Lipid Trafficking at Membrane Contact Sites During Plant Development and Stress Response

Michaud, Morgane; Jouhet, Juliette

doi:10.3389/fpls.2019.00002

Cited by 76 publications

(70 citation statements)

References 105 publications

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“…The stress generating ethylene, when adjacent to the chloroplast, degrades lipids in the cell membrane and activates chlorophyllase (chlase) gene [25]. Activation of chlorophyllase results in the degradation of chlorophyll, and ultimately plant suffers from chlorosis [25]. In the current study, without PLB (and nutritional stress), the chlorophyll contents were also low, which might be due to the production of ethylene, which was not investigated in this study.…”

Section: Discussionmentioning

confidence: 57%

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Pongamia pinnata L. Leaves Biochar Increased Growth and Pigments Syntheses in Pisum sativum L. Exposed to Nutritional Stress

et al. 2019

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Pea (Pisum sativum L.) leaf chlorophyll and pigments syntheses are retarded under nutritional stress. Biochar has the potential to regulate soil nutrient supplies and optimize plant nutrient uptakes. We examine the role of Pongamia pinnata L. waste leaf biochar (PLB) in improving vegetative growth and leaf chlorophyll and accessory pigments of pea exposed to nutritional stress. Three PLB application rates (0, 1, and 2%) crossed with half (HF), and full NPK fertilizer (FF) recommended doses were applied to sandy soil field-pots (arranged in a completely randomized design). There were significant or maximum increases in plant vegetative or physiological traits, including the fresh or dry, above- and below-ground biomass weights, and photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, and anthocyanin) in response to a 2%PLB + FF application (p = 0.002). Trait values also responded to 2%PLB + HF, which signified the nutrient regulatory character of PLB (p = 0.038). The PLB-driven reduction in nutritional stress resulted in diminished lycopene (antioxidant) content (p = 0.041). Therefore, we suggest that the soil application of 2%PLB + FF has the greatest impact on pea vegetative growth and leaf chlorophyll, carotenoids, anthocyanin, and lycopene contents in Pisum sativum L. Further research is recommended to investigate the relationship of PLB with soil nutrient availabilities and plant nutrient concentrations.

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Section: Discussionmentioning

confidence: 57%

“…Under nutritional stress, plants accumulate stress ethylene [24]. The stress generating ethylene, when adjacent to the chloroplast, degrades lipids in the cell membrane and activates chlorophyllase (chlase) gene [25]. Activation of chlorophyllase results in the degradation of chlorophyll, and ultimately plant suffers from chlorosis [25].…”

Section: Discussionmentioning

confidence: 99%

Pongamia pinnata L. Leaves Biochar Increased Growth and Pigments Syntheses in Pisum sativum L. Exposed to Nutritional Stress

et al. 2019

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“…VAPs have been extensively characterized in yeast and mammals, and more recently in plants, where they have wellestablished roles in the formation of MCSs between ER and other organelles, as well as facilitating the transfer of lipids between membranes (Lev et al, 2008;Prinz, 2014;Wang et al, 2017;Michaud and Jouhet, 2019). The potential for the presence of VAPs at the ER-LD junction may not be entirely unexpected, and there are interesting similarities and differences when comparing other MCSs of VAPs with the ER-LD junction.…”

Section: Discussionmentioning

confidence: 99%

SEIPIN Isoforms Interact with the Membrane-Tethering Protein VAP27-1 for Lipid Droplet Formation

et al. 2020

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SEIPIN proteins are localized to endoplasmic reticulum (ER)-lipid droplet (LD) junctions where they mediate the directional formation of LDs into the cytoplasm in eukaryotic cells. Unlike in animal and yeast cells, which have single SEIPIN genes, plants have three distinct SEIPIN isoforms encoded by separate genes. The mechanism of SEIPIN action remains poorly understood, and here we demonstrate that part of the function of two SEIPIN isoforms in Arabidopsis (Arabidopsis thaliana), AtSEIPIN2 and AtSEIPIN3, may depend on their interaction with the vesicle-associated membrane protein (VAMP)-associated protein (VAP) family member AtVAP27-1. VAPs have well-established roles in the formation of membrane contact sites and lipid transfer between the ER and other organelles, and here, we used a combination of biochemical, cell biology, and genetics approaches to show that AtVAP27-1 interacts with the N termini of AtSEIPIN2 and AtSEIPIN3 and likely supports the normal formation of LDs. This insight indicates that the ER membrane tethering machinery in plant cells could play a role with select SEIPIN isoforms in LD biogenesis at the ER, and additional experimental evidence in Saccharomyces cerevisiae supports the possibility that this interaction may be important in other eukaryotic systems.

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“…In addition, mechanisms of translocation of membrane lipids to other membrane sites or intracellular binding proteins had been proposed. For example, lipid trafficking might occur intracellularly through vesicular trafficking or through membrane-membrane contact sites between organelles [31]. Alternatively, glycolipid transfer protein could bind GSL molecules through association to plasma membrane, thereby inducing the translocation of membrane GSLs to the binding site in protein [6].…”

Section: Mechanism Of Proangiogenic Activity Conducted Bymentioning

confidence: 99%

Dissecting the conformation of glycans and their interactions with proteins

Wang

Lee

et al. 2020

J Biomed Sci

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The use of in silico strategies to develop the structural basis for a rational optimization of glycan-protein interactions remains a great challenge. This problem derives, in part, from the lack of technologies to quantitatively and qualitatively assess the complex assembling between a glycan and the targeted protein molecule. Since there is an unmet need for developing new sugar-targeted therapeutics, many investigators are searching for technology platforms to elucidate various types of molecular interactions within glycan-protein complexes and aid in the development of glycan-targeted therapies. Here we discuss three important technology platforms commonly used in the assessment of the complex assembly of glycosylated biomolecules, such as glycoproteins or glycosphingolipids: Biacore analysis, molecular docking, and molecular dynamics simulations. We will also discuss the structural investigation of glycosylated biomolecules, including conformational changes of glycans and their impact on molecular interactions within the glycan-protein complex. For glycoproteins, secreted protein acidic and rich in cysteine (SPARC), which is associated with various lung disorders, such as chronic obstructive pulmonary disease (COPD) and lung cancer, will be taken as an example showing that the core fucosylation of N-glycan in SPARC regulates protein-binding affinity with extracellular matrix collagen. For glycosphingolipids (GSLs), Globo H ceramide, an important tumor-associated GSL which is being actively investigated as a target for new cancer immunotherapies, will be used to demonstrate how glycan structure plays a significant role in enhancing angiogenesis in tumor microenvironments.

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Lipid Trafficking at Membrane Contact Sites During Plant Development and Stress Response

Cited by 76 publications

References 105 publications

Pongamia pinnata L. Leaves Biochar Increased Growth and Pigments Syntheses in Pisum sativum L. Exposed to Nutritional Stress

Pongamia pinnata L. Leaves Biochar Increased Growth and Pigments Syntheses in Pisum sativum L. Exposed to Nutritional Stress

SEIPIN Isoforms Interact with the Membrane-Tethering Protein VAP27-1 for Lipid Droplet Formation

Dissecting the conformation of glycans and their interactions with proteins

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