Lipidomic analysis of human plasma reveals ether-linked lipids that are elevated in morbidly obese humans compared to lean

Donovan, Elise L.; Pettine, Stefan; Hickey, Matthew S.; Hamilton, Karyn L.; Miller, Benjamin F.

doi:10.1186/1758-5996-5-24

Cited by 63 publications

(63 citation statements)

References 46 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This aligns with previous work by Fernández-Real et al ., which reported that FAs expressed as relative % values distinguished lean and obese individuals more strongly than when FAs were expressed as absolute values [12]. The outcome of our discriminant analysis modelling was also similar to that previously shown by Donovan et al, who reported a similar scatter plot distribution when using FA profiling to distinguish lean from morbidly obese individuals [41]. Of interest, it was noted that while the LH group in our study was clearly distinct from both of the obese groups, the classification of MHO and MUO individuals showed some overlap.…”

Section: Discussionsupporting

confidence: 89%

A Distinct Fatty Acid Profile Underlies the Reduced Inflammatory State of Metabolically Healthy Obese Individuals

et al. 2014

View full text Add to dashboard Cite

BackgroundObesity is associated with numerous health complications; however, a subgroup of obese individuals (termed the metabolically healthy obese or MHO) appear to have lower risk for complications such as type 2 diabetes and cardiovascular disease. Emerging evidence suggests that MHO individuals have reduced inflammation compared to their metabolically unhealthy obese (MUO) counterparts. As it is recognized that fatty acids (FAs) have a strong relationship with inflammation, the current study aimed to uncover if the reduced inflammation observed in MHO individuals is mirrored by a more favourable FA profile.MethodsFasted serum samples were collected from lean healthy (LH), MHO, and MUO participants (n = 10/group) recruited from the Diabetes Risk Assessment study. A panel of pro- and anti-inflammatory markers were measured by immunoassay. Total serum FA profiling, as well as the FA composition of circulating phospholipids (PL) and triglycerides (TG), was measured by gas chromatography. ANOVA and Mann-Whitney-Wilcoxon tests were used to assess statistical significance between the groups (P<0.05).ResultsMHO and MUO individuals had similar BMI and body fat %; however, lipid parameters in MHO individuals more closely resembled that of LH individuals. MHO individuals had circulating levels of high sensitivity C-reactive protein (hsCRP) and interleukin-6 (IL-6) similar to LH individuals, while levels of platelet derived growth factor-ββ (PDGF-ββ) were intermediate to that of LH and MUO individuals. FA profiling analysis combined with discriminant analysis modelling highlighted a panel of nine FAs (consisting of three saturated, three monounsaturated, and three polyunsaturated FAs) in PL and TG fractions that distinguished the three groups. Specifically, saturated FA (myristic and stearic acids) levels in MHO individuals resembled that of LH individuals.ConclusionOur results suggest that the reduced inflammatory state of MHO individuals compared to MUO individuals may stem, in part, from a more favourable underlying FA profile.

show abstract

Section: Discussionsupporting

confidence: 89%

A Distinct Fatty Acid Profile Underlies the Reduced Inflammatory State of Metabolically Healthy Obese Individuals

et al. 2014

View full text Add to dashboard Cite

show abstract

“…This results from difficulties in determination of these compounds, associated either with their complex chemical characteristics or with the lack of appropriate instrumentation. Lipidomics is broadly defined as a structural and functional analysis of lipids and their metabolites .…”

Section: Introductionmentioning

confidence: 99%

Alterations of specific lipid groups in serum of obese humans: a review

Mika

Śledziński

2016

Obesity Reviews

View full text Add to dashboard Cite

Obesity is a major contributor to the dysfunction of liver, cardiac, pulmonary, endocrine and reproductive system, as well as a component of metabolic syndrome. Although development of obesity-related disorders is associated with lipid abnormalities, most previous studies dealing with the problem in question were limited to routinely determined parameters, such as serum concentrations of triacylglycerols, total cholesterol, low-density and high-density lipoprotein cholesterol. Many authors postulated to extend the scope of analysed lipid compounds and to study obesity-related alterations in other, previously non-examined groups of lipids. Comprehensive quantitative, structural and functional analysis of specific lipid groups may result in identification of new obesity-related alterations. The review summarizes available evidence of obesity-related alterations in various groups of lipids and their impact on health status of obese subjects. Further, the role of diet and endogenous lipid synthesis in the development of serum lipid alterations is discussed, along with potential application of various lipid compounds as risk markers for obesity-related comorbidities.

show abstract

“…In recent years, to simplify data processing, multivariate analysis techniques such as principal component analysis (PCA) and partial least squares of latent structures (PLS) have been used extensively in MS applications with the aim to find quantitative or qualitative differences between groups [17–18]. …”

Section: Introductionmentioning

confidence: 99%

Identification of oxidized phospholipids in bronchoalveolar lavage exposed to low ozone levels using multivariate analysis

Almstrand

Voelker

Murphy

2015

Analytical Biochemistry

View full text Add to dashboard Cite

Chemical reactions with unsaturated phospholipids in the respiratory tract lining fluid have been identified as one of the first important steps in the mechanisms mediating environmental ozone toxicity. As a consequence of these reactions, complex mixtures of oxidized lipids are generated in the presence of mixtures of non-oxidized naturally occurring phospholipid molecular species, which challenge methods of analysis. Untargeted mass spectrometry and statistical methods were employed to approach these complex spectra. Human bronchoalveolar lavage (BAL) was exposed to low levels of ozone and samples, with and without derivatization of aldehydes, were analyzed by liquid chromatography electrospray ionization tandem mass spectrometry. Data processing was carried out using principal component analysis (PCA). Resulting PCA score plots indicated an ozone dose-dependent increase, with apparent separation between BAL samples exposed to 60 ppb ozone and non-exposed BAL samples, and a clear separation between ozonized samples before and after derivatization. Corresponding loadings plots revealed that more than 30 phosphatidylcholine (PC) species decreased due to ozonation. A total of 13 PC and 6 phosphatidylglycerol oxidation products were identified with the majority being structurally characterized as chain-shortened aldehyde products. This method exemplifies an approach for comprehensive detection of low abundance, yet important, components in complex lipid samples.

show abstract

Lipidomic analysis of human plasma reveals ether-linked lipids that are elevated in morbidly obese humans compared to lean

Cited by 63 publications

References 46 publications

A Distinct Fatty Acid Profile Underlies the Reduced Inflammatory State of Metabolically Healthy Obese Individuals

A Distinct Fatty Acid Profile Underlies the Reduced Inflammatory State of Metabolically Healthy Obese Individuals

Alterations of specific lipid groups in serum of obese humans: a review

Identification of oxidized phospholipids in bronchoalveolar lavage exposed to low ozone levels using multivariate analysis

Contact Info

Product

Resources

About