Lipidomic adaptations of the Metarhizium robertsii strain in response to the presence of butyltin compounds

Abstract

Metarhizium robertsii, a butyltin-resistant filamentous fungus, can rapid and complete biodegradation of di- (DBT) and tributyltin (TBT) under conditions of intensive aeration and ascorbic acid supplementation. In this paper, lipidomic investigations were performed to find the membrane adaptations necessary for effective butyltins degradation. HPLC-MS/MS analysis showed that the phospholipid profile was greatly modified during M. robertsii batch cultivation (pO ≥ 20%), contributing to increased membrane fluidity and facilitated mass transfer, which could enhance butyltins biodegradation. Intensified biosynthesis of phospholipids, sphingolipids and ergosterol by the mycelia exposed to butyltins was noted. DIOC(3) fluorescence intensity for TBT-treated mycelium increased 9-fold pointing to membrane hyperpolarization. Fluorescent studies showed improved membrane rigidity and integrity in response to butyltins presence. Vitamin C supplementation restored membrane composition and dynamic properties, followed by supposed acceleration of transport of monobutyltin and its biodegradation thus protecting the M. robertsii cells against oxidative and nitrosative stress.

Entomopathogenic fungi: unconventional applications

“…Changes were also noted in the thickness and composition of the cell wall (Ró _ zalska et al 2014). Another EDC-dibutyltin-and the by-product of its dealkylation, monobutyltin, caused nitrosative and oxidative stress and changes in the lipid profiles of M. robertsii (Siewiera et al 2017a;Stolarek et al 2019).…”

“…The cell membrane fluidity of M. robertsii raises the possibility of this EPF to degrade toxic substances. A membrane with a less compact structure, and thus with higher permeability, allows the transport of toxic compounds into the cell, where they are degraded (Stolarek et al 2019).…”

“…Every day substances are being introduced in the environment that have an adverse effect on the balance of the ecosystem and living organisms. Fungi that inhabit the soil are also sensitive to contaminants (e.g., herbicides, insecticides, fungicides and heavy metals) that cause decreased or delayed growth, abnormalities of cellular metabolic pathways, and damage of cellular structures (Ró _ zalska et al 2014;Gola et al 2018;Szewczyk et al 2018;Stolarek et al 2019;Subbanna et al 2019). Adverse changes in the cells caused by these compounds prevent these microorganisms from playing their proper ecological role.…”

Avaliação Parcial De Indicadores Do Programa De Agricultura De Baixa Emissão De Carbono

“…Fungos deste grupo foram capazes de degradar, através de seus metabólitos, pesticidas, estrogênio sintético e compostos de organotina (SZEWCZYK et al, 2018;STOLAREK et al, 2019). Ainda obtendo êxito como removedor de poluentes, em um trabalho realizado por Gola e colaboradores (2018), foi avaliada a capacidade de um isolado de B. bassiana degradar resíduos industriais e até compostos contendo metais pesados.…”

Agroecologia, Convivência Com O Semiárido E Questão Agrária Brasileira

“…São poucos os estudos, mas já são suficientes para pôr em evidência ainda mais os fungos entomopatogênicos como organismos extremamente versáteis, a variedade de aplicações é cada vez mais crescente. Recentemente foram reportados estudos que apresentam a interação destes com substâncias tóxicas ao meio ambiente, muitos compostos gerados pelo homem de forma natural ou sintética.Fungos deste grupo foram capazes de degradar, através de seus metabólitos, pesticidas, estrogênio sintético e compostos de organotina(SZEWCZYK et al, 2018;STOLAREK et al, 2019). Ainda obtendo êxito como removedor de poluentes, em um trabalho realizado por Gola e colaboradores (2018), foi avaliada a capacidade de um isolado de B. bassiana degradar resíduos industriais e até compostos contendo metais pesados.…”