Lipid recovery from oleaginous yeasts: Perspectives and challenges for industrial applications

Khot, Mahesh; Raut, Gouri; Ghosh, Debashish; Alarcón, Manuel; Contreras, David; RaviKumar, Ameeta

doi:10.1016/j.fuel.2019.116292

Cited by 74 publications

(45 citation statements)

References 126 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, the influence of the cell envelopes surrounding the cytoplasmatic membrane on electroporation by PEF is unclear, but it has suggested that cell wall could influence the sensitivity to PEF (Aronsson et al, 2005;García et al, 2007). The highest PEF resistance could be related with the fact that oleaginous yeasts such as of X. dendrorhous have a higher fraction of chitin and/or mannan in the cell wall compared to non-oleaginous species that contribute to the rigidity and resistance to cell lysis (Khot et al, 2020). This rigidity and resistance to cell lysis should be higher in the ATCC 74219 strain to contain around 10 times more of carotenoids than those contained in the CECT 11028.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The aim of this study was to evaluate the potential of pulsed electric fields (PEF) to improve the extraction of the lipid-soluble astaxanthin from fresh biomass of a wild-type (CECT 11028) and mutant (ATCC 74219) Xanthophyllomyces dendrorhous strain using ethanol as solvent. Inactivation and propidium uptake studies revealed that inactivation is a good index for estimated the proportion of irreversible permeabilized cells when inactivation is higher than 70% in the two strains. Ethanol was ineffective for extracting carotenoids from the PEF-treated cells (20 kV/cm, 135 µs) of the two strains. However, after aqueous incubation of PEF-treated X. dendrorhous ATCC 74219 cells for 12 h, up to 2.4 ± 0.05 mg/g dried weight (d.w.) of carotenoids were extracted in ethanol. From total carotenoid extracted, around 84% corresponded to all-trans astaxanthin. The detection and quantification of esterase activity in the supernatant and the relationship between the percentage of esterase activity quantified and the amount of carotenoids extracted indicate that the extraction of astaxanthin was mediated by enzymatic esterase activity triggered by PEF during incubation. On the other hand, the formation of a large lipid globule into the cytoplasm of PEF-treated X. dendrorhous CECT 11028 cells during aqueous incubation prevented carotenoid extraction. The process developed in this investigation represents a more sustainable and greener method that those previously used for extracting astaxanthin from yeast.

show abstract

Section: Discussionmentioning

confidence: 99%

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…In order to extract the lipid from the microbe, the primary requisite is the disruption of the cell wall. Various techniques such as high-pressure homogenization, bead mill, ultrasound, pulsed electric field, osmotic shock, subcritical water hydrolysis, microwaves, enzymatic hydrolysis, autolysis, and chemical hydrolysis are generally used for cell disruption ( Khot et al, 2020 ). Besides, hydroxyl radicals, nanoscalpels, and photocatalytically active amino clay-conjugated TiO 2 are being used for cell disruption ( Dong et al, 2016 ; Chintagunta et al, 2020 ).…”

Section: Lipid Extraction and Transesterificationmentioning

confidence: 99%

Biodiesel Production From Lignocellulosic Biomass Using Oleaginous Microbes: Prospects for Integrated Biofuel Production

et al. 2021

View full text Add to dashboard Cite

Biodiesel is an eco-friendly, renewable, and potential liquid biofuel mitigating greenhouse gas emissions. Biodiesel has been produced initially from vegetable oils, non-edible oils, and waste oils. However, these feedstocks have several disadvantages such as requirement of land and labor and remain expensive. Similarly, in reference to waste oils, the feedstock content is succinct in supply and unable to meet the demand. Recent studies demonstrated utilization of lignocellulosic substrates for biodiesel production using oleaginous microorganisms. These microbes accumulate higher lipid content under stress conditions, whose lipid composition is similar to vegetable oils. In this paper, feedstocks used for biodiesel production such as vegetable oils, non-edible oils, oleaginous microalgae, fungi, yeast, and bacteria have been illustrated. Thereafter, steps enumerated in biodiesel production from lignocellulosic substrates through pretreatment, saccharification and oleaginous microbe-mediated fermentation, lipid extraction, transesterification, and purification of biodiesel are discussed. Besides, the importance of metabolic engineering in ensuring biofuels and biorefinery and a brief note on integration of liquid biofuels have been included that have significant importance in terms of circular economy aspects.

show abstract

“…In such high-latitude regions as Alaska and Siberia, the climate is too cold to grow any plants, which limits the potential of these regions as sources of second-generation biodiesel; third-generation biodiesel can solve this problem because its feedstocks are oils from oleaginous microorganisms, defined as those that can accumulate more than 20% lipids in their biomass. [13,16] Although interest in microbial lipids goes back over 140 years, [17] industrial-scale production of microbial oil has a history of only 35 years. In 1985, J.…”

Section: Feedstocksmentioning

confidence: 99%

“…Yeast lipids often include diacyl-and triacylglycerols (DAGs and TAGs), free fatty acids, sterols, polyphenols, and carotenoids, of which TAGs account for 80-90% of the total. [16] Lipid content varies depending on the medium. The common genera are Rhodotorula, Trichosporon, Yarrowia, and Lipomyces.…”

Section: Yeastmentioning

confidence: 99%

Biodiesel from Microorganisms: A Review

Chen

Nie

et al. 2021

Energy Tech

View full text Add to dashboard Cite

As societies develop and industrialize, sources of energy such as natural gas, coal, and oil become increasingly important in daily life. Among all forms of energy, fossil fuels are the most important, being primary sources of energy, [1] and, given their high heating value and therefore high efficiency, indispensable to heating, transport, and manufacturing operations. Fossil fuels most frequently seen in daily life are oil, coal, and natural gas. In 2019, total fossil energy consumption in the world was 492.34 exajoules (1 exajoule ¼ 10 18 J), accounting for 84.3% of the total energy. [2] Global demand for fossil fuels is predicted to increase to 19.08 billion liters a day by 2040. [3] However, fossil fuels are nonrenewable, and their use is also associated with many other problems such as air pollution, global warming, and unsustainable development. By 2030, the concentration of the greenhouse gas CO 2 is predicted to increase by 80% of the level in 2007, [4] which will contribute a great deal to global warming. These considerations make it essential for researchers to find alternatives to fossil fuels.The USA Energy Independence and Security Act declared that 136.27 billion liters of renewable fuel will be produced by 2022 in USA, including 3.78 billion liters of biodiesel, 60.56 billion liters of cellulosic biofuels, and 56.78 billion liters of maizebased ethanol. [5] Among all the suitable replacements, biofuels have the highest potential, because they can use waste material as feedstock, and they are renewable. In 2019, world production of biofuels was 111.83 million liters of oil equivalent per day, and the growth rate was 5.1% more than that in 2018. [6] Biofuels include bioethanol, biodiesel, biogas, biohydrogen, etc. [7] Forest waste, waste cooking oil, and similar materials can be used for producing biodiesel. In recent decades, microorganisms have been tested for their suitability as feedstock to produce biodiesel, and have proved more advantageous than other materials. The properties of biodiesel are similar to, and in some cases better than, those of diesel. For example, biodiesel has a higher cetane number, a higher flash point, and almost no sulfur, unlike diesel. When used in engines, biodiesel emits smaller quantities of particulate matter and carbon monoxide. [8] In addition, biodiesel can be mixed with fossil diesel in any proportion. Biodiesel is the predominant biofuel in Europe and the Asia Pacific and accounted for 81% and 74% of the total biofuels, respectively, used in these regions in 2019. [2] World production of biofuels from 1999 to 2019 is shown in Figure 1, and 2 shows the production of ethanol and biodiesel in 2009 and 2019.However, many external and internal facts hinder the development of biodiesel. For example, biodiesel is less competitive than diesel in terms of price and also inferior to diesel in some ways; the oxygen content of biodiesel lowers its heat value; and some forms of biodiesel also emit larger quantities of particulate matter (PM) and NO x . These drawbacks mus...

show abstract

Lipid recovery from oleaginous yeasts: Perspectives and challenges for industrial applications

Cited by 74 publications

References 126 publications

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Biodiesel Production From Lignocellulosic Biomass Using Oleaginous Microbes: Prospects for Integrated Biofuel Production

Biodiesel from Microorganisms: A Review

Contact Info

Product

Resources

About