Lipid vesicles in capillary electrophoretic techniques: Characterization of structural properties and associated membrane‐molecule interactions

Owen, Rebecca L.; Strasters, Joost K.; Breyer, Emelita D.

doi:10.1002/elps.200410288

Cited by 61 publications

(33 citation statements)

References 136 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, CE was used to separate human organelles including mitochondria, acidic organelles, nuclei, and lipid vesicles (Gunasekera, Musier-Forsyth, & Arriaga, 2002;Duffy et al, 2002;Fuller & Arriaga, 2003;Owen, Strasters, & Breyer, 2005). The electrophoretic mobility of organelles is determined by the electrical charge on the surface of organelles, their morphology, and size (Owen, Strasters, & Breyer, 2005).…”

Section: Proteomics In Bacterial Outer Membrane Vesiclesmentioning

confidence: 99%

Proteomics in gram‐negative bacterial outer membrane vesicles

Lee

Choi

Kim

et al. 2008

Mass Spectrometry Reviews

287

277

View full text Add to dashboard Cite

Gram-negative bacteria constitutively secrete outer membrane vesicles (OMVs) into the extracellular milieu. Recent research in this area has revealed that OMVs may act as intercellular communicasomes in polyspecies communities by enhancing bacterial survival and pathogenesis in hosts. However, the mechanisms of vesicle formation and the pathophysiological roles of OMVs have not been clearly defined. While it is obvious that mass spectrometry-based proteomics offers great opportunities for improving our knowledge of bacterial OMVs, limited proteomic data are available for OMVs. The present review aims to give an overview of the previous biochemical, biological, and proteomic studies in the emerging field of bacterial OMVs, and to give future directions for high-throughput and comparative proteomic studies of OMVs that originate from diverse Gram-negative bacteria under various environmental conditions. This article will hopefully stimulate further efforts to construct a comprehensive proteome database of bacterial OMVs that will help us not only to elucidate the biogenesis and functions of OMVs but also to develop diagnostic tools, vaccines, and antibiotics effective against pathogenic bacteria.

show abstract

Section: Proteomics In Bacterial Outer Membrane Vesiclesmentioning

confidence: 99%

Proteomics in gram‐negative bacterial outer membrane vesicles

Lee

Choi

Kim

et al. 2008

Mass Spectrometry Reviews

287

277

View full text Add to dashboard Cite

show abstract

“…[56] EC experiments have been used to characterize the size, surface properties, encapsulation volumes, and the electrophoretic mobility of colloidal lipid vesicles and of lipoprotein particles. Interactions between biologically-related compounds and lipid vesicles that serve as pseudostationary phases, or as coated stationary phases, in electrokinetic chromatography, can be used to investigate the biophysical nature of drug-membrane model interactions.…”

Section: Analytical Techniques Available To Study Drug-membrane Modelmentioning

confidence: 99%

Biomembrane models and drug-biomembrane interaction studies: Involvement in drug design and development

et al. 2011

View full text Add to dashboard Cite

Contact with many different biological membranes goes along the destiny of a drug after its systemic administration. From the circulating macrophage cells to the vessel endothelium, to more complex absorption barriers, the interaction of a biomolecule with these membranes largely affects its rate and time of biodistribution in the body and at the target sites. Therefore, investigating the phenomena occurring on the cell membranes, as well as their different interaction with drugs in the physiological or pathological conditions, is important to exploit the molecular basis of many diseases and to identify new potential therapeutic strategies. Of course, the complexity of the structure and functions of biological and cell membranes, has pushed researchers toward the proposition and validation of simpler two- and three-dimensional membrane models, whose utility and drawbacks will be discussed. This review also describes the analytical methods used to look at the interactions among bioactive compounds with biological membrane models, with a particular accent on the calorimetric techniques. These studies can be considered as a powerful tool for medicinal chemistry and pharmaceutical technology, in the steps of designing new drugs and optimizing the activity and safety profile of compounds already used in the therapy.

show abstract

“…53 EC experiments have been used to characterize the size, surface properties, encapsulation volumes and electrophoretic mobility of colloidal lipid vesicles and lipoprotein particles. Interactions between biologically related compounds and lipid vesicles, which act as a pseudostationary phase or a coated stationary phase in electrokinetic chromatography, can be used to investigate the biophysical nature of drugmembrane model interactions.…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical methods for studying drug–biomembrane interactions

Pignatello

2013

Drug-Biomembrane Interaction Studies

View full text Add to dashboard Cite

Lipid vesicles in capillary electrophoretic techniques: Characterization of structural properties and associated membrane‐molecule interactions

Cited by 61 publications

References 136 publications

Proteomics in gram‐negative bacterial outer membrane vesicles

Proteomics in gram‐negative bacterial outer membrane vesicles

Biomembrane models and drug-biomembrane interaction studies: Involvement in drug design and development

Analytical methods for studying drug–biomembrane interactions

Contact Info

Product

Resources

About