Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions

Babel, Laura; Kruse, Larissa; Bump, Steven; Langhans, Markus; Meckel, Tobias

doi:10.1186/s13104-017-3032-8

Cited by 5 publications

(3 citation statements)

References 32 publications

(26 reference statements)

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“…At the same time, the biochemical properties of RFs are similar to migrasome, which are rich in Actin and are lipid microdomains (Ma et al, 2015, Wu et al, 2017. Because the important concomitant ingrigrins of migrasome is also an important component of lipid microdomain (Babel et al, 2017). In summary, we believe that RFs have a very high similarity to migrasome.…”

Section: Plant Plasmodesmata and Animal Migrasomementioning

confidence: 64%

Plasmodesmata-like intercellular connections by plant remorin in animal cells

Wei

Tan

et al. 2019

Preprint

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Plants plasmodesmata (PD) are important structures for plant. The biogenesis of PD is an important and poorly understood process. Remorin is a highly conserved PD protein, but its contribution to the formation of PD is difficult to study because of the redundancy of remorin in plants and the importance of PD for plant cell survival. We found expression of remorin in animal cells mimics a functional PD-like structure by deforming the membranes into filamentous vesicles. After systematic verification and comparison studies, we found remorin converts the animal cell membrane into remorin filaments (RFs). Interestingly, these PD-like remorin filaments are similar to the migrasome, a kind of material transfer structure on the surface of animal cells, from multiple aspects. Our studies not only reveal the mechanism of PD biogenesis, but also explain the fundamental links of intercellular transport systems between plants and mammals. In addition, the unique character and effect of remorin indicate the potential biomedical applications.

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Section: Plant Plasmodesmata and Animal Migrasomementioning

confidence: 64%

Plasmodesmata-like intercellular connections by plant remorin in animal cells

Wei

Tan

et al. 2019

Preprint

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“…Further, a synthetic bile acid-phospholipid conjugate that inhibits integrin causes integrin internalization via lipid rafts [ 106 ]. Indeed, integrin β1 colocalizes with cholesterol in lipid rafts while ionizing radiation separates integrin β1 from cholesterol rafts [ 107 ]. Clearly, integrin adhesion and signaling correlates with the recruitment of these membrane proteins to lipid rafts.…”

Section: The Role Of Cholesterolmentioning

confidence: 99%

Roles of Membrane Domains in Integrin-Mediated Cell Adhesion

Lietha

Izard

2020

IJMS

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The composition and organization of the plasma membrane play important functional and regulatory roles in integrin signaling, which direct many physiological and pathological processes, such as development, wound healing, immunity, thrombosis, and cancer metastasis. Membranes are comprised of regions that are thick or thin owing to spontaneous partitioning of long-chain saturated lipids from short-chain polyunsaturated lipids into domains defined as ordered and liquid-disorder domains, respectively. Liquid-ordered domains are typically 100 nm in diameter and sometimes referred to as lipid rafts. We posit that integrin β senses membrane thickness and that mechanical force on the membrane regulates integrin activation through membrane thinning. This review examines what we know about the nature and mechanism of the interaction of integrins with the plasma membrane and its effects on regulating integrins and its binding partners.

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“…Some studies have demonstrated the role that lipid rafts play in radiotherapy resistance. A recent study has shown that the integrity of lipid rafts remains steady, even after radiation exposure [6]. Another recent study illustrates that radiation can enhance the integrity of lipid rafts in lung cancer cells, which might contribute to the development of radiation resistance [7].…”

Section: Introductionmentioning

confidence: 99%

10-Gingerol Targets Lipid Rafts Associated PI3K/Akt Signaling in Radio-Resistant Triple Negative Breast Cancer Cells

et al. 2020

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10-gingerol is a major phenolic lipid found in the rhizomes of ginger (Zingiber officinale). Being amphiphilic in nature, phenolic lipids have the ability to incorporate into cell membranes and modulate membrane properties. The purpose of the present study was to evaluate the effects of 10-gingerol on lipid raft/membrane raft modulation in radio-resistant triple negative breast cancer (MDA-MB-231/IR) cells. The effects of 10-gingerol on MDA-MB-231/IR cells’ proliferation, clonogenic growth, migration, and invasion were assayed using MTT, colony formation, cell migration, and invasion assays, respectively. Sucrose density gradient centrifugation was used to extract lipid rafts. Western blotting and immunofluorescence were employed to assess the effects of 10-gingerol on lipid raft/membrane raft modulation and lipid rafts-associated PI3K/Akt signaling. Cholesterol measurements were carried out using a commercially available kit. 10-gingerol suppressed the proliferation, migration, invasion, and induced apoptosis through targeting the PI3K/Akt signaling pathway in MDA-MB-231/IR cells. Moreover, 10-gingerol was found to modulate the lipid rafts of MDA-MB-231/IR cells and attenuate the key PI3K/Akt signaling components in lipid rafts. The cholesterol content of the lipid rafts and rafts-resident Akt signaling were also affected by exposure to 10-gingerol. The results of the present study highlight rafts-associated PI3K/Akt signaling as a new target of 10-gingerol in MDA-MB-231/IR cells, thus rationalizing a new rafts-mediated treatment approach for radio-resistant triple negative breast cancer cells.

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Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions

Cited by 5 publications

References 32 publications

Plasmodesmata-like intercellular connections by plant remorin in animal cells

Plasmodesmata-like intercellular connections by plant remorin in animal cells

Roles of Membrane Domains in Integrin-Mediated Cell Adhesion

10-Gingerol Targets Lipid Rafts Associated PI3K/Akt Signaling in Radio-Resistant Triple Negative Breast Cancer Cells

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