Lipid Raft Alterations in Aged-Associated Neuropathologies

Marín, Raquel; Fabelo, Noemí; Fernández-Echevarría, Cecilia; Canerina-Amaro, Ana; Rodríguez‐Barreto, Deiene; Quinto-Alemany, David; Mesa-Herrera, Fátima; Dı́az, Mario

doi:10.2174/1567205013666160314150017

Cited by 33 publications

(40 citation statements)

References 140 publications

(208 reference statements)

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“…The reelin down-regulation observed in human occurs without change in Cask or TGF-β1 mRNA levels, but it is accompanied by increased levels of DNMT1 and ApoER2 mRNAs and decreased levels of HIC1 (Hypermethylated In Cancer 1) mRNA. Based in other reports [8,9], our observations suggest that decreased HIC1 expression activates that of ApoER2, which in turn would repress reelin expression. Therefore, the cancer associated reelin downregulation might result from DNMT1-mediated hypermethylation of its gene promoter and from HIC1-dependent increase in ApoER2 abundance [2 and unpublished observations].…”

Section: S2-4 Human Hmg-coa Lyases: Two Genes Three En-zymes and Onesupporting

confidence: 79%

“…, Fernández-Torre J.L. (3) , Ganfornina L. (8) , Giménez I. , Giráldez T. (4) , González C. (1) , Gual A. (2) , Guerra M. , Mediavilla M.D.…”

Section: S7-3unclassified

“…(3) , Montoro R.J. (6) , Palés J. (2) , Pié J. , Sánchez D. (8) , y Sánchez-Barceló E. (3) Universities: (1) Autónoma de Madrid, (2) Barcelona, (3) Cantabria, (4) La Laguna, (5) País Vasco, (6) Sevilla, (7) Valencia, (8) Valladolid y (9) Zaragoza The Inter-university Group for Innovative Physiology Teaching (GIDF) has been working over the last decade to educate teachers in methods for more effective teaching of Medical Physiology. This is particularly relevant after the launching of the European Higher Education Area and the Medical Degree in our Universities, whose goal is to train future doctors to provide quality clinical care in the realm of evidence-based Medicine.…”

Section: S7-3mentioning

confidence: 99%

“…By carrying TTE out, students recognized the useful of this method to evaluate the valves and chambers of the heart from the outside of the body. (1) , Alex Rafacho (2) , Paloma Alonso-Magdalena (3) , Manuel Castellano-Muñoz (4) , Eva María Bru-Tarí (5) , Beatriz Merino (6) , Antonia Ruiz (7) , Esperanza Irles (7) , Melisa Bello (6) , Pau Iborra (8) , Sabrina Villar (7) , Jean Vetorazzi (9) , Manuel Nacher , Ramón Luque (12) , Angel Nadal (7) , Ivan Quesada (7) (1) Departament of Physiology, Genetics and Microbiology, University of Alicante, Sant Vicent del Raspeig, Spain; (2) (CORT-14) is a neuropeptide mainly expressed in the Central Nervous System (CNS) that shows structural homology with somatostatin (SST). It was first discovered by controlling sleep physiology, but besides this central effect it displays many other actions in peripheral tissues including modulation of pancreatic islet function.…”

Section: Introductionmentioning

confidence: 99%

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Abstracts of the XXXVIII Congress of the Spanish Society of Physiological Sciences (SECF), 13-16 September 2016, Zaragoza, Spain

2016

J Physiol Biochem

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Dear colleagues,For the Organising Committee is an honour to host the XXXVIII Congress of the Spanish Society of Physiological Sciences (SECF) in Zaragoza. We are proud to welcome all of the participants to our city during this event that will take place between 13th and 16th September 2016.Throughout the different meetings, the SECF Congress has been considered an important instrument in attaining one of the main objectives of the Society, which is "to generate interest, stimulate research and disseminate knowledge of Physiology and its applications". We hope that this Congress, like all the previous ones, will offer us the opportunity to learn all about the current research activities of our colleagues in the field of physiology, encouraging contact between scientists and facilitating the exchange of information. Different studies will be presented in the form of plenary sessions, symposia and posters and will include superb scientific material that was carefully selected by the Scientific Committee. These studies, originating from laboratories from all over Spain, assure that the meeting will be a major scientific event. Topics cover a wide range of subjects, mainly related to Cell and Molecular Physiology, Neurophysiology, Endocrinology, Cardiovascular Physiology, Respiratory Physiology, Gastrointestinal Physiology, Renal Physiology, Nutrition, metabolism and obesity, Chronobiology, Bioengineering, Teaching of Physiology and Sports Physiology.As President of the Organising Committee, I greatly appreciate the effort of the contributing authors, and especially of the invited speakers, the chairpersons and colleagues from the Organising and Scientific Committees. Last but not least, I would like to express my gratitude to the commercial and institutional sponsors, without whose support it would have been impossible to organise this Congress.We hope this meeting will be fruitful, not only from the scientific point of view but also as a social event. Enjoy the hospitality of Zaragoza, its weather, the taste of its gastronomy and its rich history.Ignacio Giménez President of the Organising Committee J Physiol Biochem (2016) Micro-and nanotechnologies are strongly associated with the design and manufacture of the integrated circuits that are present in the chips of our computer or cell phone, being the basis of the actual technological revolution. Besides being so common in the devices of our daily life, in the near future they are foreseen to interconnect many everyday objects in what is called the Internet of things. These technologies are also an opportunity for many advances in medicine, not only for enhance the communications but to achieve smaller devices with many different features. Among the large amount of devices, it is noteworthy to highlight the pacemakers or cochlear implants. Micro and nanotechnology fabrication processes have achieved astonishing advances in manufacturing chips. I will show that these technologies are more accessible than what is believed and its implementation is possible to prod...

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Section: S2-4 Human Hmg-coa Lyases: Two Genes Three En-zymes and Onesupporting

confidence: 79%

“…, Fernández-Torre J.L. (3) , Ganfornina L. (8) , Giménez I. , Giráldez T. (4) , González C. (1) , Gual A. (2) , Guerra M. , Mediavilla M.D.…”

Section: S7-3unclassified

Section: S7-3mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Abstracts of the XXXVIII Congress of the Spanish Society of Physiological Sciences (SECF), 13-16 September 2016, Zaragoza, Spain

2016

J Physiol Biochem

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“…In our investigation, we have not measured the levels of cholesterol in the cells, but being under ideal and similar conditions, it is expected that there could be a significant increase in the levels of cholesterol in SH-SY5Y cells following 24 h in culture. Role of alteration in lipid raft dynamics due to cholesterol imbalance could be a mechanism involved in the neurotoxicity leading to PD and dementia with Lewy bodies [see for review; 24, 25]. It is evidenced from literature that in age-related disorders such as PD and Alzheimer’s disease, changes in lipid raft composition is observed from asymptomatic stage, which becomes important with physicochemical alteration toward progression of these diseases [25].…”

Section: Discussionmentioning

confidence: 99%

1-Methyl-4-Phenylpyridinium-Induced Death of Differentiated SH-SY5Y Neurons Is Potentiated by Cholesterol

Raju¹,

Jaisankar

Borah

et al. 2017

Ann Neurosci

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Background/Aims: Hypercholesterolemia is recently considered a risk factor for Parkinson’s disease (PD), the most consistent neurodegenerative movement disorder. The study aimed to investigate the effect of exogenous cholesterol on 1-methyl-4-phenylpyridinium (MPP⁺) parkinsonian neurotoxin-induced cell death, loss of mitochondrial membrane potential, and dopaminergic deficiency in a cellular model of PD. Methods: Cholesterol (50 μM) when added in the culture media alone or in combination with MPP⁺ was studied in SH-SY5Y neuroblastoma cells. There were 4 groups that were studied; SH-SY5Y cells treated with vehicle (control), cells that were treated with 1 mM MPP⁺ (MPP⁺), or cholesterol (chol) or both (M + chol). The loss of cell survival was measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Dopamine depletion, microtubule-associated protein 2 (MAP-2), and tyrosine hydroxylase (TH)-positive neuronal loss were determined by HPLC-electrochemical detection and TH immunocytochemistry respectively. Mitochondrial membrane potential in cells stained by tetramethylrhodamine methyl ester dye was analysed by flow cytometry. Results: Cholesterol treatment potentiated a reduction of neuronal viability with loss of TH-positive neurons in cultures. MPP⁺-induced depletion of dopamine level in the post-mitotic MAP-2 immunoreactive neurons and loss of mitochondrial membrane potential were also heightened by cholesterol. Conclusion: Apparently, changes in neuronal cholesterol content significantly influenced the neurotoxicity and the direct mitochondrial mechanisms involved in MPP⁺-induced cell death. Our observations demonstrate that high cholesterol incorporated into the differentiated human neuroblastoma cells worsened dopaminergic neuronal survivability through increased depolarization of mitochondrial membrane potential, which is a known mechanism of dopaminergic cell death by MPP⁺. The present findings support the hypothesis that hypercholesterolemia could be a risk factor for PD.

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Glial cell line‐derived neurotrophic factor alters lipid composition and protein distribution in MPP+‐injured differentiated SH‐SY5Y cells

Liu

Jiang

et al. 2020

Journal Cellular Physiology

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Parkinson's disease (PD) is a neurodegenerative disease characterized by progressive loss of dopaminergic neurons in the substantia nigra and striatum. Glial cell line‐derived neurotrophic factor (GDNF) can effectively promote the differentiation and survival of many types of neurons, especially dopaminergic neurons, suggesting it could be a treatment for PD. Lipid rafts are highly dynamic cell membrane domains that contain numerous signal protein receptors, providing an important platform for signal transduction. Compelling evidence indicates that alterations in lipid rafts are associated with PD, and some studies have reported that GDNF can regulate the expression of caveolin‐1, a lipid raft‐marker protein. However, the precise effects of GDNF on lipid rafts remain unknown. We developed a cellular PD model, purified detergent‐resistant membranes (membrane rafts), and performed proteomic and lipid metabolomics analyses to examine changes in lipid rafts after GDNF treatment. The results showed considerable protein and lipid alterations in response to GDNF, especially altered levels of dopamine‐β‐hydroxylase, heat shock 70 kDa protein, neural cell adhesion molecule, cytoskeletal proteins, and long‐chain polysaturated/unsaturated fatty acids. These findings reveal a new avenue to explore the relationships between GDNF, lipid rafts, and PD and support the hypothesis that GDNF may be a useful treatment for PD.

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Lipid Raft Alterations in Aged-Associated Neuropathologies

Cited by 33 publications

References 140 publications

Abstracts of the XXXVIII Congress of the Spanish Society of Physiological Sciences (SECF), 13-16 September 2016, Zaragoza, Spain

Abstracts of the XXXVIII Congress of the Spanish Society of Physiological Sciences (SECF), 13-16 September 2016, Zaragoza, Spain

1-Methyl-4-Phenylpyridinium-Induced Death of Differentiated SH-SY5Y Neurons Is Potentiated by Cholesterol

Glial cell line‐derived neurotrophic factor alters lipid composition and protein distribution in MPP+‐injured differentiated SH‐SY5Y cells

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