Lipidic intramembranous particles

Verkleij, Arie J.; Momvers, C; Leunissen‐Bijvelt, J.; Ververgaert, P.H.J.Th.

doi:10.1038/279162a0

Cited by 204 publications

(104 citation statements)

References 27 publications

(33 reference statements)

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“…3C). In analogy with previous observations [8,10] these results strongly suggest the existence of lipidic particles of an inverted micellar nature in the total rod outer segment lipid 'liposomes' at 37°C. Lipids extracted from entire retinas, which have a similar phospholipid distribution but are less unsaturated [ 15], show a similar behaviour, albeit that the isotropic component is less explicit.…”

Section: *Phosphatidylethanolamine Amounts To About 45% Of the Phosphsupporting

confidence: 90%

“…Saturated species can only form a bilayer, while egg phosphatidylethanolamine adopts the hexagonal HH phase at about 25°C and dioleoylphosphatidylethanolamine at about 5 ° C. Other membrane lipids like cardiolipin [4,6,7] and monoglucosyldiacylglycerol [8,9] also adopt the hexagonal H H phase at physiological temperature. Most interestingly, in mixtures of these lipids with phosphatid choline, lipidic particles of an inverted micellar nature associated with the bilayer have been observed both by freeze-fracture electron microscopy and by 31P-NMR [9,10].…”

Section: Discussionmentioning

confidence: 99%

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A possible role of rhodopsin in maintaining bilayer structure in the photoreceptor membrane

Grip

Drenthe

Echteld

et al. 1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Summary31p-NMR measurements demonstrate that at 37 ° C, independent of the photolytic state of the photopigment rhodopsin, the lipids in the photoreceptormembrane are almost exclusively organised in a bilayer. In strong contrast, the 31P-NMR spectra of the extracted lipids are characteristic for the hexagonal HII phase and an isotropic phase. The isotropic phase is characterised by freeze-fracture electron microscopy as particles and pits on smooth surfaces, possibly indicating inverted micelles. These results suggest a structural role for rhodopsin in maintaining the photoreceptor membrane lipids in a bilayer configuration.It has recently been demonstrated that the lipid bilayer, while still the basic feature of biological membranes, is not the only conformational state in which the lipids in membranes can occur. 31P-NMR studies have presented strong evidence that in the endoplasmic reticulum of rat, bovine and rabbit liver the phospholipids can undergo isotropic motion, which suggests that inverted micellar structures may be present in conjunction with the bilayer [ 1 ]. Model studies of water-phospholipid mixtures show that the preferred phase depends on the type of lipid [ 2--6 ]. Under most conditions the thermodynamically most stable phase of phosphatidylcholine is the bilayer. Unsaturated phosphatidylethanolamine also prefers the bilayer conformation at lower temperatures, but at increasing temperature it undergoes a phase Abbreviation: Mops, 3-(N-morpholino)propanesulfonic acid.

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Section: *Phosphatidylethanolamine Amounts To About 45% Of the Phosphsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

A possible role of rhodopsin in maintaining bilayer structure in the photoreceptor membrane

Grip

Drenthe

Echteld

et al. 1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…However, in previous work [16], correlations have been observed between the occurrence of narrow spectral 3'P-NMR components and the detection of 'lipidic particles' [18] in freeze-fracture micrographs. As shown in Fig.…”

Section: Resultsmentioning

confidence: 89%

Structural properties of phospholipids in the rat liver inner mitochondrial membrane. A 31P-NMR study

Cullis

Kruijff

Hope

et al. 1980

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…As noted in earlier reports, mixtures of 'bilayer' lipids with hexagonal 'HII' phase lipids, including the total lipid extracts of various biological membranes, can exhibit a new organization known as the 'lipidic particle' [2--4] which has been suggested to reflect the presence of intra-bilayer inverted micelles [ 3,4]. This structure, visualized as particles and pits in freeze-fracture micrographs, is also accompanied by the appearance of a narrow, symmetric 31p.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous work we have suggested that lipidic particles reflect the presence of intra-hilayer inverted micellar lipid structures [3,4]. This hypothesis was based largely on the observations that mixtures of 'bilayer' lipids with lipids preferring the 'inverted' (i.e., polar head groups oriented inwards towards an aqueous channel) H n phase give rise to such structures [4].…”

Section: Discussionmentioning

confidence: 99%

The lipidic particle as an intermediate structure in membrane fusion processes and bilayer to hexagonal HII transitions

Verkleij¹,

Echteld²,

Gerritsen³

et al. 1980

Biochimica et Biophysica Acta (BBA) - Biomembranes

148

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SummarySmall unilamellar vesicles comprised of a mixture of phosphatidylethanolamine/phosphatidylcholine/cholesterol (3:1:2) fuse to form large multilamellar vesicles on increasing the temperature from 0 to 50°C. This event is associated with the appearance of lipidic particles at the fusion sites, consistent with a role as intermediary structures during the fusion process. Further, for phosphatidylcholine/cardiolipin (1 : 1) liposomes in the presence of Mn 2+ a direct relationship between lipidic particles and the hexagonal (HII) phase is demonstrated which suggests that lipidic particles can also occur as intermediaries between bilayer and hexagonal (HII) structures.

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Lipidic intramembranous particles

Cited by 204 publications

References 27 publications

A possible role of rhodopsin in maintaining bilayer structure in the photoreceptor membrane

A possible role of rhodopsin in maintaining bilayer structure in the photoreceptor membrane

Structural properties of phospholipids in the rat liver inner mitochondrial membrane. A 31P-NMR study

The lipidic particle as an intermediate structure in membrane fusion processes and bilayer to hexagonal HII transitions

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