LIPIDAT: A database of lipid phase transition temperatures and enthalpy changes. DMPC data subset analysis

Caffrey, Martin; Hogan, Jacqueline

doi:10.1016/0009-3084(92)90002-7

Cited by 131 publications

(103 citation statements)

References 3 publications

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“…It should be mentioned that we focused on the characterization of SUVs because the advantageous use of these liposomes over LUV in the spectroscopic experiments, where higher lipid concentrations are required, has been discussed elsewhere (21). The values of T C obtained by differential scanning calorimetry for SUV containing pure PS species are consistent with data for the gel to liquid crystalline phase transition for LUV and MLV of the same phospholipids found in the LIPIDAT data base (40). The T C values for the mixtures of EYPC and different molecular species of PS in the absence of BLA are lower than for the pure PS species (Table II).…”

Section: Interaction Of ␣-Lactalbumin With Liposomessupporting

confidence: 72%

The Interaction of Peripheral Proteins and Membranes Studied with α-Lactalbumin and Phospholipid Bilayers of Various Compositions

Agasøster

Halskau

Fuglebakk

et al. 2003

Journal of Biological Chemistry

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To characterize the interaction of peripheral proteins and membranes at the molecular level, we studied the reversible association of bovine ␣-lactalbumin (BLA) with lipid bilayers composed of different molecular forms of phosphatidylserine or equimolar mixtures of these phosphatidylserine forms and egg yolk phosphatidylcholine. At pH 4.5, almost all BLA (>90%) associates to negatively charged small unilamellar vesicles. The conformational changes that binding to these bilayers induced on the protein were characterized by circular dichroism and fluorescence spectroscopy. Because binding of BLA to negatively charged vesicles is reverted by adjusting the pH back to >6.0, we also investigated the conformation of the membrane-bound protein by NMRmonitored H-D exchange of the backbone amide protons. The conformation adopted by BLA bound to these bilayers resembles a molten globule-like state but the negative ellipticity at 222 nm and the apparent ␣-helix content of the bound protein senses the changes in the physical properties of the membrane. Binding to bilayers in the gel state appears to correlate with an increased amount of ␣-helical structure and with a lower extent of integration into the membrane, corresponding to the adsorbed protein, while the opposite is found for BLA bound to vesicles in the liquid-crystalline phase, corresponding to the embedded conformation. A common feature for the membrane-bound conformations of BLA is that the amphipathic helix C (residues 86 to 99) is an important determinant for the adsorption and further integration of the protein into the membrane.

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Section: Interaction Of ␣-Lactalbumin With Liposomessupporting

confidence: 72%

The Interaction of Peripheral Proteins and Membranes Studied with α-Lactalbumin and Phospholipid Bilayers of Various Compositions

Agasøster

Halskau

Fuglebakk

et al. 2003

Journal of Biological Chemistry

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“…These shorthand names lend themselves to fast, efficient text-based searches and are used widely in lipid research as compact alternatives to systematic names. The glycerophospholipids in the LIPIDAT database, for example, may be conveniently searched with a shorthand notation that has been extended to handle side chains with acyl, ether, branched-chain, and other functional groups (7). We propose the use of a shorthand notation for selected lipid categories ( Table 3 ) that incorporates a …”

Section: Databasing Lipids Annotation and Functionmentioning

confidence: 99%

A comprehensive classification system for lipids

Fahy

Subramaniam

Brown

et al. 2005

Journal of Lipid Research

1,467

685

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Lipids are produced, transported, and recognized by the concerted actions of numerous enzymes, binding proteins, and receptors. A comprehensive analysis of lipid molecules, "lipidomics," in the context of genomics and proteomics is crucial to understanding cellular physiology and pathology; consequently, lipid biology has become a major research target of the postgenomic revolution and systems biology. To facilitate international communication about lipids, a comprehensive classification of lipids with a common platform that is compatible with informatics requirements has been developed to deal with the massive amounts of data that will be generated by our lipid community. As an initial step in this development, we divide lipids into eight categories (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, prenol lipids, saccharolipids, and polyketides) containing distinct classes and subclasses of molecules, The goal of collecting data on lipids using a "systems biology" approach to lipidomics requires the development of a comprehensive classification, nomenclature, and chemical representation system to accommodate the myriad lipids that exist in nature. Lipids have been loosely defined as biological substances that are generally hydrophobic in nature and in many cases soluble in organic solvents (1). These chemical properties cover a broad range of mole-

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“…The maxima of the recorded transition peaks correspond well to T m values reported in literature. 32,37,38 The inu-ence of polymer addition to the lipid vesicles is different depending on the type of polymer, the type of lipid and the molar mixing ratio. Fig.…”

Section: -32mentioning

confidence: 99%

Binding of amphiphilic and triphilic block copolymers to lipid model membranes: the role of perfluorinated moieties

et al. 2014

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A novel class of symmetric amphi-and triphilic (hydrophilic, lipophilic, fluorophilic) block copolymers has been investigated with respect to their interactions with lipid membranes. The amphiphilic triblock copolymer has the structure PGMA 20 -PPO 34 -PGMA 20 (GP) and it becomes triphilic after attaching perfluoroalkyl moieties (F9) to either end which leads to F 9 -PGMA 20 -PPO 34 -PGMA 20 -F 9 (F-GP). The hydrophobic poly(propylene oxide) (PPO) block is sufficiently long to span a lipid bilayer. The poly(glycerol monomethacrylate) (PGMA) blocks have a high propensity for hydrogen bonding. The hydrophobic and lipophobic perfluoroalkyl moieties have the tendency to phase segregate in aqueous as well as in hydrocarbon environments. We performed differential scanning calorimetry (DSC) measurements on polymer bound lipid vesicles under systematic variation of the bilayer thickness, the nature of the lipid headgroup, and the polymer concentration. The vesicles were composed of phosphatidylcholines (DMPC, DPPC, DAPC, DSPC) or phosphatidylethanolamines (DMPE, DPPE, POPE). We showed that GP as well as F-GP binding have membrane stabilizing and destabilizing components.PPO and F9 blocks insert into the hydrophobic part of the membrane concomitantly with PGMA block adsorption to the lipid headgroup layer. The F9 chains act as additional membrane anchors. The insertion of the PPO blocks of both GP and F-GP could be proven by 2D-NOESY NMR spectroscopy. By fluorescence microscopy we show that F-GP binding increases the porosity of POPC giant unilamellar vesicles (GUVs), allowing the influx of water soluble dyes as well as the translocation of the complete triphilic polymer and its accumulation at the GUV surface. These results open a new route for the rational design of membrane systems with specific properties.

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LIPIDAT: A database of lipid phase transition temperatures and enthalpy changes. DMPC data subset analysis

Cited by 131 publications

References 3 publications

The Interaction of Peripheral Proteins and Membranes Studied with α-Lactalbumin and Phospholipid Bilayers of Various Compositions

The Interaction of Peripheral Proteins and Membranes Studied with α-Lactalbumin and Phospholipid Bilayers of Various Compositions

A comprehensive classification system for lipids

Binding of amphiphilic and triphilic block copolymers to lipid model membranes: the role of perfluorinated moieties

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