus, it could be possible that lactic acid bacteria isolated from gajami sik-hae affects the downstream genes of PPAR γ and C/EBP α , thus inhibiting adipogenesis in 3T3-L1 cells.…”

Health Benefits of Kimchi (Korean Fermented Vegetables) as a Probiotic Food

“…The fermented soymilk significantly decreased plasma LDL cholesterol in HFD-induced obesity in SD rats. 98 Sousa et al 99 reported that administration of Lactobacillus supernatants (LS) into the brains of rats resulted in weight loss without a decrease in food consumption, but with simultaneous increases of leptin expression in neurons and peripheral adipose tissue. LS also modulated the expression or release of other peptides involved in metabolism and body weight control.…”